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primescript rt  (TaKaRa)


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    Structured Review

    TaKaRa primescript rt
    Primescript Rt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4943 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase/PrimeScript+Reverse+Transcriptase/pm42831675-132-11-13
    Average 97 stars, based on 4943 article reviews
    primescript rt - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: CX3CL1–CX3CR1 axis promotes restraint stress-induced thymic involution through macrophage-mediated thymocyte apoptosis
    Article Snippet: .. Total RNAs were reverse transcribed to complementary DNA (cDNA) using Prime Script Reverse Transcriptase (Takara Bio, Shiga, Japan) with oligo(dT) 15 primers. .. PCR was carried out using SYBR Premix Ex Taq II (Takara Bio) with specific primer sets (Takara Bio, ).

    Article Title: Tumor-Infiltrating mregDCs Restrain Anti-Tumor Immunity in Early Relapse HCC.
    Article Snippet: Cellular RNA was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. Complementary DNA synthesis was carried out using the PrimeScriptTM reverse transcriptase reagent kit (Takara Bio, Inc.). .. Amplification and quantification were conducted using the ABI PRISM 7900 Sequence Detection System (Applied Biosystems; Thermo Fisher Scientific, Inc.) and SYBR Premix Ex TaqTM (Tli RNaseH Plus; Takara Bio, Inc.).

    Article Title: Lactiplantibacillus plantarum HOKKAIDO strain drives alveolar macrophage-dependent antiviral protection against respiratory infection.
    Article Snippet: Total RNA was extracted from the spleen cells using the Direct-zol RNA Miniprep Plus (Zyomo Research, Irvine, CA, USA) and purified using the Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturers’ instructions. .. cDNA was synthesized using PrimeScript Reverse Transcriptase (Takara Bio, Shiga, Japan) with an Oligo dT primer, following the manufacturer’s instructions. ..

    Article Title: XIAOSHUI formula inhibits malignant pleural effusion by targeting the STC1/p65/CXCL5 axis to reprogram tumor-associated macrophages.
    Article Snippet: Background: Malignant pleural effusion (MPE) is a common and debilitating complication in advanced lung carcinoma, driven by an immunosuppressive tumor microenvironment (TME), where tumor-associated macrophages (TAMs) play a pivotal role.. This specialized milieu not only facilitates neoplastic proliferation but also contributes to immune system dysfunction.. Preliminary evidence suggests that Xiaoshui Formula (XSF) could inhibit MPE by modulating TAM polarization, a key mechanism underlying T cell dysfunction and compromised tumor surveillance capabilities.

    Article Title: Repurposing approved drugs as Pendrin ( SLC26A4 ) inhibitors in allergic asthma: single-cell nomination, structure-based screening and functional validation
    Article Snippet: .. Total RNA was extracted with TRIzol reagent and reverse-transcribed with PrimeScript reverse transcriptase (Takara). ..

    Article Title: ACLY promotes epidermal regeneration and mitochondrial homeostasis in burn injury by reducing oxidative stress
    Article Snippet: The RNA quality was measured by NanoDrop spectrophotometry (Thermo Fisher), and only samples with A260/A280 ratios between 1.8 and 2.0 were used for further analysis. .. First-strand cDNA synthesis was performed with PrimeScript reverse transcriptase (Takara). .. Quantitative PCR was conducted using TB Green chemistry (Takara) on StepOnePlus (Applied Biosystems) or CFX96 (Bio-Rad) instruments.

    Article Title: Multi-omic profiling defines subtype-specific extracellular vesicle signatures in early human pregnancy.
    Article Snippet: Small RNA libraries were prepared using a modified SMARTer smRNA-Seq Kit for Illumina (Takara Bio USA, San Jose, CA) optimized for low-input small RNA species including miRNA, small interfering RNA (siRNA), PIWI-interacting RNA (piRNA), transfer RNA (tRNA), and small nucleolar RNA (snoRNA). .. RNA from EVs was polyadenylated and reverse transcribed into cDNA using PrimeScript RT (Takara Bio USA, San Jose, CA) with template switching to incorporate adapter sequences. ..

    Article Title: Differential Expression and Function of Arginase in Mouse Uterus During Early Pregnancy
    Article Snippet: .. Total RNAs were extracted from mouse stromal cells using TRIzol (AG21101, Accurate Biology, Changsha, China), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI, USA), and reverse-transcribed into cDNA with the Prime Script Reverse Transcriptase Reagent Kit (Takara, Japan). .. RT-qPCR was performed using the SYBR Premix (Q311-02-AA, Vazyme, Nanjing, China).

    DNA Synthesis:

    Article Title: Tumor-Infiltrating mregDCs Restrain Anti-Tumor Immunity in Early Relapse HCC.
    Article Snippet: Cellular RNA was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. Complementary DNA synthesis was carried out using the PrimeScriptTM reverse transcriptase reagent kit (Takara Bio, Inc.). .. Amplification and quantification were conducted using the ABI PRISM 7900 Sequence Detection System (Applied Biosystems; Thermo Fisher Scientific, Inc.) and SYBR Premix Ex TaqTM (Tli RNaseH Plus; Takara Bio, Inc.).

    Synthesized:

    Article Title: Lactiplantibacillus plantarum HOKKAIDO strain drives alveolar macrophage-dependent antiviral protection against respiratory infection.
    Article Snippet: Total RNA was extracted from the spleen cells using the Direct-zol RNA Miniprep Plus (Zyomo Research, Irvine, CA, USA) and purified using the Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturers’ instructions. .. cDNA was synthesized using PrimeScript Reverse Transcriptase (Takara Bio, Shiga, Japan) with an Oligo dT primer, following the manufacturer’s instructions. ..

    cDNA Synthesis:

    Article Title: XIAOSHUI formula inhibits malignant pleural effusion by targeting the STC1/p65/CXCL5 axis to reprogram tumor-associated macrophages.
    Article Snippet: Background: Malignant pleural effusion (MPE) is a common and debilitating complication in advanced lung carcinoma, driven by an immunosuppressive tumor microenvironment (TME), where tumor-associated macrophages (TAMs) play a pivotal role.. This specialized milieu not only facilitates neoplastic proliferation but also contributes to immune system dysfunction.. Preliminary evidence suggests that Xiaoshui Formula (XSF) could inhibit MPE by modulating TAM polarization, a key mechanism underlying T cell dysfunction and compromised tumor surveillance capabilities.

    Article Title: ACLY promotes epidermal regeneration and mitochondrial homeostasis in burn injury by reducing oxidative stress
    Article Snippet: The RNA quality was measured by NanoDrop spectrophotometry (Thermo Fisher), and only samples with A260/A280 ratios between 1.8 and 2.0 were used for further analysis. .. First-strand cDNA synthesis was performed with PrimeScript reverse transcriptase (Takara). .. Quantitative PCR was conducted using TB Green chemistry (Takara) on StepOnePlus (Applied Biosystems) or CFX96 (Bio-Rad) instruments.



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