We use essential cookies to operate our site. With your consent, we may also use non-essential cookies to improve your user experience and to analyze website traffic. Click "Accept" to accept cookies and go directly to the site, click "Reject" to reject all but cookies strictly necessary for the functioning of this site. You can reset your cookie settings at any time by visiting your Bioz "my account" page and selecting the "reset cookie preferences" link.

Accept Reject

All (29)
Cytoskeleton
MilliporeSigma
Cell Biolabs
SLIT2
Cell Signaling
Upstate Biotech
NewEast Bio
Thermo Fisher
21 More...
 

About 149 products / 752 snippets / 204 images

96 / 100
Bioz Stars
Cdc42 Pull-down Activation Assay Biochem Kit
Cytoskeleton  
Catalog#: BK034
Techniques
Activation Assay, Activity Assay, Western Blot, Incubation, Binding Assay (see more)
Article Snippets
"Methods: In cultured human umbilical vein endothelial cells (HUVEC), the effects of thrombin (0.2 U/ml) and insulin (0.1 IU/ml) on monolayer permeability (albumin flux), RhoA, Rac1 and cdc42 activation (pull down assay), actin cytoskeleton and VE-cadherin (confocal .."  (see more)
96 / 100
Bioz Stars
Cdc42 Pull-down Activation Assay Biochem Kit
Cytoskeleton  
star_border
Catalog#: BK034
Techniques
Activation Assay, Activity Assay, Western Blot, Incubation, Binding Assay (see more)
Article Snippets
"Methods: In cultured human umbilical vein endothelial cells (HUVEC), the effects of thrombin (0.2 U/ml) and insulin (0.1 IU/ml) on monolayer permeability (albumin flux), RhoA, Rac1 and cdc42 activation (pull down assay), actin cytoskeleton and VE-cadherin (confocal .."  (see more)
95 / 100
Bioz Stars
PAK-PBD beads - binds active Rac Cdc42 proteins
Cytoskeleton  
star_border
Catalog#: PAK02
Techniques
Incubation, Western Blot, Lysis, Binding Assay, Transfection, Software (see more)
Article Snippets
"The resulting supernatant was reacted with PAK-PBD (p21-activated kinase binding domain) (Cytoskeleton, Denver, CO) beads in binding buffer (25 mM Tris, pH 7.5, 30 .."  (see more)
200+  more images
Par6 and ECT2 activate <t>Cdc42</t> and PKCζ. (A) (Left panel) HeLa cells transfected with Myc-ECT2-ΔN5 together with Flag-Par6C vector or vector alone were lysed, and GTP-bound Cdc42 was pulled down with GST-PBD beads. The Western blot was carried out with anti-Cdc42 antibody. (Right panel) Similar experiments were performed in MDCK cells except that Flag-ECT2-ΔN5 was used. Accumulation of GTP-bound Rac1 was also estimated (lower panel). Similar results were obtained in three independent experiments. (B) Silencing of ECT2 reduces GTP-bound Cdc42. HeLa cells were treated with ECT2 siRNA or control luciferase siRNA (co) for 5 h and then transfected with Flag-Par6C. Cells were lysed 20 h after transfection, and GTP-bound Cdc42 was pulled down with GST-PBD-Sepharose beads. Western blots were carried out with anti-Cdc42, anti-ECT2, or anti-Flag antibody. These results were reproduced in three independent experiments. (C) ECT2 stimulates PKCζ activity. Cos7 cells transfected with the HA-tagged PKCζ expression vector in combination with the ECT2 and Flag-Par6C vectors or vector alone were lysed, and HA-PKCζ was immunoprecipitated with anti-HA antibody. PKCζ activity was estimated by in vitro kinase assays with myelin basic protein as the substrate, followed by autoradiography (upper panel). The level of HA-PKCζ was detected by immunoblotting with anti-HA antibody (lower panel). X-ray-films were scanned, and signals were quantitated with NIH Image software. Data are representative of three independent experiments.
TNSF
PLASMID-P
WB
CON
LUCIF
