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4 6 diamino 2 phenylindole dapi  (Beyotime)


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    Structured Review

    Beyotime 4 6 diamino 2 phenylindole dapi

    4 6 Diamino 2 Phenylindole Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI/pmc13657243-22-0-3
    Average 99 stars, based on 32901 article reviews
    4 6 diamino 2 phenylindole dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system"

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

    Journal: iScience

    doi: 10.1016/j.isci.2026.117149


    Figure Legend Snippet:

    Techniques Used: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software

    Related Articles

    Incubation:

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
    Article Snippet: NIH‐3T3 cells, RAW264.7 cells, or 4T1/MCF7 cells treated with 40 ng mL −1 rCXCL10 were seeded onto the coverslips and cultured for 72 h. Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.2% Triton X‐100 (Solarbio, T8200) for 5 min, and blocked with 5% BSA (Solarbio, A8850) for 2 h. For tissue sections, slides underwent deparaffinization, rehydration, and blocking with 5% BSA for 2 h. Both coverslips and tissue sections were incubated overnight at 4°C with primary antibodies against α‐SMA (Affinity, AF1032), CD206 (Affinity, DF4149), or β‐Catenin (Proteintech, PRO‐CY‐50WY). .. After three washes, samples were incubated for 1 h at room temperature in the dark with Fluor488‐conjugated secondary antibody (Immunoway, RS32109), counterstained with DAPI (Beyotime, C1005) for 3 min, and mounted with antifade medium (Abbkine, BMU104). ..

    Staining:

    Article Title: Targeting REV7 confers cervical cancer cell radiosensitivity and ferroptosis by negatively regulating SFXN1
    Article Snippet: The cells were incubated with the antibody against γH2AX (1:1000, Abcam, Cambridge, UK) overnight at 4 °C and then incubated with Cy3-labeled goat anti-mouse IgG (1:1000, Beyotime) for 30 min at 37 °C in the dark. .. Nuclei were stained with DAPI (Beyotime) for 5 min. .. Finally, images were acquired using an FV1200 Olympus confocal microscope (Olympus, Tokyo, Japan) with γH2AX (Excitation wavelength: 550 nm, emission wavelength: 570 nm; exposure time: 1000 ms) and DAPI (Excitation wavelength: 358 nm, emission wavelength: 461 nm; exposure time: 1000 ms).

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark. .. Finally, the fluorescence images of the stained cells were obtained using a confocal laser scanning microscope (Leica TCS SP8, Leica Microsystems, Wetzlar, Germany) and analyzed with ImageJ software.

    Lysis:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: Mouse ELISA kits for quantifying IL‐6, IL‐1β, TNF‐α, and IFN‐β were supplied by Beijing Solarbio Science & Technology Co., Ltd. (China). .. DAPI, PMSF, and RIPA lysis buffer were acquired from Beyotime Biotechnology (China). .. Antibodies targeting phospho‐TBK1 (Ser172, #5483), phospho‐STING (Ser365, #72971), total TBK1 (#38066), and total STING (#13647) were purchased from Cell Signaling Technology (CST) via Beijing Chengzhi Kewei Biotechnology Co., Ltd. (China).

    Fluorescence:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Microscopy:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..



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    Beyotime dapi
    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole <t>(DAPI)</t> <t>staining</t> at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).
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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    Journal: iScience

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

    doi: 10.1016/j.isci.2026.117149

    Figure Lengend Snippet:

    Article Snippet: 4′,6-diamino-2-phenylindole (DAPI) , Beyotime Biotechnology, China , Cat#C1002.

    Techniques: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software

    Journal: iScience

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    doi: 10.1016/j.isci.2026.117673

    Figure Lengend Snippet:

    Article Snippet: DAPI Fluoromount-G , SouthernBiotech, Birmingham, Alabama, USA , Cat#0100–20.

    Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon

    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Journal: iScience

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

    doi: 10.1016/j.isci.2026.117739

    Figure Lengend Snippet: IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Article Snippet: Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark.

    Techniques: Migration, Control, Expressing, Analysis, EdU Assay, Fluorescence, Staining, Transwell Migration Assay, Transwell Assay