Journal: eLife

Article Title: Persistent epigenetic memory impedes rescue of the telomeric phenotype in human ICF iPSCs following DNMT3B correction

doi: 10.7554/eLife.47859

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , DNA amplification using FastStart Taq DNA Polymerase, dNTPack , MERCK, Darmstadt, Germany , Cat. #: 04738314001 , .

Techniques: Western Blot, Recombinant, Plasmid Preparation, CRISPR, Clone Assay, Sequencing, Protease Inhibitor, Purification, Reverse Transcription, Modification, DNA Amplification, Staining, SYBR Green Assay, Software

Journal: eLife

Article Title: Persistent epigenetic memory impedes rescue of the telomeric phenotype in human ICF iPSCs following DNMT3B correction

doi: 10.7554/eLife.47859

Figure Lengend Snippet:

Article Snippet: After the conversion, DNA was amplified using FastStart Taq DNA Polymerase, dNTPack (04738314001, MERCK, Darmstadt, Germany) with the following program: 95°C for 5 min; 4 cycles of 95°C for 1 min, 53°C for 3 min, 72°C for 3 min; 2 cycles of 95°C for 30 s, 55°C for 45 s, 72°C for 45 s; 40 cycles of 95°C for 30 s, 72°C for 1.5 min; 72°C for 10 min. PCR products were purified by QIAquick PCR purification kit (28104, QIAGEN, MD, USA).

Techniques: Western Blot, Recombinant, Plasmid Preparation, CRISPR, Clone Assay, Sequencing, Protease Inhibitor, Purification, Reverse Transcription, Modification, DNA Amplification, Staining, SYBR Green Assay, Software