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Microbia Inc multiplex rna microarray platform midtal
Multiplex Rna Microarray Platform Midtal, supplied by Microbia Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+microarray+platform/midtal+microarray/pm28427011-90-8-17
Average 90 stars, based on 1 article reviews
multiplex rna microarray platform midtal - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Algae:

Article Title: Evolution of the MIDTAL microarray: the adaption and testing of oligonucleotide 18S and 28S rDNA probes and evaluation of subsequent microarray generations with Prymnesium spp. cultures and field samples.
Article Snippet: The toxic microalgal species Prymnesium parvum and Prymnesium polylepis are responsible for numerous fish kills causing economic stress on the aquaculture industry and, through the consumption of contaminated shellfish, can potentially impact on human health.. Monitoring of toxic phytoplankton is traditionally carried out by light microscopy.. However, molecular methods of identification and quantification are becoming more common place.

Article Title: Validation of the detection of Pseudo-nitzschia spp. using specific RNA probes tested in a microarray format: Calibration of signal based on variability of RNA content with environmental conditions
Article Snippet: Harmful algal blooms (HAB) occur worldwide and cause health problems and economic damage to fisheries and tourism.. Monitoring for toxic algae is therefore essential but is based primarily on light microscopy, which is time consuming and can be limited by insufficient morphological characters such that more time is needed to examine critical features with electron microscopy.. Monitoring with molecular tools is done in only a few places world-wide.

Article Title: Validation of the detection of Alexandrium species using specific RNA probes tested in a microarray format: Calibration of signal using variability of RNA content with environmental conditions
Article Snippet: .. The probe sequence for all probes designed or modified from FISH probes for the entire project for the MIDTAL microarray are patent pending for a universal microarray for the detection of toxic algae, and the entire hybridisation kit including the array and all necessary reagents are now commercially available from Microbia Environnement (France). ..

Microarray:

Article Title: Evolution of the MIDTAL microarray: the adaption and testing of oligonucleotide 18S and 28S rDNA probes and evaluation of subsequent microarray generations with Prymnesium spp. cultures and field samples.
Article Snippet: The toxic microalgal species Prymnesium parvum and Prymnesium polylepis are responsible for numerous fish kills causing economic stress on the aquaculture industry and, through the consumption of contaminated shellfish, can potentially impact on human health.. Monitoring of toxic phytoplankton is traditionally carried out by light microscopy.. However, molecular methods of identification and quantification are becoming more common place.

Article Title: Biosensors for the monitoring of harmful algal blooms.
Article Snippet: .. McCoy et al. [30 ,31] have described a multiplex RNA microarray platform (MIDTAL, currently available commercially from Microbia Environnement) that can detect and quantify numerous Alexandrium species. ..

Article Title: Validation of the detection of Pseudo-nitzschia spp. using specific RNA probes tested in a microarray format: Calibration of signal based on variability of RNA content with environmental conditions
Article Snippet: Harmful algal blooms (HAB) occur worldwide and cause health problems and economic damage to fisheries and tourism.. Monitoring for toxic algae is therefore essential but is based primarily on light microscopy, which is time consuming and can be limited by insufficient morphological characters such that more time is needed to examine critical features with electron microscopy.. Monitoring with molecular tools is done in only a few places world-wide.

Article Title: Advances in the Detection of Toxic Algae Using Electrochemical Biosensors
Article Snippet: .. The MIDTAL microarray, now marketed by Microbia Environnement, does not contain these species and is therefore not a universal platform. ..

Article Title: Validation of the detection of Alexandrium species using specific RNA probes tested in a microarray format: Calibration of signal using variability of RNA content with environmental conditions
Article Snippet: .. The probe sequence for all probes designed or modified from FISH probes for the entire project for the MIDTAL microarray are patent pending for a universal microarray for the detection of toxic algae, and the entire hybridisation kit including the array and all necessary reagents are now commercially available from Microbia Environnement (France). ..

Multiplex Assay:

Article Title: Biosensors for the monitoring of harmful algal blooms.
Article Snippet: .. McCoy et al. [30 ,31] have described a multiplex RNA microarray platform (MIDTAL, currently available commercially from Microbia Environnement) that can detect and quantify numerous Alexandrium species. ..

