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All (2)
Zeiss
Thermo Fisher
 

About 11 products / 173 snippets / 3 images

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Bioz Stars
LSM 700 Inverted Laser Confocal Microscope
Zeiss  
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Techniques
 
Article Snippets
".. ProLongDiamondAntifadeReagent (ThermoFisher Scientific) and imaged with an LSM 700 Inverted Laser Confocal Microscope (Carl Zeiss GmbH, Oberkochen, Germany)."  (see more)
96 / 100
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LSM 700 inverted laser confocal microscope
Zeiss  
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Catalog#: 490040-0023-000
Techniques
Microscopy, Software, Staining, Incubation, Fluorescence, Imaging (see more)
Article Snippets
"The slides were mounted with a ProLong Diamond antifade reagent (Thermo-Fisher) and imaged with an LSM 700 inverted laser confocal microscope (Carl Zeiss, Oberkochen, Germany)."  (see more)
Expression and localization of G. lamblia PLK1 (GlPLK) in G. lamblia -expressing hemagglutinin (HA)-tagged GlPLK. a A schematic diagram of plasmid pGlPLK.neo. HA-tagged GlPLK was expressed from its own promoter, P glplk . Transfected trophozoites were selected by neomycin resistance conferred by the neo gene expressed by the P glggi promoter, a strong promoter of the γ-giardin gene. As a control, Giardia trophozoites were also transfected with pKS-3HA.neo, a vector control. b Western blotting to examine the expression of HA-tagged GlPLK. Extracts were prepared from G. lamblia containing empty vector (lane 1) or pGlPLK.neo (lane 2) and incubated with monoclonal mouse anti-HA antibodies. Membranes were first incubated in stripping buffer and then reacted with polyclonal rat antibodies specific to protein disulfide isomerase 1 (PDI1) of G. lamblia . c Localization of GlPLK. Giardia lamblia expressing HA-tagged GlPLK was probed with mouse anti-HA antibodies. The cells were then incubated with Alexa Fluor 488-conjugated anti-mouse IgG. Slides were mounted with ProLong™ Gold Antifade Mountant with the fluorescent stain DAPI, and then examined with a Zeiss <t>LSM700</t> inverted confocal laser scanning microscope. Scale bars: 2 µm. d Co-localization of GlPLK and α-tubulin in G. lamblia . Giardia cells expressing HA-tagged GlPLK were probed with rat anti-HA antibodies and mouse anti-acetylated-α-tubulin monoclonal antibodies. e Co-localization of GlPLK and G. lamblia centrin (GlCentrin) in G. lamblia . Cells were reacted with mouse anti-HA antibodies and rat anti-GlCentrin polyclonal antibodies. Cells were then incubated with Alexa Fluor 555-conjugated anti-rat IgG and Alexa Fluor 488-conjugated anti-mouse IgG. A differential interference contrast image was acquired to show cell morphology. Scale bars: 2 μm. DIC Differential interference contrast
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Expression and localization of truncated GlPLKs in G. lamblia . a A schematic diagram of plasmids pGlPLKKDL.neo and pGlPLKPBD.neo. Two truncated GlPLK proteins are expressed in an HA-tagged form from their own promoter, P glplk . Plasmid pGlPLKKDL encodes GlPLK with the KD and linker region, whereas pGlPLKPBD contains DNA coding for the polo-box domains (PBDs) of GlPLK. Plasmid pKS-3HA.neo was transfected into Giardia trophozoites as a control. b Western blotting to examine the expression of HA-tagged truncated GlPLKs. Extracts were prepared from G. lamblia containing empty vector (lane 1), pGlPLKKDL.neo (lane 2), or pGlPLKPBD.neo (lane 3), and incubated with monoclonal mouse anti-HA antibodies. Membranes were incubated in stripping buffer, and then reacted with polyclonal rat antibodies specific to GlPDI1. c Co-localization of GlPLK-KDL with α-tubulin ( i ) or GlCentrin ( ii , iii ). Giardia lamblia cells expressing HA-tagged truncated GlPLK-KDL were probed with rat anti-HA antibodies and mouse anti-acetylated-α-tubulin monoclonal antibodies. Otherwise, these cells were reacted with rat anti-GlCentrin polyclonal antibodies instead of anti-acetylated-α-tubulin monoclonal antibodies. Panel iii is an extended view of panel ii . Incorrectly positioned basal bodies are indicated with white arrows. d Co-localization of GlPLK-PBD with α-tubulin ( i ) or GlCentrin ( ii , iii ). G. lamblia cells expressing HA-tagged truncated GlPLK-PBD were probed with rat or mouse anti-HA antibodies along either with mouse anti-acetylated-α-tubulin antibodies ( i ), or rat anti-GlCentrin polyclonal antibodies ( ii , iii ), respectively. Panel iii is an extended view of panel ii . The cells were then incubated with Alexa Fluor 488-conjugated anti-rat IgG and Alexa Fluor 568-conjugated anti-mouse IgG (α-tubulin co-localization) or Alexa Fluor 555-conjugated anti-rat IgG and Alexa Fluor 488-conjugated anti-mouse IgG (for centrin co-localization). Slides were mounted with ProLong™ Gold Antifade Mountant with DAPI, and then examined with a Zeiss <t>LSM700</t> inverted confocal laser scanning microscope. A differential interference contrast image was acquired to show cell morphology. Scale bars: 2 μm
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Expression and localization of phosphorylated GlPLK in G. lamblia . a Giardia lamblia cells expressing HA-tagged GlPLK in the interphase (lane 1), G1/S phase (lane 2), and G2/M phase (lane 3). Western blot analysis. Extracts of these cells were probed with anti-phospho-PLK, anti-HA, anti-GlPLK or anti-GlPDI1 antibodies. Levels of phospho-GlPLK-HA, phospho-GlPLK, GlPLK-HA and GlPLK were normalized to GlPDI1, a protein loading control. Amounts of these GlPLK proteins are expressed as relative values to those observed in the interphase cells. The presented western blot is a representative of three independent experiments, and averages of three experiments are presented as a bar graph. b Localization of GlPLK and phosphorylated GlPLK in Giardia . G. lamblia expressing HA-tagged GlPLK were incubated with antibodies specific to the phosphorylated form of PLK (1:100) along with anti-HA antibodies. These cells were then reacted with anti-Alexa Fluor 568-conjugated anti-mouse IgG and Alexa Fluor 488-conjugated anti-rat IgG. Slides were mounted with ProLong™ Gold Antifade Mountant with DAPI, and then examined with a Zeiss <t>LSM700</t> inverted confocal laser scanning microscope. Scale bars: 2 µm
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85 / 100
Bioz Stars
LSM 700 inverted laser confocal microscope
Thermo Fisher Scientific / Invitrogen  
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Techniques
 
