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cat-reporter gene pcatbasic  (Promega)

 
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    Promega cat-reporter gene pcatbasic
    Cat Reporter Gene Pcatbasic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcat-basic/cat+reporter+gene+pcatbasic/pm09927313-96-22-25
    Average 90 stars, based on 1 article reviews
    cat-reporter gene pcatbasic - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: USF2 inhibits C/EBP-mediated transcriptional regulation of the RIIβ subunit of cAMP-dependent protein kinase
    Article Snippet: A reporter construct containing the basal promoter of the rat RIIβ 5'-flanking region (-394 to -123) upstream of a CAT-reporter gene (pCATbasic; Promega, Madison, WI) was used for assaying RIIβ promoter activity [ ].

    Article Title: Isoform-specific regulation of the CCAAT/enhancer-binding protein family of transcription factors by 3',5'-cyclic adenosine monophosphate in Sertoli cells.
    Article Snippet: A construct containing the basal promoter and cAMP-responsive region of the rat RIIb 59-flanking region (2723 to 2123) in front of a CAT-reporter gene (pCATbasic; Promega Corp.) (20) and a construct containing the cAMP-responsive region of the rat phosphodiesterase (PDE) 4D1/2 59-flanking region (21540 to 12) in front of a luciferase reporter gene (pGL2Basic; Promega Corp.) (21) were cotransfected with either the CMV-C/EBP b (NF-IL6) expression vector (22) or the CMVcontaining pCRTM3 vector (Invitrogen Corp., San Diego, CA).

    Transfection:

    Article Title: Method of transfection affects the cAMP-mediated induction of the RIIbeta subunit of protein kinase A in Sertoli cells: inhibition of response by increase in intracellullar calcium.
    Article Snippet: .. The basal promoter and cAMP-responsive region of the rat RIIb 50-flanking region (1723 to 1123) in front of a CAT reporter gene (pCATbasic; Promega, Madison, WI, USA) in forward (RIIbCAT) and reverse (revRIIbCAT) orientation (14) were used for transfections. ..



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    The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Journal: Virology Journal

    Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

    doi: 10.1186/1743-422X-6-212

    Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

    Techniques: Expressing, Transfection

    Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Journal: Gene Expression

    Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

    doi:

    Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

    Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control