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cdna microarray experiment  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cdna microarray experiment
    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
    Cdna Microarray Experiment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+experiment/pmc04432801-150-19-18
    Average 90 stars, based on 1 article reviews
    cdna microarray experiment - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence"

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2015.00069

    The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
    Figure Legend Snippet: The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Techniques Used: Microarray, Western Blot, Expressing, Transfection, Electrophoresis

    Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.
    Figure Legend Snippet: Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Techniques Used: Microarray, Quantitative RT-PCR, Blocking Assay, Real-time Polymerase Chain Reaction

    Verification of  microarray  data by quantitative real-time PCR .
    Figure Legend Snippet: Verification of microarray data by quantitative real-time PCR .

    Techniques Used: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay

    Related Articles

    Microarray:

    Article Title: Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma
    Article Snippet: .. Recently, our group performed an Affymetrix cDNA microarray to compare differentially expressed genes between 10 pairs of ESCC tumors and their adjacent non-tumorous tissues (Data have been submitted to Gene Expression Omnibus under the accession number GSE33810). ..

    Article Title: OR7-001 – By chip pyrin binds the IRF2 promoter
    Article Snippet: .. RNA interference (RNAi) technique coupled with Affymetrix cDNA microarray analysis was employed to compare gene expression profiles between the human myeloid leukemia cell line, THP.1, expressing endogenous pyrin (scrambled control, SC) and cells in which the gene had been knocked down (si MEFV ). ..

    Article Title: Functional characterization and gene expression profiling of Drosophila melanogaster short dADA2b isoform-containing dSAGA complexes
    Article Snippet: .. We compared the mRNA profiles of wild type, dAda2b null mutants and the dAda2bS-EGFP transgene-carriers in dAda2b d842 background. mRNA samples were obtained from late L3 larvae synchronized to anterior spiracle eversion and hybridized to cDNA microarray (Affymetrix Drosophila Genome 2.0 Array) containing 18 000 target sequences. ..

    Article Title: Redefinition of Affymetrix probe sets by sequence overlap with cDNA microarray probes reduces cross-platform inconsistencies in cancer-associated gene expression measurements
    Article Snippet: .. The gene expression profiles of the NCI-60 cell line panel measured by cDNA microarray and by Affymetrix HuFL oligo chips constitutes a unique data source. ..

    Article Title: Application of a correlation correction factor in a microarray cross-platform reproducibility study
    Article Snippet: The C3B microarray platform comprises 10,000 genes represented by 3' fifty-mer oligonucleotides (MWG Biotech) that were spotted in duplicate. .. Finally, a cDNA microarray spotted with full and partial length PCR probes (Research Genetics/Invitrogen) was used in George Mason University's (GMU) Center for Biomedical Genomics and Informatics. ..

    Article Title: Integrating bioinformatic resources to predict transcription factors interacting with cis -sequences conserved in co-regulated genes
    Article Snippet: .. The annotation procedure of cDNA microarray data and Affymetrix ATH1 data has been described earlier [ , ]. ..

    Article Title: Biologicals and Fetal Cell Therapy for Wound and Scar Management
    Article Snippet: .. To identify differentially expressed genes in banked fetal dermal skin cells, we used cDNA microarray containing approximately 12,500 sequences (U95A human genome chip, Affymetrix UK, High Wycombe). ..

    Article Title: CBX7 Modulates the Expression of Genes Critical for Cancer Progression
    Article Snippet: .. Then, by using an Affymetrix cDNA microarray, we analysed the gene expression profile of thyroid carcinoma cells in which the CBX7 expression was restored, and identified several genes up- and down-regulated. ..

    Gene Expression:

    Article Title: Characterization of Tumor Suppressive Function of cornulin in Esophageal Squamous Cell Carcinoma
    Article Snippet: .. Recently, our group performed an Affymetrix cDNA microarray to compare differentially expressed genes between 10 pairs of ESCC tumors and their adjacent non-tumorous tissues (Data have been submitted to Gene Expression Omnibus under the accession number GSE33810). ..

    Article Title: OR7-001 – By chip pyrin binds the IRF2 promoter
    Article Snippet: .. RNA interference (RNAi) technique coupled with Affymetrix cDNA microarray analysis was employed to compare gene expression profiles between the human myeloid leukemia cell line, THP.1, expressing endogenous pyrin (scrambled control, SC) and cells in which the gene had been knocked down (si MEFV ). ..

    Article Title: Redefinition of Affymetrix probe sets by sequence overlap with cDNA microarray probes reduces cross-platform inconsistencies in cancer-associated gene expression measurements
    Article Snippet: .. The gene expression profiles of the NCI-60 cell line panel measured by cDNA microarray and by Affymetrix HuFL oligo chips constitutes a unique data source. ..

    Article Title: CBX7 Modulates the Expression of Genes Critical for Cancer Progression
    Article Snippet: .. Then, by using an Affymetrix cDNA microarray, we analysed the gene expression profile of thyroid carcinoma cells in which the CBX7 expression was restored, and identified several genes up- and down-regulated. ..

    Expressing:

    Article Title: OR7-001 – By chip pyrin binds the IRF2 promoter
    Article Snippet: .. RNA interference (RNAi) technique coupled with Affymetrix cDNA microarray analysis was employed to compare gene expression profiles between the human myeloid leukemia cell line, THP.1, expressing endogenous pyrin (scrambled control, SC) and cells in which the gene had been knocked down (si MEFV ). ..

    Article Title: CBX7 Modulates the Expression of Genes Critical for Cancer Progression
    Article Snippet: .. Then, by using an Affymetrix cDNA microarray, we analysed the gene expression profile of thyroid carcinoma cells in which the CBX7 expression was restored, and identified several genes up- and down-regulated. ..

    Control:

    Article Title: OR7-001 – By chip pyrin binds the IRF2 promoter
    Article Snippet: .. RNA interference (RNAi) technique coupled with Affymetrix cDNA microarray analysis was employed to compare gene expression profiles between the human myeloid leukemia cell line, THP.1, expressing endogenous pyrin (scrambled control, SC) and cells in which the gene had been knocked down (si MEFV ). ..

    Polymerase Chain Reaction:

    Article Title: Application of a correlation correction factor in a microarray cross-platform reproducibility study
    Article Snippet: The C3B microarray platform comprises 10,000 genes represented by 3' fifty-mer oligonucleotides (MWG Biotech) that were spotted in duplicate. .. Finally, a cDNA microarray spotted with full and partial length PCR probes (Research Genetics/Invitrogen) was used in George Mason University's (GMU) Center for Biomedical Genomics and Informatics. ..



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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    Image Search Results


    The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Western Blot, Expressing, Transfection, Electrophoresis

    Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Quantitative RT-PCR, Blocking Assay, Real-time Polymerase Chain Reaction

    Verification of  microarray  data by quantitative real-time PCR .

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Verification of microarray data by quantitative real-time PCR .

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay