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Biotechnology Information cdna microarray experiments
Cdna Microarray Experiments, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+experiment/cdna+microarray/pmc01287979-66-20-12
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cdna microarray experiments - by Bioz Stars, 2026-09
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Related Articles

Microarray:

Article Title: Inhibition of Intracellular Clusterin Attenuates Cell Death in Nephropathic Cystinosis
Article Snippet: .. All of the probes on the cDNA microarray platform were reannotated to the most recent National Center for Biotechnology Information gene identifiers ( http://ailun.stanford.edu ). ..

Article Title: Evolutionary regulation of the blind subterranean mole rat, Spalax , revealed by genome-wide gene expression
Article Snippet: When scaled to a target intensity of 150 (Affymetrix mas 5.0 array analysis software), scaling factors for all arrays were within acceptable limits, as were background, Q values, and mean intensities. .. Details of quality control measures are available from the National Center for Biotechnology Information or the Chaim Sheba Medical Center. cDNA Microarray Experiments. ..

Article Title: Method of diagnosing esophageal cancer
Article Snippet: .. Serum was obtained at the time of diagnosis and stored at −80° C. Clinicopathological records were fully documented. cDNA Microarray: A genome-wide cDNA microarray was fabricated with 32,256 cDNAs selected from the UniGene database (build #131) of the National Center for Biotechnology Information (NCBI). ..

Article Title: Upregulation of peroxisome proliferator-activated receptor-α and the lipid metabolism pathway promotes carcinogenesis of ampullary cancer
Article Snippet: .. The study began with a complementary (c)DNA microarray of patient samples validated by the National Center for Biotechnology Information (NCBI) dataset. ..

Article Title: Quantitative gene expression analysis by cDNA microarray during liver regeneration after partial hepatectomy in rats.
Article Snippet: Hepatocytes have a quiescent and highly differentiated phenotype.. They rarely divide in adult humans or animals while in the G0-phase of the cell cycle; however, their capacity to replicate is not lost and is readily activated after liver resection or injury induced by chemicals or drugs.. Therefore, hepatocytes constitute a conditional renewal cell system that may proliferate in vivo under well-defined conditions.1–3 It seems that the liver “knows” when to start and when to stop growing, and thereby accurately regulates its mass.4,5 Partial hepatectomy triggers hepatocyte proliferation, whereas excessive liver mass is regulated by apoptosis.

Article Title: EpCAM and alpha-fetoprotein expression defines novel prognostic subtypes of hepatocellular carcinoma.
Article Snippet: .. The cDNA microarray (9) and the oligonucleotide microarray data sets are publicly available at the National Center for Biotechnology Information Gene Expression Omnibus database (GEO accession nos. ..

Article Title: Method of diagnosing esophageal cancer
Article Snippet: .. Serum was obtained at the time of diagnosis and stored at −80° C. Clinicopathological records were fully documented. cDNA Microarray: A genome-wide cDNA microarray was fabricated with 32,256 cDNAs selected from the UniGene database (build #131) of the National Center for Biotechnology Information (NCBI). ..

Control:

Article Title: Evolutionary regulation of the blind subterranean mole rat, Spalax , revealed by genome-wide gene expression
Article Snippet: When scaled to a target intensity of 150 (Affymetrix mas 5.0 array analysis software), scaling factors for all arrays were within acceptable limits, as were background, Q values, and mean intensities. .. Details of quality control measures are available from the National Center for Biotechnology Information or the Chaim Sheba Medical Center. cDNA Microarray Experiments. ..

Biomarker Discovery:

Article Title: Method of diagnosing esophageal cancer
Article Snippet: .. Serum was obtained at the time of diagnosis and stored at −80° C. Clinicopathological records were fully documented. cDNA Microarray: A genome-wide cDNA microarray was fabricated with 32,256 cDNAs selected from the UniGene database (build #131) of the National Center for Biotechnology Information (NCBI). ..

Article Title: Method of diagnosing esophageal cancer
Article Snippet: .. Serum was obtained at the time of diagnosis and stored at −80° C. Clinicopathological records were fully documented. cDNA Microarray: A genome-wide cDNA microarray was fabricated with 32,256 cDNAs selected from the UniGene database (build #131) of the National Center for Biotechnology Information (NCBI). ..

Genome Wide:

Article Title: Method of diagnosing esophageal cancer
Article Snippet: .. Serum was obtained at the time of diagnosis and stored at −80° C. Clinicopathological records were fully documented. cDNA Microarray: A genome-wide cDNA microarray was fabricated with 32,256 cDNAs selected from the UniGene database (build #131) of the National Center for Biotechnology Information (NCBI). ..

Article Title: Method of diagnosing esophageal cancer
Article Snippet: .. Serum was obtained at the time of diagnosis and stored at −80° C. Clinicopathological records were fully documented. cDNA Microarray: A genome-wide cDNA microarray was fabricated with 32,256 cDNAs selected from the UniGene database (build #131) of the National Center for Biotechnology Information (NCBI). ..

Hybridization:

Article Title: Quantitative gene expression analysis by cDNA microarray during liver regeneration after partial hepatectomy in rats.
Article Snippet: Hepatocytes have a quiescent and highly differentiated phenotype.. They rarely divide in adult humans or animals while in the G0-phase of the cell cycle; however, their capacity to replicate is not lost and is readily activated after liver resection or injury induced by chemicals or drugs.. Therefore, hepatocytes constitute a conditional renewal cell system that may proliferate in vivo under well-defined conditions.1–3 It seems that the liver “knows” when to start and when to stop growing, and thereby accurately regulates its mass.4,5 Partial hepatectomy triggers hepatocyte proliferation, whereas excessive liver mass is regulated by apoptosis.

Gene Expression:

Article Title: Quantitative gene expression analysis by cDNA microarray during liver regeneration after partial hepatectomy in rats.
Article Snippet: Hepatocytes have a quiescent and highly differentiated phenotype.. They rarely divide in adult humans or animals while in the G0-phase of the cell cycle; however, their capacity to replicate is not lost and is readily activated after liver resection or injury induced by chemicals or drugs.. Therefore, hepatocytes constitute a conditional renewal cell system that may proliferate in vivo under well-defined conditions.1–3 It seems that the liver “knows” when to start and when to stop growing, and thereby accurately regulates its mass.4,5 Partial hepatectomy triggers hepatocyte proliferation, whereas excessive liver mass is regulated by apoptosis.

Article Title: EpCAM and alpha-fetoprotein expression defines novel prognostic subtypes of hepatocellular carcinoma.
Article Snippet: .. The cDNA microarray (9) and the oligonucleotide microarray data sets are publicly available at the National Center for Biotechnology Information Gene Expression Omnibus database (GEO accession nos. ..



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The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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Biotechnology Information cdna microarray experiments
The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
Cdna Microarray Experiments, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+experiment/cdna+microarray/pmc01287979-66-20-12
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The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

Journal: Frontiers in Endocrinology

Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

doi: 10.3389/fendo.2015.00069

Figure Lengend Snippet: The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

Techniques: Microarray, Western Blot, Expressing, Transfection, Electrophoresis

Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

Journal: Frontiers in Endocrinology

Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

doi: 10.3389/fendo.2015.00069

Figure Lengend Snippet: Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

Techniques: Microarray, Quantitative RT-PCR, Blocking Assay, Real-time Polymerase Chain Reaction

Verification of  microarray  data by quantitative real-time PCR .

Journal: Frontiers in Endocrinology

Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

doi: 10.3389/fendo.2015.00069

Figure Lengend Snippet: Verification of microarray data by quantitative real-time PCR .

Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay