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human long noncoding rna 4 × 180k microarray platform  (Agilent technologies)


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    Structured Review

    Agilent technologies human long noncoding rna 4 × 180k microarray platform
    Human Long Noncoding Rna 4 × 180k Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/pmc04757888-111-1-0
    Average 90 stars, based on 1 article reviews
    human long noncoding rna 4 × 180k microarray platform - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Abstracts from the 56 th European Society of Human Genetics (ESHG) Conference: e-Posters.
    Article Snippet: Genetic investigation was performed using 180K Agilent oligonucleotides array CGH analysis according to the manufacturer’s instructions.

    Article Title: KDM1A genotyping and expression in 146 sporadic somatotroph pituitary adenomas.
    Article Snippet: Importance: A paradoxical increase of growth hormone (GH) following oral glucose load has been described in ∼30% of patients with acromegaly and has been related to the ectopic expression of the glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) in somatotropinomas.. Recently, we identified germline pathogenic variants and somatic loss of heterozygosity of lysine demethylase 1A (KDM1A) in patients with GIP-dependent primary bilateral macronodular adrenal hyperplasia with Cushing's syndrome.. The ectopic expression of GIPR in both adrenal and pituitary lesions suggests a common molecular mechanism.

    Article Title: Clinical and molecular cytogenetic studies of five new patients with 20q11q12 deletion and review of the literature: Proposition of two critical regions.
    Article Snippet: It was conducted using oligo 60k Agilent® array for Patients #1, #2, and #3, and oligo 180k Agilent® array (Agilent technologies, Santa, Clara, CA, USA) for Patient #5.

    Microarray:

    Article Title: Phenotypic spectrum in Weiss-Kruszka syndrome caused by ZNF462 variants: Three new patients and literature review.
    Article Snippet: .. Array-CGH microarray utilized peripheral blood and 180k microarray slides from Agilent Technologies, following the manufacturer’s guidelines. .. Scanned images underwent processing through Agilent Genomic Workbench version 6.5 and Cartagenia software (Agilent Technologies).

    Article Title: Amplification of Mutant NRAS in Melanocytic Tumors With Features of Spitz Tumors.
    Article Snippet: .. For 5 cases, copy number variation was evaluated by array comparative genomic hybridization (CGH) on Agilent SurePrint G3 Human CGH Microarray 4 180K arrays with commercially available normal reference DNA. ..

    Article Title: DNA sequence and chromatin differentiate sequence-specific transcription factor binding in the human malaria parasite Plasmodium falciparum.
    Article Snippet: .. Overall, the total number of dsDNA probes was 174 550 spots, which were arrayed using an Agilent Technologies 4 × 180k microarray design. .. Overall, the total number of dsDNA probes was 174 550 spots, which were arrayed using an Agilent Technologies 4 × 180k microarray design.

    Article Title: DNA sequence and chromatin differentiate sequence-specific transcription factor binding in the human malaria parasite Plasmodium falciparum.
    Article Snippet: .. Additionally, each DNA probe was replicated in random areas of the microarray surface (four C AC AC A / GTGC AC replicates and three GT AC / TGCA TGCA replicates per orientation), which brought the total number of DNA probes to 174,550 spots for a 4 × 180k microarray (Agilent Technologies). ..

    Article Title: Novel PUF60 variant suggesting an interaction between Verheij and Cornelia de Lange syndrome: phenotype description and review of the literature
    Article Snippet: .. Array-CGH microarray involved the use of peripheral blood and 180k microarray slides from Agilent Technologies (Santa Clara, CA), following the manufacturer’s protocols. .. Scanned images were processed using Agilent Genomic Workbench version 6.5 and Cartagenia software (Agilent Technologies).

    Hybridization:

    Article Title: Amplification of Mutant NRAS in Melanocytic Tumors With Features of Spitz Tumors.
    Article Snippet: .. For 5 cases, copy number variation was evaluated by array comparative genomic hybridization (CGH) on Agilent SurePrint G3 Human CGH Microarray 4 180K arrays with commercially available normal reference DNA. ..



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    Agilent technologies human long noncoding rna 4 × 180k microarray platform
    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from <t>microarray</t> data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.
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    Image Search Results


    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

    Journal: PLoS ONE

    Article Title: Scleral Micro-RNA Signatures in Adult and Fetal Eyes

    doi: 10.1371/journal.pone.0078984

    Figure Lengend Snippet: Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

    Article Snippet: Genome-wide micro-RNA profiling was performed using the Agilent micro-RNA microarray platform.

    Techniques: Microarray

    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

    Journal: Cancers

    Article Title: Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma

    doi: 10.3390/cancers12041057

    Figure Lengend Snippet: ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

    Article Snippet: Gene expression profiling was performed in four Merkel cell carcinoma cell lines, using the Illumina RNA microarray platform.

    Techniques: Microarray, Expressing, Western Blot, Over Expression, Colony Assay