lambda fixii (Agilent technologies)

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Agilent technologies lambda fixii
Lambda Fixii, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+fixii/us08133498-150-31-36
Average 90 stars, based on 1 article reviews

lambda fixii - by Bioz Stars, 2026-09

90/100 stars

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Knock-Out:

Article Title: STAT3 and Oct-3/4 Control Histone Modification through Induction of Eed in Embryonic Stem Cells
Article Snippet: A portion of lysate (50 g of protein) was subjected to Western blotting with antibodies against Eed (Upstate Biotechnology, Inc., Lake Placid, NY), H3K27triMe (Upstate Biotechnology), lamin B (Santa Cruz Biotechnology, Santa Cruz, CA) andMyc epitope (9E10) (Santa Cruz Biotechnology). .. Establishment of Eed Conditional Knock-out ES Cells—The 15-kb fragment running from 6 kb upstream of the first exon to 0.3 kb downstreamof the fourth exonwas isolated by PCR from a Lambda FIXII library (Stratagene) and subcloned into the SalI site of pBluescript II KS (Stratagene). ..

Isolation:

Polymerase Chain Reaction:

Cloning:

Article Title: Use of polynucleotides encoding small acid-soluble spore protein for inhibiting bacterial cell growth and/or treating bacterial infections
Article Snippet: .. There are two classes of lambda cloning vectors: insertion vectors accept 0-12 kb DNA and include Lambda ZAPII, Uni-ZAP XR, and Lambda ZAP-Express (Stratagene); replacement vectors accept 9-23 kb and include Lambda FIXII and Lambda DASHII (Stratagene). .. Bacterial protein expression kits allowing expression of toxic cenes are also available including the Lambda CE6 bacteriophage carrying the T7 RNA polymerase gene for delivery to E.coli strain BL21 cells (Stratagene).

Article Title: Use of polynucleotides encoding small acid-soluble spore protein for inhibiting bacterial cell growth and/or treating bacterial infections
Article Snippet: .. There are two classes of lambda cloning vectors: insertion vectors accept 0-12 kb DNA and include Lambda ZAPII, Uni-ZAP XR, and Lambda ZAP-Express (Stratagene); replacement vectors accept 9-23 kb and include Lambda FIXII and Lambda DASHII (Stratagene). .. Bacterial protein expression kits allowing expression of toxic genes are also available including the Lambda CE6 bacteriophage carrying the T7 RNA polymerase gene for delivery to E. coli strain BL21 cells (Stratagene).

BAC Assay:

Article Title: The family of DOF transcription factors: from green unicellular algae to vascular plants.
Article Snippet: .. Screening of barley genomic, BAC and EST libraries A 150 bp probe corresponding to the DOF domain of the barley Pbf gene (Mena et al. 1998) was used to screen a Hordeum vulgare cv Morex BAC library and a cv Igri genomic library in Lambda FIXII (Stratagene; Isabel-LaMoneda et al. 2003). ..

Construct:

Article Title: An insertion of oleate desaturase homologous sequence silences via siRNA the functional gene leading to high oleic acid content in sunflower seed oil.
Article Snippet: Classical sunXower varieties display a high linoleic acid content in their seeds [low oleic (LO) varieties] whereas genotypes carrying the Pervenets mutation display an increased oleic acid content of above 83% [high oleic (HO) varieties].. Despite the advantage in health terms of oleic acid, the nature of the mutation was still unknown.. Previous work reported that HO genotypes carried a speciWc oleate desaturase (OD) allele.

Review