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Shanghai GenePharma reverse transcriptase kit
Reverse Transcriptase Kit, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+kit/reverse+transcriptase+kit/pm29257264-66-16-19
Average 90 stars, based on 1 article reviews
reverse transcriptase kit - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: A tRF ‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT‐PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF ‐16 Inhibits Lung Cancer Progression by Hindering the N6 ‐Methyladenosine Modification of CPT1A mRNA
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single‐end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT‐PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: Osteoclast-derived exosomal miR-30a-3p promotes lead exposure-induced osteoporosis by triggering osteoblastic pyroptosis
Article Snippet: .. To evaluate miRNA expression, complementary DNA was reverse-transcribed according to the cDNA kit manufacturer’s instructions (GenePharma, China). .. Gene products were then amplified with an ABI-Prism 7500 Real-Time PCR System (Applied Biosystems, U.S.A.) using a SYBR Real-Time PCR kit (GenePharma, China).

Article Title: Molecular Mechanism of the Asarum-Angelica Drug Pair in the Treatment of Periodontitis Based on Network Pharmacology and Experimental Verification.
Article Snippet: .. Total RNA from cells was extracted according to the entire RNA extraction kit instructions (GenePharma, Shanghai, China) to remove genomic DNA, with the procedure carried out according to the teachings of the reverse transcription kit and RT-PCR kit (GenePharma, Shanghai, China). ..

Article Title: A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1.
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT-PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF-16 Inhibits Lung Cancer Progression by Hindering the N6-Methyladenosine Modification of CPT1A mRNA.
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single- end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT- PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

SYBR Green Assay:

Article Title: A tRF ‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT‐PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF ‐16 Inhibits Lung Cancer Progression by Hindering the N6 ‐Methyladenosine Modification of CPT1A mRNA
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single‐end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT‐PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1.
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT-PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF-16 Inhibits Lung Cancer Progression by Hindering the N6-Methyladenosine Modification of CPT1A mRNA.
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single- end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT- PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Fluorescence:

Article Title: A tRF ‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT‐PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF ‐16 Inhibits Lung Cancer Progression by Hindering the N6 ‐Methyladenosine Modification of CPT1A mRNA
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single‐end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT‐PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1.
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT-PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF-16 Inhibits Lung Cancer Progression by Hindering the N6-Methyladenosine Modification of CPT1A mRNA.
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single- end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT- PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Real-time Polymerase Chain Reaction:

Article Title: A tRF ‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT‐PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF ‐16 Inhibits Lung Cancer Progression by Hindering the N6 ‐Methyladenosine Modification of CPT1A mRNA
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single‐end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT‐PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1.
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT-PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF-16 Inhibits Lung Cancer Progression by Hindering the N6-Methyladenosine Modification of CPT1A mRNA.
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single- end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT- PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Expressing:

Article Title: A tRF ‐5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT‐PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF ‐16 Inhibits Lung Cancer Progression by Hindering the N6 ‐Methyladenosine Modification of CPT1A mRNA
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single‐end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT‐PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: Osteoclast-derived exosomal miR-30a-3p promotes lead exposure-induced osteoporosis by triggering osteoblastic pyroptosis
Article Snippet: .. To evaluate miRNA expression, complementary DNA was reverse-transcribed according to the cDNA kit manufacturer’s instructions (GenePharma, China). .. Gene products were then amplified with an ABI-Prism 7500 Real-Time PCR System (Applied Biosystems, U.S.A.) using a SYBR Real-Time PCR kit (GenePharma, China).

Article Title: A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1.
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT-PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF-16 Inhibits Lung Cancer Progression by Hindering the N6-Methyladenosine Modification of CPT1A mRNA.
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single- end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT- PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

RNA Extraction:

Article Title: Molecular Mechanism of the Asarum-Angelica Drug Pair in the Treatment of Periodontitis Based on Network Pharmacology and Experimental Verification.
Article Snippet: .. Total RNA from cells was extracted according to the entire RNA extraction kit instructions (GenePharma, Shanghai, China) to remove genomic DNA, with the procedure carried out according to the teachings of the reverse transcription kit and RT-PCR kit (GenePharma, Shanghai, China). ..

Article Title: TRIB3 is a biomarker of poor prognosis in laryngeal squamous cell carcinoma and may affect tumor development through PI3K / AKT / mTOR pathway.
Article Snippet: .. Cells including Tu-212, Tu-686, Tu-177, and 16HBE cells were bought from iCell Bioscience Inc, Shanghai. sh-TRIB13, reverse transcription kit and total RNA extraction kit were from Shanghai Genepharma Co., Ltd. RPMI-1640 and trypsin were from Grand Island Biological Company (USA) and Materigel were from TianHang Biotech Co., Ltd (Zhejiang, China). .. Lipofectarn 2000 (Thermo Scientific, USA), the primary antibody against TRIB3 (Affinity, USA), primary antibodies against GAPDH, PI3K, AKT and mTOR and rabbit secondary antibody (Abbkine Co., Ltd, USA)were also used in this research.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Molecular Mechanism of the Asarum-Angelica Drug Pair in the Treatment of Periodontitis Based on Network Pharmacology and Experimental Verification.
Article Snippet: .. Total RNA from cells was extracted according to the entire RNA extraction kit instructions (GenePharma, Shanghai, China) to remove genomic DNA, with the procedure carried out according to the teachings of the reverse transcription kit and RT-PCR kit (GenePharma, Shanghai, China). ..

Quantitative RT-PCR:

Article Title: A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1.
Article Snippet: .. As per the manufacturer's guide, whole RNA from SW620/R and HCT116/R cells was acquired with the help of a Trizol reagent (Ambion). cDNA was prepared with the help of a reverse transcription system kit (Genepharma). qRT-PCR was carried out using SYBR Green (Genepharma), and the GAPDH was kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Article Title: TRF-16 Inhibits Lung Cancer Progression by Hindering the N6-Methyladenosine Modification of CPT1A mRNA.
Article Snippet: Standard small RNA sequencing was carried out using the Illumina NextSeq platform with 50 bp single- end sequencing. .. According to the manufacturer's instructions, the whole RNA from A549/R and H1299/R cells was extracted using a Trizol reagent (Ambion, USA). cDNA was synthesised using a reverse transcription system kit from Genepharma. qRT- PCR was carried out using SYBR Green (Genepharma, China), and the ACTIN and NAT10 were kept as the endogenous reference on an Agilent Mx3005P fluorescence quantitative PCR instrument to assess the mRNA's relative expression. ..

Polymerase Chain Reaction:

Article Title: Antiliver Fibrosis Formula of Fuzheng Huayu Alleviates Inflammatory Response
Article Snippet: LPS, 1400W, Griess reagent, and MTT were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. MicroRNA primers, transcription kits, and universal PCR master mix were obtained from GenePharma Company (Shanghai, China). ..



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