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smart mmlv reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa smart mmlv reverse transcriptase
    Smart Mmlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 5574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase/SMART+MMLV+Reverse+Transcriptase/pmc13652265-196-0-8
    Average 97 stars, based on 5574 article reviews
    smart mmlv reverse transcriptase - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: FoxL2 exhibits two-stage expression and maintains the ovarian niche in a lophotrochozoan
    Article Snippet: .. Briefly, first-strand cDNA was synthesized from 2 μg of total RNA using oligo(dT)18 primers and MMLV reverse transcriptase (TaKaRa, Japan) in a 20 μL reaction volume. ..

    Article Title: Evaluation of low-density polyethylene biodegradation by Bacillus safensis BS-10L after plasma treatment under environmental stress and underlying mechanisms
    Article Snippet: Total RNA was isolated from cultured bacterial cells using an RNA extraction kit (TAKARA, Tokyo, Japan). .. Complementary DNA (cDNA) was synthesized using SMART® MMLV Reverse Transcriptase (Clontech, Mountain View, California, USA) following the manufacturer’s instructions. .. One microliter of the synthesized cDNA was used as the template for quantitative reverse transcription polymerase chain reaction (qRT-PCR). qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad, Hercules, California, USA) and a Real-Time PCR detection system (Bio-Rad).

    Article Title: The effect of Enterococcus faecium L6 and Lactobacillus plantarum L10 combination on broiler growth and protection against enterohemorrhagic Escherichia coli O157:H7 challenge
    Article Snippet: .. Total RNA was extracted from jejunum with the High Purity RNA Tissue Kit (Takara, Japan) followed by purity assessment (OD260/280) on a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). cDNA was subsequently synthesized using the Reverse Transcriptase M-MLV Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was performed on a 7500 Real Time PCR system (Applied Biosystem, USA) with β-actin as the internal control. ..

    Article Title: Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis.
    Article Snippet: 2017).. Terpenoids widely participate in biotic and abiotic stress responses of plants (Pichersky and Raguso 2018).. For example, sesquiterpene β-caryophyllene suppresses the infection of Pseudomonas syringae into Arabidopsis thaliana (Kollner et al. 2008).

    Reverse Transcription:

    Article Title: FoxL2 exhibits two-stage expression and maintains the ovarian niche in a lophotrochozoan
    Article Snippet: .. Briefly, first-strand cDNA was synthesized from 2 μg of total RNA using oligo(dT)18 primers and MMLV reverse transcriptase (TaKaRa, Japan) in a 20 μL reaction volume. ..


    Article Title: Cakc2.1, an Ortholog of the Arabidopsis K + Channel KAT1, Positively Mediates Pepper Fruit Coloration.
    Article Snippet: Potassium (K+), the most abundant cellular cation, plays essential roles in various plant biological functions, such as stomatal movement and stress responses.. Although the molecular mechanisms of K+ uptake and transport have been established in the model plant Arabidopsis thaliana, in addition to other plant species such as rice and grape, little is known about these mechanisms in pepper.. In this study, CaKC2.1 was cloned from pepper (Capsicum annuum L.), which encodes a putative plant voltagegated K+ channel protein.

    Article Title: PCIF1-mediated m 6 Am modification in ACC neurons participates in inflammatory pain and anxiety.
    Article Snippet: Anesthetize mice with isoflurane, quickly remove the ACC, and store it at 80 ◦ C. According to the specifications (R401-01, Vazyme), use TRIzol reagent to extract total RNA from the ACC tissue. .. Then, we use the reverse transcriptase M-MLV (2641A, Takara) to reverse transcribe 400 ng of total RNA into cDNA. ..

    Article Title: Evaluation of low-density polyethylene biodegradation by Bacillus safensis BS-10L after plasma treatment under environmental stress and underlying mechanisms
    Article Snippet: Total RNA was isolated from cultured bacterial cells using an RNA extraction kit (TAKARA, Tokyo, Japan). .. Complementary DNA (cDNA) was synthesized using SMART® MMLV Reverse Transcriptase (Clontech, Mountain View, California, USA) following the manufacturer’s instructions. .. One microliter of the synthesized cDNA was used as the template for quantitative reverse transcription polymerase chain reaction (qRT-PCR). qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad, Hercules, California, USA) and a Real-Time PCR detection system (Bio-Rad).

    Article Title: The effect of Enterococcus faecium L6 and Lactobacillus plantarum L10 combination on broiler growth and protection against enterohemorrhagic Escherichia coli O157:H7 challenge
    Article Snippet: .. Total RNA was extracted from jejunum with the High Purity RNA Tissue Kit (Takara, Japan) followed by purity assessment (OD260/280) on a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). cDNA was subsequently synthesized using the Reverse Transcriptase M-MLV Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was performed on a 7500 Real Time PCR system (Applied Biosystem, USA) with β-actin as the internal control. ..

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Article Title: Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis.
    Article Snippet: 2017).. Terpenoids widely participate in biotic and abiotic stress responses of plants (Pichersky and Raguso 2018).. For example, sesquiterpene β-caryophyllene suppresses the infection of Pseudomonas syringae into Arabidopsis thaliana (Kollner et al. 2008).

    Spectrophotometry:

    Article Title: The effect of Enterococcus faecium L6 and Lactobacillus plantarum L10 combination on broiler growth and protection against enterohemorrhagic Escherichia coli O157:H7 challenge
    Article Snippet: .. Total RNA was extracted from jejunum with the High Purity RNA Tissue Kit (Takara, Japan) followed by purity assessment (OD260/280) on a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). cDNA was subsequently synthesized using the Reverse Transcriptase M-MLV Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was performed on a 7500 Real Time PCR system (Applied Biosystem, USA) with β-actin as the internal control. ..

    Quantitative RT-PCR:

    Article Title: The effect of Enterococcus faecium L6 and Lactobacillus plantarum L10 combination on broiler growth and protection against enterohemorrhagic Escherichia coli O157:H7 challenge
    Article Snippet: .. Total RNA was extracted from jejunum with the High Purity RNA Tissue Kit (Takara, Japan) followed by purity assessment (OD260/280) on a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). cDNA was subsequently synthesized using the Reverse Transcriptase M-MLV Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was performed on a 7500 Real Time PCR system (Applied Biosystem, USA) with β-actin as the internal control. ..

    Real-time Polymerase Chain Reaction:

    Article Title: The effect of Enterococcus faecium L6 and Lactobacillus plantarum L10 combination on broiler growth and protection against enterohemorrhagic Escherichia coli O157:H7 challenge
    Article Snippet: .. Total RNA was extracted from jejunum with the High Purity RNA Tissue Kit (Takara, Japan) followed by purity assessment (OD260/280) on a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). cDNA was subsequently synthesized using the Reverse Transcriptase M-MLV Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was performed on a 7500 Real Time PCR system (Applied Biosystem, USA) with β-actin as the internal control. ..

    Control:

    Article Title: The effect of Enterococcus faecium L6 and Lactobacillus plantarum L10 combination on broiler growth and protection against enterohemorrhagic Escherichia coli O157:H7 challenge
    Article Snippet: .. Total RNA was extracted from jejunum with the High Purity RNA Tissue Kit (Takara, Japan) followed by purity assessment (OD260/280) on a Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). cDNA was subsequently synthesized using the Reverse Transcriptase M-MLV Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was performed on a 7500 Real Time PCR system (Applied Biosystem, USA) with β-actin as the internal control. ..

    Polymerase Chain Reaction:

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Amplification:

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..



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