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chloramphenicol transferase (cat) reporter plasmid pcat-basic  (Promega)

 
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    Structured Review

    Promega chloramphenicol transferase (cat) reporter plasmid pcat-basic
    Chloramphenicol Transferase (Cat) Reporter Plasmid Pcat Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcat-basic/chloramphenicol+acetyltransferase+vector+pcat+basic+reporter+gene/pm11313983-135-31-42
    Average 90 stars, based on 1 article reviews
    chloramphenicol transferase (cat) reporter plasmid pcat-basic - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Silencer Elements Modulate the Expression of the Gene for the Neuron-Glia Cell Adhesion Molecule, Ng-CAM
    Article Snippet: Construction of Reporter Plasmids—Reporter gene plasmids were constructed in the promoterless chloramphenicol acetyltransferase (CAT) gene vector pCAT-basic (Promega, Madison, WI) and pBluescript-II (Stratagene, La Jolla, CA) and are shown schematically in Fig. 3.

    Article Title: Interferon-γ Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1α to a Distinct Interferon-γ-responsive Element
    Article Snippet: Preparation of CAT Reporter Gene Constructs—The pECAT clone contains approximately 1800 bp of 59-flanking sequence and was generated by sequentially subcloning HindIII-PstI (21800 to 2174) and PstI-AlwNI (2174 to 12) promoter fragments from the HLA 6.2 genomic clone (kindly provided by Harry Orr (50)) into the HindIII and PstI sites of the promoterless chloramphenicol acetyltransferase (CAT) reporter gene vector pCAT-Basic (Promega).

    Article Title: Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus.
    Article Snippet: Construction of reporter plasmids containing DNA sequences from the putative vIRF promoter region Various lengths of DNA fragments upstream of the vIRF translational initiating ATG codon were PCR-ampli®ed and inserted into chloramphenicol transferase (CAT) reporter plasmid pCAT-Basic, a promoterless and enhancerless vector (Promega), in a sense orientation relative to the CAT coding sequence at HindIII and XbaI sites.

    Article Title: Control of Human Muscle-type Carnitine Palmitoyltransferase I Gene Transcription by Peroxisome Proliferator-activated Receptor
    Article Snippet: The PstI-AvrII-digested PCR product was cloned into the PstI-XbaI sites of chloramphenicol acetyltransferase (CAT) vector pCAT-BASIC reporter gene (Promega).

    Control:

    Article Title: Dual control of glut1 glucose transporter gene expression by hypoxia and by inhibition of oxidative phosphorylation.
    Article Snippet: Nonspecific plasmid DNA (pBluescript, Stratagene) was mixed with the smaller test vectors to adjust to a total of 10 mg of DNA. .. To control for the efficiency of transfection, 10 mg of a chloramphenicol acetyltransferase reporter construct containing an SV40 promoter/enhancer (pCAT-Control, Promega) was also cotransfected. ..

    Transfection:

    Article Title: Dual control of glut1 glucose transporter gene expression by hypoxia and by inhibition of oxidative phosphorylation.
    Article Snippet: Nonspecific plasmid DNA (pBluescript, Stratagene) was mixed with the smaller test vectors to adjust to a total of 10 mg of DNA. .. To control for the efficiency of transfection, 10 mg of a chloramphenicol acetyltransferase reporter construct containing an SV40 promoter/enhancer (pCAT-Control, Promega) was also cotransfected. ..

    Construct:

    Article Title: Dual control of glut1 glucose transporter gene expression by hypoxia and by inhibition of oxidative phosphorylation.
    Article Snippet: Nonspecific plasmid DNA (pBluescript, Stratagene) was mixed with the smaller test vectors to adjust to a total of 10 mg of DNA. .. To control for the efficiency of transfection, 10 mg of a chloramphenicol acetyltransferase reporter construct containing an SV40 promoter/enhancer (pCAT-Control, Promega) was also cotransfected. ..



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    Promega pcat basic
    Relative enhancer activities of fragments of <t>HcTnC</t> <t>intron</t> 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into <t>pCAT</t> Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.
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    The  CAT  expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of  CAT  reporter plasmids

    Journal: Virology Journal

    Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

    doi: 10.1186/1743-422X-6-212

    Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

    Techniques: Expressing, Transfection

    Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Journal: Gene Expression

    Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

    doi:

    Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

    Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control