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Axis-Shield Diagnostics polymorphprep™ density gradient media
Polymorphprep™ Density Gradient Media, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+polymorphprep/polymorphprep+density+gradient/pm37311354-46-11-15
Average 90 stars, based on 1 article reviews
polymorphprep™ density gradient media - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: Knockdown of versican 1 in lung fibroblasts aggravates Lipopolysaccharide-induced acute inflammation through up-regulation of the SP1-Toll-like Receptor 2-NF-κB Axis: a potential barrier to promising Versican-targeted therapy.
Article Snippet: Objective: Versican participates in various pathological processes like inflammation and fibrosis and is a potential therapeutic target for inflammatory diseases.. Versican 1 (V1) has increased expression in inflammatory diseases, but its role is unclear.. We explored the effects of V1 on acute lung inflammation to determine whether targeting V1 had therapeutic potential.

Isolation:

Article Title: A20 and the noncanonical NF- κ B pathway are key regulators of neutrophil recruitment during fetal ontogeny
Article Snippet: Differentiation of Hoxb8-SCF cells toward neutrophils was allowed by culture for 4 days in differentiation medium consisting of RPMI 1640 supplemented with 10% FCS, 1% penicillin/streptomycin, 20 ng/mL rmG-CSF (Peprotech), and 2% SCF-containing supernatant as described ( ). .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500 g , 30 minutes, room temperature). ..

Article Title: GPR97 triggers inflammatory processes in human neutrophils via a macromolecular complex upstream of PAR2 activation.
Article Snippet: .. In brief, blood samples were drawn into the collection tubes coated with sodium heparin and neutrophil isolation was performed using PolymorphprepTM density gradient centrifugation (Axis-Shield, Oslo, Norway) as described previously18. .. For the isolation ofmurine neutrophils, blood was collected by cardiac puncture and separated by Ficoll (GE Healthcare) gradient separation.

Article Title: Mitochondrial N-formyl methionine peptides contribute to exaggerated neutrophil activation in patients with COVID-19
Article Snippet: .. Whole blood from healthy individuals was used for neutrophil isolation by using Polymorphprep density gradient (Axis-Shield, Dundee, UK) as described previously [ ]. ..

Article Title: Streptococcus pyogenes Hijacks Host Glutathione for Growth and Innate Immune Evasion
Article Snippet: .. Briefly, human neutrophils were isolated from fresh heparinized whole blood using PolymorphPrep density gradient centrifugation (Axis-Shield) as per the manufacturer’s instructions. ..

Centrifugation:

Article Title: A20 and the noncanonical NF- κ B pathway are key regulators of neutrophil recruitment during fetal ontogeny
Article Snippet: Differentiation of Hoxb8-SCF cells toward neutrophils was allowed by culture for 4 days in differentiation medium consisting of RPMI 1640 supplemented with 10% FCS, 1% penicillin/streptomycin, 20 ng/mL rmG-CSF (Peprotech), and 2% SCF-containing supernatant as described ( ). .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500 g , 30 minutes, room temperature). ..

Article Title: A20 and the noncanonical NF-κB pathway are key regulators of neutrophil recruitment during fetal ontogeny.
Article Snippet: .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500g, 30 minutes, room temperature). ..

Purification:

Article Title: A Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4
Article Snippet: .. Blood samples were immediately subjected to PMNs purification by Polymorphprep gradient centrifugation (Axis-Shield, Norseland) according to the manufacturer's protocol. ..

Gradient Centrifugation:

Article Title: A Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4
Article Snippet: .. Blood samples were immediately subjected to PMNs purification by Polymorphprep gradient centrifugation (Axis-Shield, Norseland) according to the manufacturer's protocol. ..

Article Title: GPR97 triggers inflammatory processes in human neutrophils via a macromolecular complex upstream of PAR2 activation.
Article Snippet: .. In brief, blood samples were drawn into the collection tubes coated with sodium heparin and neutrophil isolation was performed using PolymorphprepTM density gradient centrifugation (Axis-Shield, Oslo, Norway) as described previously18. .. For the isolation ofmurine neutrophils, blood was collected by cardiac puncture and separated by Ficoll (GE Healthcare) gradient separation.

Article Title: Streptococcus pyogenes Hijacks Host Glutathione for Growth and Innate Immune Evasion
Article Snippet: .. Briefly, human neutrophils were isolated from fresh heparinized whole blood using PolymorphPrep density gradient centrifugation (Axis-Shield) as per the manufacturer’s instructions. ..



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