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All (18)
Cell Signaling
Santa Cruz
Proteintech
QIAGEN
ABclonal Biotech
OriGene
MilliporeSigma
Thermo Fisher
10 More...
 

About 44 products / 95 snippets / 39 images

91 / 100
Bioz Stars
NFκB1
Santa Cruz  
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Techniques
 
Article Snippets
"The membranes were incubated with NFκB1 (1:1000; Santa Cruz, CA) or anti-actin (1:5000; Sigma-Aldrich, USA) primary antibody at 4°C overnight."  (see more)
OVER-EXP
METHY
TNSF
CON
FUNC-A
91 / 100
Bioz Stars
NFκB1
Cell Signaling Technology  
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Techniques
 
Article Snippets
"® #3031), NFκB2 (p100 and p52) (Millipore © #05-361), NFκB1 (Cell Signaling Tech."  (see more)
35+  more images
Overexpression of miR-9 in CLL cells. I83-E95 cells, completely methylated for miR-9-3 , were transfected with miR-9 mimic or scrambled control oligo. Cells were harvested for functional assays 48 hours after transfection. A , Stem-loop RT-qPCR analysis of miR-9 expression at 48 hours after transfection. ΔCt, Ct miR-9 -Ct RNU48. RNU48 was chosen as reference using the 2 -ΔΔCT method. B , Cell proliferation of CLL cells in response to overexpression of miR-9 was measured by MTT assay. Column, mean percentage of cell proliferation from three experiments conducted in triplicate; C , Cellular death was measured by Trypan blue exclusion assay. Column, mean percentage of cell death from three experiments conducted in triplicate; D , Cell apoptosis of CLL cells after miR-9 transfection was assessed by flow cytometry using FITC Annexin V and PI staining (left panel: representative result; right panel: values represented mean percentage of cell apoptosis from three experiments performed in triplicate. E , Western blot analysis of <t>NFκB1</t> (P105/P50) after miR-9 transfection. Total protein was extracted 48 hours after transfection and membranes were probed with antibodies to P105, P50 and Anti-actin. Anti-actin protein was regarded as the endogenous normalizer and the relative NFκB1 (P105/P50) protein level was shown. Error bars represent standard deviation.
OVER-EXP
METHY
TNSF
CON
FUNC-A
PE6 induces the activation of the canonical NFκB signaling pathway. (A) Western blots showing the expression level of <t>NFκB1</t> (P50) subunit of NFκB in PE6 treated RAW264.7 macrophage cells. RAW264.7 cells were treated with PE6 (1 and 2 µg/ml) for 24 h. (B) Densitometric quantitation of the P50 subunit of canonical NFκB. P50 protein levels were expressed relative to GAPDH [%]. (C) Immunofluorescence microscopic pictures showing nuclear translocation of P50 subunit. (D) Western blots showing the protein levels of the phosphorylated pP65 (RelA) subunit of NFκB in PE6 treated RAW264.7 macrophage cells. (E) Densitometric quantitation of pP65 subunit. The pP65 protein levels were expressed relative to GAPDH [%]. (F) Immunofluorescence microscopic pictures showing nuclear localization of phosphorylated P65. DAPI was used to mark the nucleus. Untreated and HI PE6 treated cells were used as negative controls. LPS (500 ng and 1 µg/ml) treatment was used as a positive control. GAPDH was used as a loading control. A488 linked secondary antibody was used for signal detection. Scale bars indicate 10 µm. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison post-test. Data are representative of three independent experiments. *P < 0.05 and **P < 0.01 vs . controls.
ACTIV
WB
EXP
QUANT-A
IF
NFκB2 is required for FAS transactivation. A) Gel shift assay was performed to observe the retardation of GST, GST‐p50 or GST‐p52 to mobility of DNA probe covering 3′‐2a regions of FAS promoter. Probe and protein concentration were 20 n m . B) Gel shift assay was performed with probe 3′‐2a, GST‐p52 protein and increasing doses of GST‐PIR protein. The final concentration of probe was 20 n m . C) Gel shift assay was performed with probe 3′‐2a and indicated proteins. The final concentration of probe was 20 n m . D) Flag‐tagged PIR and HA‐tagged RELB were transiently expressed in HEK293T cells. Co‐IP assay were performed with anti‐Flag agarose (Flag IP), followed by detection of Flag‐PIR and HA‐RELB in the immuno‐precipitates. E) Co‐IP assays were performed with endogenous proteins to determine the association of PIR with RELB in HCT116 cells. F) Flag‐PIR, Flag‐RELB and HA‐p52 were transiently expressed in HEK293T cells. Co‐IP were performed to determine whether PIR disrupts the interaction between RELB and p52. G) Flag‐RELB and Flag‐p52 were transiently expressed in HCT116 cells, followed by knockdown of PIR with sh PIR and further reconstitution of PIR with rHA‐PIR. Chromatin immunoprecipitation (ChIP) was then performed to determine the binding of p52/RELB to FAS promoter using anti‐Flag antibody. H) HCT116 cells were transfected with Flag‐RELB and Flag‐p52, followed by treatment with DMSO or TphA (50 µm, 12 h). ChIP assays were then performed as in (G). I) HCT116 cells were firstly expressed for sh GFP (as control) and sh NFκB2 individually, to create cell lines with <t>NFκB1</t> knockdown. 24 hours later, each group of cells were further expressed for sh GFP or sh PIR ‐1. After cultured for another 72 hours, cells were evaluated for survival rate by flow cytometry (upper panel) and expression levels of indicated proteins by WB (lower panel). Data represent the mean±SD. (n = 5, unpaired Student's t ‐test, ** p < 0.01, *** p < 0.001). J) HCT116 cells were transfected with indicated plasmids. After 72 hours of transfection, cells were determined for survival rate (upper panel) and expression levels of proteins (lower panel). Date represents the mean±SD. (n = 4, unpaired Student's t ‐test, *** p < 0.001). K) HEK293T cells were co‐transfected with luciferase reporter plasmids carrying FAS full‐length promoter ( FAS ‐Luc) or its 3′‐2a deletion mutants ( FAS ‐3′‐2a‐Luc), along with different doses of Flag‐p52/RELB complex. After 24 hours of transfection, luciferase activities were determined and normalized to the first column (upper panel). Protein levels were determined by WB (lower panel). Data are presented as mean±SD. (n = 3, unpaired Student's t ‐test, ** p < 0.01, *** p < 0.001, n.s.: no significant difference).
GS
PROT-C
CO-A
Co-IP
KD
A Molecular docking predicts binding of EpOME to PLEC; B , C CETSA showed a right forward shift in thermal stability of PLEC upon the treatment of 12(13)-EpOME, indicating a direct binding of EpOME to PLEC in MDA-MB-231 cells; D Knockdown of PLEC downregulated CXCL9, but sh CXCL9 impacts PLEC slightly; E <t>NFκB1</t> was proposed as the transcription factor for CXCL9 , CXCL10 , and SAA1 by the hTFtarget database; F Co-IP analysis indicated the interaction between PLEC and NFκB1; G NFκB1 consensus motif relative to CXCL9 complementary region, TSS, transcription start site; H A ChIP assay revealed NFκB1 as the transcriptional factor of CXCL9 . Data are presented as Mean ± SD. Statistical difference (*** P < 0.001, ** P < 0.01, * P < 0.05) was determined by the Student t -test.
BIND-A
KD
Co-IP
×
91 / 100
Bioz Stars
Anti NFκB1
Cell Signaling Technology  
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Techniques
 
