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density gradient centrifugation with polymorphprep  (Progen Biotechnik)

 
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    Structured Review

    Progen Biotechnik density gradient centrifugation with polymorphprep
    Density Gradient Centrifugation With Polymorphprep, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/density+gradient+polymorphprep/polymorphprep/pm42142621-52-7-12
    Average 86 stars, based on 1 article reviews
    density gradient centrifugation with polymorphprep - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Functional classification of antineutrophil cytoplasmic antibody (ANCA) and its relation with clinical parameters of ANCA-associated vasculitis
    Article Snippet: .. Neutrophils were isolated from the peripheral blood of healthy volunteers using Polymorphprep (Progen, Heidelberg, Germany) and suspended in RPMI 1640 with 10 % fetal bovine serum (1.0 × 10 /mL). ..

    Article Title: Advanced glycation end product (AGE)-modified albumin does not enhance neutrophil extracellular trap formation and minimally impacts endothelial inflammation in large vessels.
    Article Snippet: Aims: Advanced glycation end products (AGEs), particularly AGE-modified albumin (AGE-BSA), have been implicated in vascular inflammation and endothelial dysfunction, while neutrophil extracellular traps (NETs) are known to aggravate endothelial injury.. Here, we investigated whether AGE-BSA directly promotes endothelial inflammatory and thrombotic activation, modulates neutrophil oxidative stress and NET formation, and may enhance NET-induced endothelial inflammation.. Materials, methods, and key findings: In human aortic and human umbilical vein endothelial cells, two different endotoxin-free AGE-BSA formulations did not or only minimally alter the expression or secretion of inflammatory (IL-6, IL-1β, CCL2) or thrombotic (VCAM-1, tissue factor) markers.

    Article Title: Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancers.
    Article Snippet: .. We isolated human peripheral blood mononuclear cells (PBMCs) from whole blood samples of healthy donors by density gradient centrifugation using Polymorphprep (Progen), according to the manufacturer’s protocol. .. NK cells were purified from PBMCs by MACS-based negative selection using the MojoSort Human NK Cell Isolation Kit (BioLegend) according to the manufacturer’s instructions.

    Gradient Centrifugation:

    Article Title: Advanced glycation end product (AGE)-modified albumin does not enhance neutrophil extracellular trap formation and minimally impacts endothelial inflammation in large vessels.
    Article Snippet: Aims: Advanced glycation end products (AGEs), particularly AGE-modified albumin (AGE-BSA), have been implicated in vascular inflammation and endothelial dysfunction, while neutrophil extracellular traps (NETs) are known to aggravate endothelial injury.. Here, we investigated whether AGE-BSA directly promotes endothelial inflammatory and thrombotic activation, modulates neutrophil oxidative stress and NET formation, and may enhance NET-induced endothelial inflammation.. Materials, methods, and key findings: In human aortic and human umbilical vein endothelial cells, two different endotoxin-free AGE-BSA formulations did not or only minimally alter the expression or secretion of inflammatory (IL-6, IL-1β, CCL2) or thrombotic (VCAM-1, tissue factor) markers.

    Article Title: Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancers.
    Article Snippet: .. We isolated human peripheral blood mononuclear cells (PBMCs) from whole blood samples of healthy donors by density gradient centrifugation using Polymorphprep (Progen), according to the manufacturer’s protocol. .. NK cells were purified from PBMCs by MACS-based negative selection using the MojoSort Human NK Cell Isolation Kit (BioLegend) according to the manufacturer’s instructions.



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