primescripttm reverse transcriptase reagent kit (TaKaRa)
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primescripttm reverse transcriptase reagent kit
Primescripttm Reverse Transcriptase Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+kit/PrimeScript+Reverse+Transcriptase/pm42133204-232-8-13
Average 97 stars, based on 4907 article reviews
Primescripttm Reverse Transcriptase Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+kit/PrimeScript+Reverse+Transcriptase/pm42133204-232-8-13
Average 97 stars, based on 4907 article reviews
primescripttm reverse transcriptase reagent kit - by Bioz Stars,
2026-09
97/100 stars
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Reverse Transcription:Article Title: Comprehensive genome-wide identification, structural analysis, and cold-responsive expression profiling of double B-box (DBB) transcription factors in melon (Cucumis melo L.). Article Snippet: .. Total RNA was extracted from melon leaves with the help of RNase inhibitor (Thermo Fisher Scientific, AM2696) and DNA/RNA-free water (Tiangen Biotech (Beijing) Co., Ltd., RT121), and then reverse transcribed using Article Title: Gel-free library preparation for next-generation RNA sequencing and small RNA quantification. Article Snippet: Excess Linker 1 not ligated to RNAs was then deadenylated using 5’-deadenylase (New England Biolabs) through incubation at 30 °C for 1 h and subsequently digested with RecJf exonuclease (New England Biolabs) through two 30-min incubations at 37 °C. .. Reverse transcription (RT) was initiated with a 2-min incubation at 80 °C to anneal the RT primers to the RNA template, followed by a 2-h incubation at 50 °C to synthesize the cDNA strand using the Article Title: Tumor-Infiltrating mregDCs Restrain Anti-Tumor Immunity in Early Relapse HCC. Article Snippet: Cellular RNA was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. Complementary DNA synthesis was carried out using the Article Title: Dendrobine promotes osteogenesis-angiogenesis in postmenopausal osteoporosis by inhibiting Hippo signaling pathway. Article Snippet: 3 Wuhan Fourth Hospital, No.473 Hanzheng Street, Wuhan, Hubei, China Abstract Postmenopausal osteoporosis (PMOP) is a skeletal disorder marked by progressive bone mineral density decline and increased susceptibility to fractures.. Accumulating evidence indicates that coupled osteogenesis and angiogenesis are indispensable for bone remodeling and repair.. This study investigated the therapeutic potential of dendrobine in concurrently modulating osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and angiogenic activity in the context of PMOP. Article Title: The astrocyte-enriched gene Tmem44 regulates circadian protein translation in mouse astrocytes Article Snippet: Total RNA was extracted from cells and purified using the RNeasy Plus Micro Kit (Qiagen, Germany, 74034). .. 1 μg of total RNA was reverse transcribed using an oligo-dT primer and PrimeScript RTase (TaKaRa (Japan), Article Title: SARS-CoV-2 3CLpro mutations T21I and E166A confer differential resistance to simnotrelvir, bofutrelvir, and ensitrelvir. Article Snippet: .. Viral RNA from the lung tissues was extracted using the RNeasy Mini Kit (Qiagen) and Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways Article Snippet: .. Extraction of total RNA from cultured cells, RT and qPCR were performed using the NucleoSpin RNA kit, Article Title: Differential Expression and Function of Arginase in Mouse Uterus During Early Pregnancy Article Snippet: .. Total RNAs were extracted from mouse stromal cells using TRIzol (AG21101, Accurate Biology, Changsha, China), digested with RQ1 deoxyribonuclease I (Promega, Fitchburg, WI, USA), and reverse-transcribed into cDNA with the Incubation:Article Title: Gel-free library preparation for next-generation RNA sequencing and small RNA quantification. Article Snippet: Excess Linker 1 not ligated to RNAs was then deadenylated using 5’-deadenylase (New England Biolabs) through incubation at 30 °C for 1 h and subsequently digested with RecJf exonuclease (New England Biolabs) through two 30-min incubations at 37 °C. .. Reverse transcription (RT) was initiated with a 2-min incubation at 80 °C to anneal the RT primers to the RNA template, followed by a 2-h incubation at 50 °C to synthesize the cDNA strand using the DNA Synthesis:Article Title: Tumor-Infiltrating mregDCs Restrain Anti-Tumor Immunity in Early Relapse HCC. Article Snippet: Cellular RNA was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. Complementary DNA synthesis was carried out using the Fluorescence:Article Title: SARS-CoV-2 3CLpro mutations T21I and E166A confer differential resistance to simnotrelvir, bofutrelvir, and ensitrelvir. Article Snippet: .. Viral RNA from the lung tissues was extracted using the RNeasy Mini Kit (Qiagen) and Real-time Polymerase Chain Reaction:Article Title: SARS-CoV-2 3CLpro mutations T21I and E166A confer differential resistance to simnotrelvir, bofutrelvir, and ensitrelvir. Article Snippet: .. Viral RNA from the lung tissues was extracted using the RNeasy Mini Kit (Qiagen) and Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways Article Snippet: .. Extraction of total RNA from cultured cells, RT and qPCR were performed using the NucleoSpin RNA kit, Extraction:Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways Article Snippet: .. Extraction of total RNA from cultured cells, RT and qPCR were performed using the NucleoSpin RNA kit, Cell Culture:Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways Article Snippet: .. Extraction of total RNA from cultured cells, RT and qPCR were performed using the NucleoSpin RNA kit, |