polymorphprep tm (lot: 00121) (Axis-Shield Diagnostics)

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Axis-Shield Diagnostics polymorphprep tm (lot: 00121)

Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

Polymorphprep Tm (Lot: 00121), supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+tm/polymorphprep/pmc11719704-155-0-7
Average 90 stars, based on 1 article reviews

polymorphprep tm (lot: 00121) - by Bioz Stars, 2026-11

90/100 stars

Images

1) Product Images from "Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways"

Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms26010143

Figure Legend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

Techniques Used: Extraction, Isolation

Isolation:

Article Title: Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection
Article Snippet: .. Neutrophils were isolated using PolymorphPrep (Axis-Shield). ..

Article Title: Immune complexes-mediated activation of neutrophils in systemic lupus erythematosus is dependent on RNA recognition by toll-like receptor 8
Article Snippet: .. Neutrophils were isolated from healthy subjects with Polymorphprep (Axis-Shield, Dundee, UK) as described previously ( , ). .. Neutrophils were isolated from healthy subjects with Polymorphprep (Axis-Shield, Dundee, UK) as described previously ( , ).

Article Title: The C-X-C Motif Chemokine Ligand 5, Which Exerts an Antioxidant Role by Inducing HO-1 Expression, Is C-X-C Motif Chemokine Receptor 2-Dependent in Human Prostate Stroma and Cancer Cells
Article Snippet: .. Neutrophils drawn from subjects’ peripheral blood were isolated by PolymorphPrep TM (Axis-Shield PoC, Oslo, Norway) provided by Dr. I-Hsien Lin, Department of Microbiology and Immunology, Chang Gung University, Taiwan. .. Neutrophils (1.5 × 10 6 ) were suspended in a 200 μL serum-free medium and added to the upper chamber of the transwell, with a pore size of 3.0 μm (PITP01250, Merck Millipore Ltd., Darmstadt, Germany).

other:

Article Title: Simvastatin ameliorates adverse pregnancy by inhibiting glycolysis-related NETs in obstetrical antiphospholipid syndrome.
Article Snippet: Fresh venous blood was drawn from normal pregnant women aged between 22 and 35 at 10–12 weeks of gestation, spread gently on PolymorphprepTM (Axis-Shield, Dundee, Scotland), and centrifuged at 750g for 35 min at room temperature.

Article Title: Extracellular traps in peripheral blood mononuclear cell fraction in childhood-onset systemic lupus erythematosus.
Article Snippet: Briefly, blood samples were mixed with Ficoll-Paque (Robbins Scientific Corporation, Sunnyvale, CA, USA) and PolymorphprepTM (Axis-Shield, Oslo, Norway) in a 1:1:1 ratio in a 50 mL-sterile tube and centrifuged at 1000 ×g for 30 min at room temperature without brake.

Article Title: Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma.
Article Snippet: Neutrophils were isolated from peripheral blood using PolymorphprepTM (AS1114683, Axis-Shield, Norway) density gradient centrifugation.

Immunology:

Saline:

Article Title: Whole-blood model reveals granulocytes as key sites of dengue virus propagation, expanding understanding of disease pathogenesis
Article Snippet: .. Packed cells remaining after PRP removal were diluted 1:1 with normal saline and centrifuged on Polymorphprep (Axis-Shield, Dundee, UK) to separate leukocytes from RBCs. .. Leukocytes were washed and stained with CD markers for sorting by FACSAria III (BD Biosciences, NJ, USA).

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