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All (22)
Addgene
Genechem
Thermo Fisher
OriGene
Promega
NovoPro Biosciences
Vigene
Becton Dickinson
14 More...
 

About 94 products / 157 snippets / 31 images

93 / 100
Bioz vStars
pLVX-puro-Cas9 Vector
Takara Bio  
Catalog#: 632631
94 / 100
Bioz Stars
pLVX-puro-cOVA (Plasmid #135073)
Addgene  
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Catalog#: 135073
Techniques
Cell Culture, Plasmid Preparation, Expressing, Transfection, Generated (see more)
Article Snippets
"MC38-OVA cells were generated by transducing MC38 cells with pLVX-puro-cOVA (Addgene plasmid #135073) followed by selection in puromycin (4 μg/mL)."  (see more)
94 / 100
Bioz Stars
pLVXPuro-mGFP-LIC1 (Plasmid #66600)
Addgene  
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Catalog#: 66600
Techniques
Plasmid Preparation, Expressing, Construct, Transfection, Cell Culture (see more)
Article Snippets
".. dynactin complex components were also obtained through Addgene: DYNC1LI1 and DYNC1LI2 respectively pLVXPuro-mGFP-LIC1/-LIC2 (cat #66600 and #66601)."  (see more)
27+  more images
A – B: Still images from confocal live cell imaging of RPE-1 stably expressing GFP-COL (green; GFP-COL-RPE) co-transfected with the inner-layer COPII marker <t>mSc-Sec23A</t> (magenta) derived from Video 9. Acquisition at 1 frame every 2.79 seconds. Time points indicate time in presence of asc/biotin (500 μg.ml-1 and 400 μM, respectively) and 20 hours post-transfection. Scale bar = 10 μm (0.25 μm for enlargements) and n = 9. A: Large panels show the entire cell with enlargements displayed in the top right corner. B: Shows the zoomed in area of interest from A (also indicated by the square) with the separate channels in greyscale, followed by the overlay image. Arrow heads indicate a GFP-COL structure that colocalises with a mSc-Sec23A structure and at about 1 min 38 sec before the GFP-COL structure becomes part of the Golgi network. Scale bar = 0.25 μm. C: Example image of a GFP-COL-RPE cells co-transfected with ManII-mSc and the GTP-locked form of Sar1 (Sar1-H79G), which results in a COPII block. Panels show the separate channels GFP-COL (green) and MannII-mSc (red), as well as antibody labelling with a cis-Golgi marker giantin, in greyscale followed by the overlay image including nuclear labelling for DAPI (imaged as a separate channel in pseudo colour magenta). The time point indicates the incubation in presence of asc/biotin prior to fixation with PFA and 7.5 hours after transfection. Scale bar = 10 μm. Cells expressing Sar1H79G show a scattered a disrupted Golgi apparatus, marked by ER-labelling, compared to visible Golgi stacks in cells not affected by the COPII block (i). No transport of GFP-COL can be observed in COPII blocked cells (ii and iii).
LIVE-CELL
STAB-TRANS
EXP
TNSF
MARK
(a–c) ARID1A and a loading control GAPDH protein expression in ARID1A wildtype RMG1 cells with or without ARID1A knockdown (a). Cells were transduced with <t>lentivirus</t> encoding shRNA to each of the 11 individual HDACs (HDAC1-11) and subjected to colony formation assay. Scatterplot of the integrated density normalized to control. The x-axis indicates changes in cell growth induced by individual shHDACs in control ARID1A wildtype treated cells, while the y-axis indicates changes in cell growth induced by the same shHDACs in shARID1A-expressing cells. Individual HDACs are indicated with their respective numbers (b). n=4 independent experiments. Colony formation by the indicated cells (c). (d–f) ARID1A protein expression in parental and ARID1A CRIPSR OVCA429 cells (d). Colony formation assay using the indicated OVCA429 cells with or without HDAC6 knockdown (e), which was quantified (f). n=3 independent experiments. (g–h) A panel of cell lines with known ARID1A mutational status with or without HDAC6 knockdown were grown in 3D using Matrigel. Shown are acini formed by the indicated cells (g). Scale bar = 75 AU in NIH Image J software. The diameters of acini (n=50; representative of three biological repeats) were quantified (h). n.s. indicates not significant. Error bars represent mean with S.E.M. P -value calculated via two-tailed t -test. Statistical source data are provided in . Unprocessed original scans of all blots with size marker are shown in .
CON
EXP
KD
TRANSDUC
shRNA
ANLN promotes PCa cell proliferation in vitro. A-D. qPCR and western blot analysis showing the knockdown and <t>overexpression</t> efficiency of ANLN. ****P < 0.0001. E-H. Analysis of cell viability in ANLN knockdown and overexpression prostate cancer cells using CCK-8 kit. **P < 0.01, ***P < 0.001, ****P < 0.0001. I-L. Colony formation assay was performed to determine the proliferation of PC-3 and DU145 cells harboring the different vectors indicated. ****P < 0.0001. M-P. Result of EdU assay showing the cell proliferation in ANLN knockdown and overexpression prostate cancer cells. **P < 0.01, ****P < 0.0001. Scale bar = 100 μm.
INVITRO
WB
KD
OVER-EXP
CCK-8
Primer pairs were designed to generate insert(s) from DNA template(s) via PCR.
PLASMID-P
SEQ
×
94 / 100
Bioz Stars
pLVX-puro-cOVA-IRES-BFP (Plasmid #135074)
Addgene  
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Catalog#: 135074
Techniques
Plasmid Preparation, Isolation, Cell Culture, Expressing, Staining, Lysis (see more)
Article Snippets
"To generate a stable LL/2 cell line expressing OVA (LL/2-OVA), a lentiviral expression vector pLVX-puro-cOVA-IRES-BFP (Addgene #135074, Watertown, MA, USA) containing the cDNA .."  (see more)
94 / 100
Bioz Stars
pLVX-Puro-mRuby-Sec23A (Plasmid #36158)
Addgene  
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Catalog#: 36158
Techniques
Polymerase Chain Reaction, Generated, Plasmid Preparation, Sequencing (see more)
Article Snippets
".. first replaced with mScarlet-i and in a second step Sec23A from pLVX-Puro-mRuby-Sec23A (Addgene #36158; ( )) was amplified using PCR and inserted after Str-KDEL-IRES-mScarlet-i. .."  (see more)
LIVE-CELL
STAB-TRANS
EXP
TNSF
MARK
94 / 100
Bioz Stars
pLVX-Puro-hGSDME (Plasmid #154876)
Addgene  
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Catalog#: 154876
Techniques
Plasmid Preparation, Sequencing, Electrophoresis, Transfection (see more)
Article Snippets
".. (Addgene plasmid #111,559; http://n2t.net/addgene:111559 ; RRID:Addgene_111559). pLVX-Puro-hGSDME was a gift from Judy Lieberman (Addgene plasmid #154,876; http://n2t.net/addgene:154876 ; RRID:Addgene_154876). pGALL-(LEU2)-hGSDMD was made by amplifying the coding region of human gsdmd with primers #2117 .."  (see more)
PLASMID-P
SEQ
90 / 100
Bioz Stars
PLVX-Puro
Genechem  
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Techniques
 
Article Snippets
".. stable osteosarcoma cell lines overexpressing FHIT , lentivirus containing pLVX-FHIT-Puro and pLVX-Puro (vector) were purchased from Genechem (GeneChem, Shanghai, China) and were used to infect 143B and MG63 .."  (see more)
80 / 100
Bioz Stars
PLVX-puro
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
"For stable expression, PRPF19 WT was inserted into pLVX-puro (BD Biosciences Clontech, catalog no. 632164) as XhoI/XbaI fragments."  (see more)
91 / 100
Bioz Stars
PLVX-Puro
Addgene  
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Techniques
 
Article Snippets
".. were recloned and inserted into a third-generation lentiviral expression vector, pLVX-Puro (Addgene, 141395) under the regulation of a human EF-1α promoter."  (see more)
91 / 100
Bioz Stars
PLVX-puro
Addgene  
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Techniques
 
Article Snippets
".. GGCT/MRPL9, the GGCT/MRPL9DE CDS sequence was amplified from human cDNA and ligated into the pLVX-puro (Addgene, Watertown, MA, USA) vector."  (see more)
91 / 100
Bioz Stars
PLVX-Puro
Thermo Fisher Scientific / Invitrogen  
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Techniques
 
Article Snippets
"113 114 Cell transfection 115 116 Ectopic endometrial stromal cells (3×105 cell/well) were cultured in 6-well plates for 24 h and 117 then transfected with three siRNAs (siRNA#1, position 1583-1601, 5’-CCACCTGATGAAGTTCATT-3’; 118 siRNA#2, position 1895-1913, 5’-GCTTCTCTCACCAAGTAAT-3’; .."  (see more)
80 / 100
Bioz Stars
PLVX‐puro
Clonexpress inc  
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Techniques
 
Article Snippets
".. 48 h. For HA‐tagged TERT overexpressed cell line construction, HA‐tagged TERT was cloned into pLVX‐puro by ClonExpress II One Step Cloning Kit (Vazyme, C112‐01)."  (see more)
80 / 100
Bioz Stars
PLVX- Puro
GenScript  
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Techniques
 
Article Snippets
"Gria2 was amplified by PCR from cDNA and cloned into pLVX- Puro (GenScript, Cat# Z03528)."  (see more)
93 / 100
Bioz vStars
pLVX-puro-Cas9 Vector
Takara Bio  
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Catalog#: 632631