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iscript reverse transcriptase 198  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript reverse transcriptase 198
    Iscript Reverse Transcriptase 198, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+kit/IScript+C+DNA+Synthesis+Kit/pm42095486-85-13-17
    Average 99 stars, based on 6773 article reviews
    iscript reverse transcriptase 198 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    Article Title: Human induced pluripotent stem cell-derived inner ear organoids reveal hair cell damage and plasticity after cisplatin and gentamicin exposure
    Article Snippet: RNA concentration was measured using NanoDrop One (Thermo Fisher Scientific), and samples were stored at −80°C. .. For the generation of cDNA, a reaction mixture was made consisting of iScript reaction mix (iScript kit, 1708891, Bio-Rad), the iScript reverse transcriptase, nuclease-free water and the RNA template (500 ng), according to the iScript manual. .. Thermal cycling (Bio-Rad S1000 Thermal Cycler) occurred by priming for 5 min at 25°C, reverse transcription at 46°C for 20 min, reverse transcriptase inactivation for 1 min at 95°C and cooling at 4°C.

    Article Title: Octopamine regulates neural circuits in the mushroom body and central complex, influencing sleep and arousal
    Article Snippet: Three independent sets of 30 heads were ground using a pestle (Kimble Disposable pellet pestle) and RNA was isolated using QIAGen RNeasy kit based on manufacturer instructions. .. 14 μL of total RNA from each sample was reverse transcribed with the iScript cDNA Synthesis Kits (BIO-RAD Inc). ..

    Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation
    Article Snippet: .. Reverse transcription for ligation junction accuracy used the iScript kit (Bio-Rad #1708 891) containing random hexamers. ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Isolation:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    DNA Synthesis:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    Polymerase Chain Reaction:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    Purification:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Synthesized:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    cDNA Synthesis:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Article Title: Octopamine regulates neural circuits in the mushroom body and central complex, influencing sleep and arousal
    Article Snippet: Three independent sets of 30 heads were ground using a pestle (Kimble Disposable pellet pestle) and RNA was isolated using QIAGen RNeasy kit based on manufacturer instructions. .. 14 μL of total RNA from each sample was reverse transcribed with the iScript cDNA Synthesis Kits (BIO-RAD Inc). ..

    Article Title: Integrated multiomics reveals starvation-driven keratin degradation and persistence in Fervidobacterium islandicum AW-1
    Article Snippet: CPM (7-diethylamino-3-(4’-maleimidylphenyl)-4-methyl-coumarin) , Invitrogen, MA, USA , Cat# D346. .. iScript cDNA Synthesis Kit , Bio-Rad, CA, USA , Cat# 1708890. .. 2× SsoAdvancedTM Universal SYBR Green Supermix , Bio-Rad, CA, USA , Cat# 1725271.

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. RNA was stored at − 80◦C for subsequent cDNA synthesis, which was carried out within 24 h using the iScript cDNA Synthesis Kit (Bio-Rad, 1708890). ..

    Quantitative RT-PCR:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    SYBR Green Assay:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Ligation:

    Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation
    Article Snippet: .. Reverse transcription for ligation junction accuracy used the iScript kit (Bio-Rad #1708 891) containing random hexamers. ..



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