basic nucleofector kit for primary smooth muscle cells (Lonza)

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Lonza basic nucleofector kit for primary smooth muscle cells
Basic Nucleofector Kit For Primary Smooth Muscle Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/basic+nucleofector+kit/basic+nucleofector+kit+primary+mammalian+smooth+muscle+cells/pmc03299759-62-19-22
Average 90 stars, based on 1 article reviews

basic nucleofector kit for primary smooth muscle cells - by Bioz Stars, 2026-11

90/100 stars

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Transfection:

Article Title: Regulation of Smooth Muscle Cell Proliferation by Mitochondrial Ca2+ in Type 2 Diabetes
Article Snippet: .. VSMCs were transfected in a Nucleofector I device (Lonza, Basel, Switzerland) using the Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells (VPI-1004, Lonza) and following the manufacturer’s protocol. .. 50,000 cells were transfected in the presence of 1 μg of plasmid DNA, plated onto 35-mm glass bottom microwell dishes (MatTek Corporation), and grown for 48 h before experiments were performed.

Article Title: The mitochondrial regulation of smooth muscle cell proliferation in type 2 diabetes
Article Snippet: .. VSCMs were transfected in a Nucleofector I device (Lonza) using the Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells (VPI-1004, Lonza) and following the manufacturer’s protocol. .. Six hundred thousand cells were transfected in the presence of 5-μg of plasmid DNA, plated onto 35-mm glass bottom microwell dishes (MatTek Corporation), and grown for 48 hr before experiments were performed.

Article Title: Loss of MCU prevents mitochondrial fusion in G 1 -S phase and blocks cell cycle progression and proliferation.
Article Snippet: .. VSCMs were transfected in a Nucleofector I device (Lonza) with the Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells (#VPI1004, Lonza), following the manufacturer’s protocol. .. Six hun dred thousand cells were transfected in the presence of 5g plasmid DNA, plated onto 35mm glass bottom microwell dishes (MatTek Cor poration), and grown for 72 hours before experiments were performed.

Article Title: Increased ectodysplasin-A2-receptor EDA2R is a ubiquitous hallmark of aging and mediates parainflammatory responses.
Article Snippet: .. Nature Communications | (2025) 16:1898 5 electroporation, skeletal muscle myoblasts isolated from a human male donor were transfected using the AmaxaTM Nucleofector Basic Kit for Primary Mammalian Smooth Muscle Cells (Lonza, VPI-1004). ..

Plasmid Preparation:

Article Title: MIRO1 Is Required for Dynamic Increases in Mitochondria-ER Contact Sites and Mitochondrial ATP During the Cell Cycle.
Article Snippet: VSMCs or skin fibroblasts were transduced with the adenovirus construct at a multiplicity of infection (MOI) of 50 for 48 h. The SPLICS Mt-ER short P2A (# 164108) plasmid was purchased from Addgene. .. VSMCs were electroporated with the SPLICSS plasmid in a NucleofectorTM I device (Lonza) with the Basic NucleofectorTM Kit for Primary Mammalian Smooth Muscle Cells (Lonza # VPI-1004), following the manufacturer’s protocol. .. A total of 50,000 cells were electroporated in the presence of 5 μg plasmid DNA, plated onto 35 mm glass-bottom microwell dishes (MatTek Corporation, Ashland, MA, USA, Cat# P35G-1.5-20-C) and cultured for 48 h before experiments were performed.

Electroporation:

Isolation:

other:

Article Title: Mural Cell SDF1 Signaling Is Associated with the Pathogenesis of Pulmonary Arterial Hypertension
Article Snippet: All images were captured using a Zeiss confocal 880 Airyscan 2.

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