Sequencing:

Article Title: Validation of the detection of Pseudo-nitzschia spp. using specific RNA probes tested in a microarray format: Calibration of signal based on variability of RNA content with environmental conditions
Article Snippet: Harmful algal blooms (HAB) occur worldwide and cause health problems and economic damage to fisheries and tourism.. Monitoring for toxic algae is therefore essential but is based primarily on light microscopy, which is time consuming and can be limited by insufficient morphological characters such that more time is needed to examine critical features with electron microscopy.. Monitoring with molecular tools is done in only a few places world-wide.

Article Title: Validation of the detection of Alexandrium species using specific RNA probes tested in a microarray format: Calibration of signal using variability of RNA content with environmental conditions
Article Snippet: .. The probe sequence for all probes designed or modified from FISH probes for the entire project for the MIDTAL microarray are patent pending for a universal microarray for the detection of toxic algae, and the entire hybridisation kit including the array and all necessary reagents are now commercially available from Microbia Environnement (France). ..

Modification:

Article Title: Validation of the detection of Pseudo-nitzschia spp. using specific RNA probes tested in a microarray format: Calibration of signal based on variability of RNA content with environmental conditions
Article Snippet: Harmful algal blooms (HAB) occur worldwide and cause health problems and economic damage to fisheries and tourism.. Monitoring for toxic algae is therefore essential but is based primarily on light microscopy, which is time consuming and can be limited by insufficient morphological characters such that more time is needed to examine critical features with electron microscopy.. Monitoring with molecular tools is done in only a few places world-wide.

Article Title: Validation of the detection of Alexandrium species using specific RNA probes tested in a microarray format: Calibration of signal using variability of RNA content with environmental conditions
Article Snippet: .. The probe sequence for all probes designed or modified from FISH probes for the entire project for the MIDTAL microarray are patent pending for a universal microarray for the detection of toxic algae, and the entire hybridisation kit including the array and all necessary reagents are now commercially available from Microbia Environnement (France). ..

Fluorescence In Situ Hybridization:

Article Title: Validation of the detection of Pseudo-nitzschia spp. using specific RNA probes tested in a microarray format: Calibration of signal based on variability of RNA content with environmental conditions
Article Snippet: Harmful algal blooms (HAB) occur worldwide and cause health problems and economic damage to fisheries and tourism.. Monitoring for toxic algae is therefore essential but is based primarily on light microscopy, which is time consuming and can be limited by insufficient morphological characters such that more time is needed to examine critical features with electron microscopy.. Monitoring with molecular tools is done in only a few places world-wide.

Article Title: Validation of the detection of Alexandrium species using specific RNA probes tested in a microarray format: Calibration of signal using variability of RNA content with environmental conditions
Article Snippet: .. The probe sequence for all probes designed or modified from FISH probes for the entire project for the MIDTAL microarray are patent pending for a universal microarray for the detection of toxic algae, and the entire hybridisation kit including the array and all necessary reagents are now commercially available from Microbia Environnement (France). ..

Hybridization:

Article Title: Validation of the detection of Pseudo-nitzschia spp. using specific RNA probes tested in a microarray format: Calibration of signal based on variability of RNA content with environmental conditions
Article Snippet: Harmful algal blooms (HAB) occur worldwide and cause health problems and economic damage to fisheries and tourism.. Monitoring for toxic algae is therefore essential but is based primarily on light microscopy, which is time consuming and can be limited by insufficient morphological characters such that more time is needed to examine critical features with electron microscopy.. Monitoring with molecular tools is done in only a few places world-wide.

Article Title: Validation of the detection of Alexandrium species using specific RNA probes tested in a microarray format: Calibration of signal using variability of RNA content with environmental conditions
Article Snippet: .. The probe sequence for all probes designed or modified from FISH probes for the entire project for the MIDTAL microarray are patent pending for a universal microarray for the detection of toxic algae, and the entire hybridisation kit including the array and all necessary reagents are now commercially available from Microbia Environnement (France). ..



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Image Search Results


Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

Journal: PLoS ONE

Article Title: Scleral Micro-RNA Signatures in Adult and Fetal Eyes

doi: 10.1371/journal.pone.0078984

Figure Lengend Snippet: Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

Article Snippet: Genome-wide micro-RNA profiling was performed using the Agilent micro-RNA microarray platform.

Techniques: Microarray

( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

Journal: Cancers

Article Title: Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma

doi: 10.3390/cancers12041057

Figure Lengend Snippet: ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

Article Snippet: Gene expression profiling was performed in four Merkel cell carcinoma cell lines, using the Illumina RNA microarray platform.

Techniques: Microarray, Expressing, Western Blot, Over Expression, Colony Assay