Na v 1.5 and V m <t>regulate</t> <t>Rac1</t> activation/distribution. (A) Images of representative cells after treatment with TTX (30 µM) and NS-1619 (1 µM) for 3 h. Cells were labeled with Rac1-GTP antibody (green), phalloidin (red) and DAPI (blue). Arrows in the Rac1-GTP panels highlight the distribution or lack of expression at the leading edge. (B) Rac1-GTP signal density, measured across 20 arcs, in 0.43 µm radius increments, within a quadrant mask region of interest at the leading edge, normalized to the first arc (n ≥ 66). (C) Peak Rac1-GTP signal density per cell from (B), normalized to the first arc (n ≥ 66). (D) Total Rac1-GTP quantified in whole cell lysates using colorimetric small <t>GTPase</t> activation assay (n = 6). (E) Images of representative cells after treatment with TTX (30 µM) and NS-1619 (1 µM) for 3 h. Cells were labeled with Rac1-GTP antibody (green), total Rac1 antibody (red), and DAPI (blue). Arrows in the Rac1-GTP panels highlight the distribution or lack of expression at the leading edge. (F) Total Rac1 signal density, measured across 20 arcs, in 0.43 µm radius increments, within a quadrant mask region of interest at the leading edge, normalized to the first arc (n ≥ 59). (G) Peak Rac1 signal density per cell from (F), normalized to the first arc (n ≥ 59). (H) Ratio of Peak Rac1-GTP/Peak total Rac1 for each experimental repeat, normalized to control (n = 3). Data are mean and SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ANOVA with Tukey test.
ACTIV
LBLNG
EXP
CON
A) IPSC-derived neurons transduced with LAMP1-mCh (red) were treated with DMSO (vehicle control), NAV-2729 (Arf6 inhibitor), EHT 1864 (Rac1 inhibitor), ML 141 <t>(Cdc42</t> inhibitor) or Rhosin (RhoA inhibitor). After treatment, neurons were incubated with Aβ42o (red) for 30 min the imaged live. Colocalization between Aβ42o and LAMP1 was quantitated with Imaris 10.1 (Bitplane) software to determine uptake of oligomers to lysosomes (white). B) Quantification of the mean percent of Aβ42o signal colocalized with LAMP1 from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Differences in colocalization between DMSO, NAV-2729, EHT 1864, ML 141, and Rhosin were assessed using a one-way ANOVA with Tukey’s test. C) Neurons treated with DMSO, NAV-2729, EHT 1864, ML 141, and Rhosin were incubated with tauPFFs (green) for 30 min, then imaged lived. Colocalization between tauPFFs and LAMP1-labeled lysosomes (white pixels) was measured. D) Quantification of the mean percent of tauPFF colocalized with LAMP1 from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Differences between each treatment were quantitated using a one-way ANOVA with Tukey’s test. Data is presented as mean ± SEM. * p<0.05. Scale bar = 10μm .
DER-A
TRANSDUC
CON
INCU
SOFT
A – The effect of AIBP (0.4 μg/ml, 2 h) on the abundance of F-actin in THP-1 cells. Panels on the right show higher magnification of the designated area from the left panels, arrows point to the microfilament-like F-actin structures (LifeAct staining, confocal microscopy). Scale bars left panels – 20 μm, right panels - 5 μm. B – Quantitation of the effect of AIBP (0.4 μg/ml, 2 h) on the abundance of F-actin by confocal microscopy (LifeAct staining, mean fluorescence intensity). Mean ± SEM are shown, *p<0.05, n=6. C - The effect of AIBP (0.4 μg/ml, 4 h) on the abundance of total <t>CdC42</t> and phosphorylated Cdc42 in THP-1 cells (Western blot). D – Quantitation of the effect of AIBP on the abundance of total CdC42 in THP-1 cells (densitometry of Western blot). *p<0.05, n=5. E - The abundance of total CdC42 after treatment of THP-1 cells with siRNACdc42 (Western blot). F – The abundance of [3H]cholesterol in lipid raft fractions after isolation of rafts by sequential gradient centrifugation from THP-1 cells untreated or treated with AIBP (0.4 μg/ml, 4 h) with or without Cdc42 silencing. Mean ± SEM are shown, **p<0.01 versus no AIBP, #p<0.05 versus siRNAscram; n=3.
STAIN
CONF
QUANT-A
FLUOR
WB
×
95 / 100
Bioz Stars
Cdc42 G-LISA GTPase Activation Assay
Cytoskeleton  
star_border
Catalog#: BK127
Techniques
Activation Assay, Activity Assay, Software, Incubation, Lysis (see more)
Article Snippets
".. cell lysates using a colorimetric ELISA-based small GTPase activation assay, according to the manufacturer’s instructions (G-LISA; Cytoskeleton, Inc) ( )."  (see more)
ACTIV
LBLNG
EXP
CON
94 / 100
Bioz Stars
Rac and Cdc42 Activator
Cytoskeleton  
star_border
Catalog#: CN02
Techniques
 
