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nucleofection reagents for primary mammalian glial cells  (Lonza)


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    Structured Review

    Lonza nucleofection reagents for primary mammalian glial cells
    Nucleofection Reagents For Primary Mammalian Glial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/basic+nucleofector+kit/basic+nucleofector+kit+for+primary+mammalian+glial+cells/pm39936951-80-15-28
    Average 90 stars, based on 1 article reviews
    nucleofection reagents for primary mammalian glial cells - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Negative Control:

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice
    Article Snippet: Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [ , ]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2 -specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-Nucleofector TM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice.
    Article Snippet: Screening of Atp1a2-specific ASOs in primary astrocytes Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [23, 24]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2-specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Allele-specific Oligonucleotide:

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice
    Article Snippet: Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [ , ]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2 -specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-Nucleofector TM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Article Title: Targeted ASO-mediated Atp1a2 knockdown in astrocytes reduces SOD1 aggregation and accelerates disease onset in mutant SOD1 mice.
    Article Snippet: Screening of Atp1a2-specific ASOs in primary astrocytes Primary murine astrocytes used in this study were generated from the cortices of post-natal day 2 (P2) pups (n = 10–12) from either CD1 wild-type strain (code: 022, #24101187 from Charles River Laboratories) or SOD1*G93A mice as previously described [23, 24]. .. Primary murine astrocytes were nucleofected with a non-targeting negative control A (ctrl ASO) or Atp1a2-specific ASOs at several concentrations using the AmaxaTM Basic NucleofectorTM Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) or P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza, V4XP-3032) following the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: Astrocyte-Secreted Lcn2 Modulates Dendritic Spine Morphology
    Article Snippet: .. For the experiments, the astrocytic cells were electroporated with 5 μg of pZac_gfa ABC1D _Lcn2_FLAG_SEP plasmid using nucleofection reagents for primary mammalian glial cells, according to the manufacturer’s protocol (Amaxa, Lonza, Basel, Switzerland). ..

    Article Title: Astrocyte-Secreted Lcn2 Modulates Dendritic Spine Morphology.
    Article Snippet: .. For the experiments, the astrocytic cells were electroporated with 5 μg of pZac_gfaABC1D_Lcn2_FLAG_SEP plasmid using nucleofection reagents for primary mammalian glial cells, according to the manufacturer’s protocol (Amaxa, Lonza, Basel, Switzerland). ..

    Construct:

    Article Title: Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
    Article Snippet: .. For ACM production, during the astrocyte passage step (DIV 7), the astrocytes were nucleofected with constructs carrying either a scramble sequence (shCtrl, 5’-GCTCACCACGTGCAATACTAT-3’) or shRNA (shAtp13a4, 5’-GCCCATGAACTTCAAGCTCTA −3’), using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, Cat# VPI-1006) according to the manufacturer’s protocol. ..

    Sequencing:

    Article Title: Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
    Article Snippet: .. For ACM production, during the astrocyte passage step (DIV 7), the astrocytes were nucleofected with constructs carrying either a scramble sequence (shCtrl, 5’-GCTCACCACGTGCAATACTAT-3’) or shRNA (shAtp13a4, 5’-GCCCATGAACTTCAAGCTCTA −3’), using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, Cat# VPI-1006) according to the manufacturer’s protocol. ..

    shRNA:

    Article Title: Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
    Article Snippet: .. For ACM production, during the astrocyte passage step (DIV 7), the astrocytes were nucleofected with constructs carrying either a scramble sequence (shCtrl, 5’-GCTCACCACGTGCAATACTAT-3’) or shRNA (shAtp13a4, 5’-GCCCATGAACTTCAAGCTCTA −3’), using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, Cat# VPI-1006) according to the manufacturer’s protocol. ..

    Isolation:

    Article Title: The AMPK activator metformin improves recovery from demyelination by shifting oligodendrocyte bioenergetics and accelerating OPC differentiation
    Article Snippet: .. Nucleofection of isolated OPCs was performed with siRNA mixtures to target both AMPKα 1 (Dharmacon, Cat. No. L-091373-02-0005) and AMPKα 2 (Dharmacon, Cat. No. L-100623-02-0005), using the basic nucleofector kit for primary mammalian glial cells (Lonza Biosciences). .. Control cells were transfected with a pool of non-targeting siRNAs (Dharmacon).



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