Article Snippets
"Following attachment, cells were treated with Zinc20267861, Zinc18204217, and Zinc33254827 or amlodipine for 10 min. Then, Fura-2-acetoxymethyl ester (Fura-2AM; F1201, Invitrogen, Thermo Fisher Scientific) at 1 μg/ml was added to the culture medium, and imaging .."  (see more)
97 / 100
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Confocal laser inverted microscope LSM700
Zeiss  
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Techniques
 
Article Snippets
"Images were acquired using a confocal laser inverted microscope LSM700 (Carl Zeiss, Oberkochen, Germany)."  (see more)
80 / 100
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LSM 700 laser scanning inverted confocal microscope
Zeiss  
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Techniques
 
Article Snippets
".. in homemade chambers built as previously described ( ) with a Zeiss LSM 700 laser scanning inverted confocal microscope controlled by the manufacturer’s software."  (see more)
80 / 100
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LSM 700 inverted laser-scanning confocal fluorescence microscope
Zeiss  
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Techniques
 
Article Snippets
".. Tokai Hit, Hamamatsu, Shizuoka, Japan), equipped with a Carl Zeiss LSM 700 inverted laser-scanning confocal fluorescence microscope."  (see more)
80 / 100
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LSM 700 confocal laser canning inverted fluorescence microscope
Zeiss  
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Techniques
 
Article Snippets
".. placed on microscope slides, ounted with coverslips, and observed under an LSM 700 confocal laser canning inverted fluorescence microscope (Carl Zeiss)."  (see more)
80 / 100
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LSM 700 inverted laser-scanning confocal 114 microscope
Zeiss  
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Techniques
 
Article Snippets
"All confocal images were acquired using a Zeiss LSM 700 inverted laser-scanning confocal 114 microscope."  (see more)
80 / 100
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Observer Z1 inverted LSM 700 Confocal Laser Scanning Microscope
Zeiss  
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Techniques
 
Article Snippets
"Digital confocal micrograph images were acquired on a Zeiss Observer Z1 inverted LSM 700 Confocal Laser Scanning Microscope (Zeiss, Germany)."  (see more)
80 / 100
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LSM T-PMT/LSM700 confocal laser scanning inverted microscope
Zeiss  
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Techniques
 
Article Snippets
"A Zeiss LSM T-PMT/LSM700 confocal laser scanning inverted microscope was used, samples were excited at 488 nm and fluorescence gathered using .."  (see more)
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LSM 700 inverted Axio Observer.Z1 confocal laser scanning microscope
Zeiss  
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Techniques
 
Article Snippets
".. transgenic plants was investigated using a Zeiss LSM 700 inverted Axio Observer.Z1 confocal laser scanning microscope (Carl Zeiss Microscopy) equipped with LD C-Apochromat water immersion objective .."  (see more)