dapi (Beyotime)
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dapi
Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi/DAPI/pm42894734-151-4-6
Average 99 stars, based on 33033 article reviews
Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi/DAPI/pm42894734-151-4-6
Average 99 stars, based on 33033 article reviews
dapi - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Zyxin protects against hypertensive nephropathy by restraining FAK/Skp2-CDK8/YAP signaling in tubular epithelial cells. Article Snippet: 1 Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Nephrology, Huadong Hospital, Fudan University, Yan’an Road No. 221, Shanghai 200040, China Abstract Background Tubulointerstitial fibrosis and inflammation are hallmark pathological features of hypertensive nephropathy (HN), yet the underlying molecular mechanisms remain elusive.. Zyxin, a focal adhesion protein, has been implicated in hypertension-related pathologies, but its specific role in HN-induced tubulointerstitial injury remains undefined.. Methods An HN murine model was established via a multi-hit DOCA/Ang II + uninephrectomy + high salt approach. Article Title: SERINC3 promotes oral squamous cell carcinoma progression and mitochondrial metabolic reprogramming involving c-MET/JNK/PGC-1α axis. Article Snippet: .. Cells were incubated with the working solution (100 nM) at 37°C for 20 min and fixed with 4% paraformaldehyde for 20 min. Then cells were washed again with PBS and treated with a fluorescence quenching agent containing Article Title: Dihydroquercetin Activates AMPK/SIRT1/PGC-1α Signaling to Attenuate Arsenic Trioxide-Induced Apoptosis and Ferroptosis in AC16 Cells and HUVECs. Article Snippet: .. The cells were fixed in 4% paraformaldehyde for 10 min, permeabilized with 0.1% Triton X-100 for 10 min, and sealed with 0.1% goat serum for 1 h. Cells were incubated first with primary antibody γ-H2AX (1:200, A11097, ABclonal, Woburn, MA, USA) at 4 ◦C overnight and then with fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit IgG antibody (1:200, A0468, Beyotime, Shanghai, China) for https://doi.org/10.3390/ijms27198753 1 h. The cells were incubated with Staining:Article Title: Zyxin protects against hypertensive nephropathy by restraining FAK/Skp2-CDK8/YAP signaling in tubular epithelial cells. Article Snippet: 1 Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Nephrology, Huadong Hospital, Fudan University, Yan’an Road No. 221, Shanghai 200040, China Abstract Background Tubulointerstitial fibrosis and inflammation are hallmark pathological features of hypertensive nephropathy (HN), yet the underlying molecular mechanisms remain elusive.. Zyxin, a focal adhesion protein, has been implicated in hypertension-related pathologies, but its specific role in HN-induced tubulointerstitial injury remains undefined.. Methods An HN murine model was established via a multi-hit DOCA/Ang II + uninephrectomy + high salt approach. Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer. Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with Article Title: Platelet-derived microparticles induce CD8 + T cell senescence in chronic kidney disease via the transfer of microRNA-550a-5p. Article Snippet: .. Nuclei were stained with Glass:Article Title: HOXA3 Knockdown Impairs hESC-Derived Thymic Epithelial-like Cell Differentiation and CXCL12-Associated Early T-Lineage Support. Article Snippet: .. Nuclei were counterstained with Fluorescence:Article Title: SERINC3 promotes oral squamous cell carcinoma progression and mitochondrial metabolic reprogramming involving c-MET/JNK/PGC-1α axis. Article Snippet: .. Cells were incubated with the working solution (100 nM) at 37°C for 20 min and fixed with 4% paraformaldehyde for 20 min. Then cells were washed again with PBS and treated with a fluorescence quenching agent containing Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer. Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with Confocal:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer. Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with Microscopy:Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer. Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with Modification:Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes. Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs). Saline:Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes. Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs). |
