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lambda fixii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda fixii vector
    Lambda Fixii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+fixii/pmc01779807-439-21-24
    Average 90 stars, based on 1 article reviews
    lambda fixii vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Targeted deletion of Hsf1, 2, and 4 genes in mice
    Article Snippet: .. A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene was isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Targeted deletion of Hsf1, 2, and 4 genes in mice
    Article Snippet: .. Targeting vector Isolation of the hsf2 gene A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene was isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
    Article Snippet: .. Targeting vector: Isolation of the hsf2 gene: A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene were isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Article Title: Protection against Oxidative Stress-induced Hepatic Injury by Intracellular Type II Platelet-activating Factor Acetylhydrolase by Metabolism of Oxidized Phospholipids in Vivo
    Article Snippet: .. Generation of Pafah2 / Mice—Pafah2 genomic clones were isolated from a mouse 129/SvJ genomic library in the Lambda FIXII vector (Stratagene, La Jolla, CA). ..

    Hybridization:

    Article Title: Targeted deletion of Hsf1, 2, and 4 genes in mice
    Article Snippet: .. A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene was isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Targeted deletion of Hsf1, 2, and 4 genes in mice
    Article Snippet: .. Targeting vector Isolation of the hsf2 gene A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene was isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
    Article Snippet: .. Targeting vector: Isolation of the hsf2 gene: A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene were isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants.
    Article Snippet: A cotton (Gossypium hirsutum L.) genomic clone encompassing a 17.9-kb DNA fragment was found to contain a delta-12 fatty acid desaturase gene (designated FAD2-4).. The FAD2-4 open reading frame has 1,155 bp and is uninterrupted, encoding a conceptual FAD2-4 polypeptide of 384 amino acids that has 98% identity with the cotton FAD2-3 polypeptide.. The FAD2-4 gene has a single intron of 2,780 bp in its 50-untranslated region (50-UTR).

    Plasmid Preparation:

    Article Title: Targeted deletion of Hsf1, 2, and 4 genes in mice
    Article Snippet: .. Targeting vector Isolation of the hsf2 gene A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene was isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Characterization of a RAB5 homologue in Trypanosoma cruzi.
    Article Snippet: RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion.. RABs show a remarkable specificity in subcellular localization, so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas disease.. RAB5 is a component of early endosomes.

    Article Title: Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
    Article Snippet: .. Targeting vector: Isolation of the hsf2 gene: A 22 kb DNA fragment containing 3.8 kb of the promoter region and the first 6 of the 12 exons of the murine hsf2 gene were isolated from a 129/SvJ mouse genomic library in Lambda FixII vector (Stratagene, La Jolla, CA) by hybridization with a mouse hsf2 exon 1 cDNA as a probe. ..

    Article Title: Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants.
    Article Snippet: A cotton (Gossypium hirsutum L.) genomic clone encompassing a 17.9-kb DNA fragment was found to contain a delta-12 fatty acid desaturase gene (designated FAD2-4).. The FAD2-4 open reading frame has 1,155 bp and is uninterrupted, encoding a conceptual FAD2-4 polypeptide of 384 amino acids that has 98% identity with the cotton FAD2-3 polypeptide.. The FAD2-4 gene has a single intron of 2,780 bp in its 50-untranslated region (50-UTR).

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Sequencing:

    Article Title: Characterization of a RAB5 homologue in Trypanosoma cruzi.
    Article Snippet: RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion.. RABs show a remarkable specificity in subcellular localization, so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas disease.. RAB5 is a component of early endosomes.

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Generated:

    Article Title: Characterization of a RAB5 homologue in Trypanosoma cruzi.
    Article Snippet: RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion.. RABs show a remarkable specificity in subcellular localization, so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas disease.. RAB5 is a component of early endosomes.

    cDNA Library Assay:

    Article Title: Characterization of a RAB5 homologue in Trypanosoma cruzi.
    Article Snippet: RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion.. RABs show a remarkable specificity in subcellular localization, so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas disease.. RAB5 is a component of early endosomes.

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Construct:

    Article Title: Characterization of a RAB5 homologue in Trypanosoma cruzi.
    Article Snippet: RAB proteins are small GTPases involved in exocytic and endocytic pathways of eukaryotic cells, controlling vesicle docking and fusion.. RABs show a remarkable specificity in subcellular localization, so they can be used as molecular markers for studying protein trafficking in Trypanosoma cruzi, the causal agent of Chagas disease.. RAB5 is a component of early endosomes.

    Clone Assay:

    Article Title: Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants.
    Article Snippet: A cotton (Gossypium hirsutum L.) genomic clone encompassing a 17.9-kb DNA fragment was found to contain a delta-12 fatty acid desaturase gene (designated FAD2-4).. The FAD2-4 open reading frame has 1,155 bp and is uninterrupted, encoding a conceptual FAD2-4 polypeptide of 384 amino acids that has 98% identity with the cotton FAD2-3 polypeptide.. The FAD2-4 gene has a single intron of 2,780 bp in its 50-untranslated region (50-UTR).

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Article Title: Protection against Oxidative Stress-induced Hepatic Injury by Intracellular Type II Platelet-activating Factor Acetylhydrolase by Metabolism of Oxidized Phospholipids in Vivo
    Article Snippet: .. Generation of Pafah2 / Mice—Pafah2 genomic clones were isolated from a mouse 129/SvJ genomic library in the Lambda FIXII vector (Stratagene, La Jolla, CA). ..

    Ligation:

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Polymerase Chain Reaction:

    Article Title: Pre-Bilaterian Origins of the Hox Cluster and the Hox Code: Evidence from the Sea Anemone, Nematostella vectensis
    Article Snippet: .. Additional experimental confirmation was provided by the following: (1) isolation and sequencing of clones from a genomic library prepared in the Lambda FixII vector (Stratagene), (2) genomic walking using the techniques of ligation-mediated PCR and long PCR, and (3) isolation and sequencing of transcripts obtained by screening a cDNA library or by RACE. ..

    Mouse Assay:

    Article Title: Protection against Oxidative Stress-induced Hepatic Injury by Intracellular Type II Platelet-activating Factor Acetylhydrolase by Metabolism of Oxidized Phospholipids in Vivo
    Article Snippet: .. Generation of Pafah2 / Mice—Pafah2 genomic clones were isolated from a mouse 129/SvJ genomic library in the Lambda FIXII vector (Stratagene, La Jolla, CA). ..



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