scriptcap 2'-o-methyltransferase kit (Epicentre Biotechnologies)
90
Structured Review
Epicentre Biotechnologies
scriptcap 2'-o-methyltransferase kit
Scriptcap 2' O Methyltransferase Kit, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scriptcap+kit/scriptcap+2++o+methyltransferase+kit/pmc11530239-307-17-20
Average 90 stars, based on 1 article reviews
Scriptcap 2' O Methyltransferase Kit, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scriptcap+kit/scriptcap+2++o+methyltransferase+kit/pmc11530239-307-17-20
Average 90 stars, based on 1 article reviews
scriptcap 2'-o-methyltransferase kit - by Bioz Stars,
2026-09
90/100 stars
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In Vitro:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( Methylation:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( Incubation:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( Derivative Assay:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( Virus:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( Quantitative RT-PCR:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( Produced:Article Title: The C-terminal α–α superhelix of Pat is required for mRNA decapping in metazoa Article Snippet: The RNA probe was labelled with [α- 32 P]GTP using the Article Title: Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: Base-pairing promotes leader selection to prime in vitro influenza genome transcription. Article Snippet: These uncapped RNA leaders were subsequently provided cap-0 or cap-1 structures by using the Article Title: Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Article Snippet: The removal of the protective cap structure from an mRNA 5′ end in the process of decapping is a critical step in the regulation of gene expression, because it is generally an irreversible process that inhibits translation initiation and commits the mRNA to full degradation1,2.. Decapping is catalyzed by Dcp2, a bilobed hydrolase of the Nudix family3–5, which hydrolyzes the mRNA 5′-cap structure, thereby releasing m7GDP and a 5′-monophosphorylated mRNA.. Decapped mRNAs are in turn susceptible to full degradation by 5′-to-3′ exonucleases of the XRN1 family1,2. Article Title: Human DCP1 promotes mRNA decapping by enhancing the RNA binding affinity of DCP2 Article Snippet: To generate the 32 P-labeled cap RNA, we utilized the Article Title: In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation Article Snippet: Radiolabeled cap analog standards were produced by direct digestion of the substrates leading to GpppA and 7Me GpppA or digestion after methylation of the 2′O-position using VV 2′O-MTase ( Article Title: Ribose 2′- O -methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5 Article Snippet: In vitro 2′- O -methylation reactions were incubated for 1 h at 37 °C and included 300 ng poly(A)-containing RNA derived from virus-infected cells or the corresponding amount of poly(A)-containing RNA from uninfected cells (as determined by quantitative RT-PCR with primers specific for mouse GAPDH and human β-actin; data not shown) plus ScriptCap 2′- O -methyltransferase ( |