Article Snippets
"al growth factor (EGF; Cytoskeleton Inc., Cat# CN02) at 0,5 units/mL for 5 min. Cells were then fixed by "  (see more)
90 / 100
Bioz Stars
PAK1/CDC42, Active
SignalChem  
star_border
Catalog#: P02-10G
Techniques
 
Article Snippets
"and purified as described previously. 34 Recombinant mouse PAK1 and Cdc42/PAK1 were purchased from SignalChem (Richmond, BC, Canada) and recombinant Rac1 from Cytoskeleton (Denver, CO, USA). Recombina"  (see more)
90 / 100
Bioz Stars
CDC42, Active
SignalChem  
star_border
Catalog#: C108-310GH
Techniques
 
Article Snippets
"and purified as described previously. 34 Recombinant mouse PAK1 and Cdc42/PAK1 were purchased from SignalChem (Richmond, BC, Canada) and recombinant Rac1 from Cytoskeleton (Denver, CO, USA). Recombina"  (see more)
94 / 100
Bioz Stars
Rho Rac Cdc42 Activator I
Cytoskeleton  
star_border
Catalog#: CN04
Techniques
Activation Assay, Incubation, Activity Assay, Control, Cell Culture (see more)
Article Snippets
"RhoA activity was stimulated with 10% FBS and 0.25 μg/mL ACT (Rho activator II; CN04; Cytoskeleton) for 3 hours."  (see more)
95 / 100
Bioz Stars
RhoA Rac1 Cdc42 Activation Assay Combo Biochem Kit - bead pull-down format
Cytoskeleton  
star_border
Catalog#: BK030
Techniques
 
Article Snippets
"ochem Kit (bead pull-down format) Cytoskeleton BK030 G/F actin kit Cytoskeleton BK037 Mouse Neuron "  (see more)
94 / 100
Bioz Stars
Active Cdc42 Detection Kit
Cell Signaling Technology  
star_border
Catalog#: 8819
Techniques
Western Blot, Activation Assay, Incubation, Lysis, Pull Down Assay (see more)
Article Snippets
"Antibodies against p65 (Cat:8242), p-p65 (Cat:3033), the Active Cdc42 Detection Kit (Cat: 8819) were purchased from Cell Signaling Technology (Massachusetts, USA)."  (see more)
94 / 100
Bioz Stars
RhoA Rac1 Cdc42 G-LISA GTPase Activation Assay Bundle
Cytoskeleton  
star_border
Catalog#: BK135
Techniques
Activation Assay, Enzyme-linked Immunosorbent Assay, Isolation (see more)
Article Snippets
".. measured using a Cdc42 G-LISA activation assay kit according to manufacturer ́s instructions (BK135, Cytoskeleton, USA)."  (see more)
80 / 100
Bioz Stars
Cdc42 activation
SLIT2  
star_border
Techniques
 
Article Snippets
"Slit2 downregulates Cdc42 activation and cell motility by affecting the Robo1-srGAP1 and Robo1-Arl4A associations A previous .."  (see more)
85 / 100
Bioz Stars
Active Cdc42
Addgene  
star_border
Techniques
 
Article Snippets
"The retroviral vector of constitutively active Cdc42 (pMX GFP Cdc42Q61L) was purchased from Addgene."  (see more)