Article Snippets
"Antibodies used were anti-FOXM1 (sc-500; Santa Cruz Biotechnology), NFκB1 (#3035; Cell Signaling Technology), β-actin (H-196; Santa Cruz) and α-tubulin (#3873; Cell Signaling)."  (see more)
90 / 100
Bioz Stars
Nfκb1
Thermo Fisher Scientific / Applied Biosystems  
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Techniques
 
Article Snippets
"RNA concentrations and purity were assessed with the NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was evaluated with the 2100 Bioanalyzer system (Agilent Technologies, Wilmington, DE, USA) using the Agilent RNA 6000 .."  (see more)
80 / 100
Bioz Stars
NFκB1
Proteintech  
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Techniques
 
Article Snippets
".. : 1000, Abcam), p-IKBα S36 (ab133462, diluted 1 : 1000, Abcam), NFκB1 (14220-1-AP, diluted 1 : 1000, Proteintech), NFκB P65 (P65, ab32536, diluted 1 : 1000, Abcam), NFκB p-P65 .."  (see more)
85 / 100
Bioz Stars
NFκB1
QIAGEN / SABiosciences  
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Techniques
 
Article Snippets
"Analyzing the promoter region of CSF3 using a search engine based on SABiosciences' text mining application and the UCSC genome browser ( http://www.sabiosciences.com/chipqpcrsearch.php ), we identified putative binding sites for several transcription factors .."  (see more)
80 / 100
Bioz Stars
NFκB1
Cloud-Clone  
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Techniques
 
Article Snippets
".. blocked using BSA (5%) or skimmed milk (5%) in TBST and probed against NFκB1 (Cloud-Clone Corp), pP-65, (Thermo Fisher Scientific, USA), Caspase 3, Caspase 9, Bax, Cytochrome C, pcMyc, .."  (see more)
ACTIV
WB
EXP
QUANT-A
IF
91 / 100
Bioz Stars
NFκB1 p105
Revvity / Dharmacon  
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Techniques
 
Article Snippets
".. UL144 by siRNA was achieved using three custom designed siRNA primers (Dharmacon) with the following sequences: The siRNA for NFκB1 p105, NFκB p100 and TRAF2 were purchased from Qiagen."  (see more)
91 / 100
Bioz Stars
NFκB1
ABclonal Biotech  
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Techniques
 
Article Snippets
".. MN, USA), IκBα (A11397, ABclonal, Woburn, MA, USA), p-IκBα (AP0999, ABclonal, Woburn, MA, USA), NFκB1 (A6667, ABclonal, Woburn, MA, USA), p-NFκB1 (AP0125, ABclonal, Woburn, MA, USA), p65 (A19653, ABclonal, Woburn, MA, .."  (see more)
91 / 100
Bioz Stars
P-NFκB1
ABclonal Biotech  
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Techniques
 
Article Snippets
".. MA, USA), p-IκBα (AP0999, ABclonal, Woburn, MA, USA), NFκB1 (A6667, ABclonal, Woburn, MA, USA), p-NFκB1 (AP0125, ABclonal, Woburn, MA, USA), p65 (A19653, ABclonal, Woburn, MA, USA), p-p65 (AP0215."  (see more)
85 / 100
Bioz Stars
Anti NFκB1
Boster Bio  
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Techniques
 
Article Snippets
".. PHD-2 (1:1000, Rabbit monoclonal, Cell Signaling Technology Inc., Danvers, MA, USA); NFκB1 (p50, 1:500, Rabbit polyclonal, Boster, Wuhan, China); RelA (p65, 1:500, Rabbit polyclonal, Boster, Wuhan, China) and β-tubulin .."  (see more)
91 / 100
Bioz Stars
NFκB1 antibody
Cell Signaling Technology  
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Techniques
 
Article Snippets
"Chromatin immunoprecipitations were performed using NFκB1 antibody (Cell Signaling, #13586, 1:50) while Histone H3 and IgG antibodies were used as .."  (see more)