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polymorphprep tm  (Progen Biotechnik)

 
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    Progen Biotechnik polymorphprep tm
    Polymorphprep Tm, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polymorphprep+tm/polymorphprep/pmc11501092-70-16-18
    Average 90 stars, based on 1 article reviews
    polymorphprep tm - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Functional classification of antineutrophil cytoplasmic antibody (ANCA) and its relation with clinical parameters of ANCA-associated vasculitis
    Article Snippet: .. Neutrophils were isolated from the peripheral blood of healthy volunteers using Polymorphprep (Progen, Heidelberg, Germany) and suspended in RPMI 1640 with 10 % fetal bovine serum (1.0 × 10 /mL). ..

    Article Title: Advanced glycation end product (AGE)-modified albumin does not enhance neutrophil extracellular trap formation and minimally impacts endothelial inflammation in large vessels.
    Article Snippet: Aims: Advanced glycation end products (AGEs), particularly AGE-modified albumin (AGE-BSA), have been implicated in vascular inflammation and endothelial dysfunction, while neutrophil extracellular traps (NETs) are known to aggravate endothelial injury.. Here, we investigated whether AGE-BSA directly promotes endothelial inflammatory and thrombotic activation, modulates neutrophil oxidative stress and NET formation, and may enhance NET-induced endothelial inflammation.. Materials, methods, and key findings: In human aortic and human umbilical vein endothelial cells, two different endotoxin-free AGE-BSA formulations did not or only minimally alter the expression or secretion of inflammatory (IL-6, IL-1β, CCL2) or thrombotic (VCAM-1, tissue factor) markers.

    Article Title: Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancers.
    Article Snippet: .. We isolated human peripheral blood mononuclear cells (PBMCs) from whole blood samples of healthy donors by density gradient centrifugation using Polymorphprep (Progen), according to the manufacturer’s protocol. .. NK cells were purified from PBMCs by MACS-based negative selection using the MojoSort Human NK Cell Isolation Kit (BioLegend) according to the manufacturer’s instructions.

    Gradient Centrifugation:

    Article Title: Advanced glycation end product (AGE)-modified albumin does not enhance neutrophil extracellular trap formation and minimally impacts endothelial inflammation in large vessels.
    Article Snippet: Aims: Advanced glycation end products (AGEs), particularly AGE-modified albumin (AGE-BSA), have been implicated in vascular inflammation and endothelial dysfunction, while neutrophil extracellular traps (NETs) are known to aggravate endothelial injury.. Here, we investigated whether AGE-BSA directly promotes endothelial inflammatory and thrombotic activation, modulates neutrophil oxidative stress and NET formation, and may enhance NET-induced endothelial inflammation.. Materials, methods, and key findings: In human aortic and human umbilical vein endothelial cells, two different endotoxin-free AGE-BSA formulations did not or only minimally alter the expression or secretion of inflammatory (IL-6, IL-1β, CCL2) or thrombotic (VCAM-1, tissue factor) markers.

    Article Title: Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancers.
    Article Snippet: .. We isolated human peripheral blood mononuclear cells (PBMCs) from whole blood samples of healthy donors by density gradient centrifugation using Polymorphprep (Progen), according to the manufacturer’s protocol. .. NK cells were purified from PBMCs by MACS-based negative selection using the MojoSort Human NK Cell Isolation Kit (BioLegend) according to the manufacturer’s instructions.



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    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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    polymorphprep tm  (Progen Biotechnik)
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    Progen Biotechnik polymorphprep tm
    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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    Image Search Results


    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Journal: International Journal of Molecular Sciences

    Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

    doi: 10.3390/ijms26010143

    Figure Lengend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Article Snippet: PolymorphPrep TM (Lot: 00121) was purchased from Axis-Shield (Serumwerk Bernburg AG, Bernburg, Germany).

    Techniques: Extraction, Isolation