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chloramphenicol acetyltransferase (cat) gene reporter plasmid (pcat-basic)  (Promega)

 
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    Promega chloramphenicol acetyltransferase (cat) gene reporter plasmid (pcat-basic)
    Chloramphenicol Acetyltransferase (Cat) Gene Reporter Plasmid (Pcat Basic), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcat-basic/chloramphenicol+acetyltransferase++cat++gene+reporter+plasmid++pcat+basic+/pm09760255-19-26-34
    Average 90 stars, based on 1 article reviews
    chloramphenicol acetyltransferase (cat) gene reporter plasmid (pcat-basic) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.

    Sequencing:

    Article Title: Analysis of the TPA regulatory element in the genomic poly(ADP-ribose) synthetase gene in human leukemia U937 cells.
    Article Snippet: The human leukemia U937 cells differentiate into monocyte/macrophage-like cells when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA).. We observed that during this process, both protein and mRNA levels for PARS markedly decreased in U937 cells.. Through deletion analysis of the PARS regulatory gene, we found that the sequence within the first intron region was responsible for the TPAdependent repression.



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    Image Search Results


    The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Journal: Virology Journal

    Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

    doi: 10.1186/1743-422X-6-212

    Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

    Techniques: Expressing, Transfection

    Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Journal: Gene Expression

    Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

    doi:

    Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

    Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control