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basic nucleofector kit for primary mammalian endothelial cells  (Lonza)


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    Structured Review

    Lonza basic nucleofector kit for primary mammalian endothelial cells
    KEY RESOURCES TABLE
    Basic Nucleofector Kit For Primary Mammalian Endothelial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+kit/basic+nucleofector+kit+for+primary+mammalian+endothelial+cells/pmc11562893-84-0-9
    Average 90 stars, based on 1 article reviews
    basic nucleofector kit for primary mammalian endothelial cells - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy"

    Article Title: Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114848

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Plasmid Preparation, Recombinant, Marker, Control, Saline, Lysis, Protease Inhibitor, Stripping Membranes, Purification, Bicinchoninic Acid Protein Assay, RNAscope, Multiplex Assay, Negative Control, Software, Staining, Microscopy

    Related Articles

    Binding Assay:

    Article Title: KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogram the endothelial enhancer landscape under laminar shear stress
    Article Snippet: PAEC were transduced with a lentiviral vector encoding a doxycylin-inducible mutant of a catalytically dead Cas9 fused to the bipartite repressor domain MeCP2-KRAB, as described above. .. Cells were treated with 500 ng/μl of doxycycline 48 h prior to nucleofection of synthetic guide RNAs (sgRNA) (Synthego) using the Basic Nucleofector Kit for Primary Mammalian Endothelial Cells (Lonza). sgRNA were designed to target the KLF binding sites in the candidate enhancers using the GPP sgRNA Design tool from the Broad Institute ( https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design ). ..

    Article Title: KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogram the endothelial enhancer landscape under laminar shear stress.
    Article Snippet: PAEC were transduced with a lentiviral vector encoding a doxycylininducible mutant of a catalytically dead Cas9 fused to the bipartite repressor domain MeCP2-KRAB, as described above. .. Cells were treated with 500 ng/μl of doxycycline 48 h prior to nucleofection of synthetic guide RNAs (sgRNA) (Synthego) using the Basic Nucleofector Kit for Primary Mammalian Endothelial Cells (Lonza). sgRNAwere designed to target the KLF binding sites in the candidate enhancers using the GPP sgRNA Design tool from the Broad Institute (https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrnadesign). ..

    Electroporation:

    Article Title: Decreasing expression of the G 1 -phase inhibitors, p21Cip1 and p16INK4a, promotes division of corneal endothelial cells from older donors
    Article Snippet: .. An Amaxa Nucleofector II device (Lonza, Basel, Switzerland) and a Basic Nucleofector Kit for Primary Mammalian Endothelial Cells using the T-23 program were used for electroporation. ..

    Transfection:

    Article Title: PLAC8-Mediated Activation of NOX4 Signalling Restores Angiogenic Function of Endothelial Colony-Forming Cells in Experimental Hypoxia
    Article Snippet: .. For NOX4 OE, up to 5 × 10 5 cells were resuspended in 100 μL transfection solution and electroporated using the Amaxa ® system and Basic Nucleofector Kit for Primary Mammalian Endothelial Cells (Lonza, Basel, Switzerland) together with 1 μg of either pcDNA4/TO/myc-His empty vector (EV) or pcDNA4/TO/NOX4-myc-His A expression plasmid containing a full-length myc-tagged copy of NOX4. .. PLAC8 KD was achieved by transfection of 1 × 10 6 cells with 20 nmol/L of SMARTpool siRNA (DharmaconTM, Lafayette, CO, USA) against PLAC8 or non-targeting scrambled (SCR) control using DharmaFECT1 ® transfection reagent (Horizon Discovery, Waterbeach, UK) for 24 h. Transfected CB-ECFCs were maintained in EGM2 complete medium for at least 24 h until further experiments.

    Expressing:

    Article Title: PLAC8-Mediated Activation of NOX4 Signalling Restores Angiogenic Function of Endothelial Colony-Forming Cells in Experimental Hypoxia
    Article Snippet: .. For NOX4 OE, up to 5 × 10 5 cells were resuspended in 100 μL transfection solution and electroporated using the Amaxa ® system and Basic Nucleofector Kit for Primary Mammalian Endothelial Cells (Lonza, Basel, Switzerland) together with 1 μg of either pcDNA4/TO/myc-His empty vector (EV) or pcDNA4/TO/NOX4-myc-His A expression plasmid containing a full-length myc-tagged copy of NOX4. .. PLAC8 KD was achieved by transfection of 1 × 10 6 cells with 20 nmol/L of SMARTpool siRNA (DharmaconTM, Lafayette, CO, USA) against PLAC8 or non-targeting scrambled (SCR) control using DharmaFECT1 ® transfection reagent (Horizon Discovery, Waterbeach, UK) for 24 h. Transfected CB-ECFCs were maintained in EGM2 complete medium for at least 24 h until further experiments.

    Plasmid Preparation:

    Article Title: PLAC8-Mediated Activation of NOX4 Signalling Restores Angiogenic Function of Endothelial Colony-Forming Cells in Experimental Hypoxia
    Article Snippet: .. For NOX4 OE, up to 5 × 10 5 cells were resuspended in 100 μL transfection solution and electroporated using the Amaxa ® system and Basic Nucleofector Kit for Primary Mammalian Endothelial Cells (Lonza, Basel, Switzerland) together with 1 μg of either pcDNA4/TO/myc-His empty vector (EV) or pcDNA4/TO/NOX4-myc-His A expression plasmid containing a full-length myc-tagged copy of NOX4. .. PLAC8 KD was achieved by transfection of 1 × 10 6 cells with 20 nmol/L of SMARTpool siRNA (DharmaconTM, Lafayette, CO, USA) against PLAC8 or non-targeting scrambled (SCR) control using DharmaFECT1 ® transfection reagent (Horizon Discovery, Waterbeach, UK) for 24 h. Transfected CB-ECFCs were maintained in EGM2 complete medium for at least 24 h until further experiments.



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