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fluidigm dntpack
Dntpack, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dntpack/product/fluidigm
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
dntpack - by Bioz Stars, 2020-07
93/100 stars

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Polymerase Chain Reaction:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR data The high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Activation Assay:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR data The high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Amplification:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR data The high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Chromatin Immunoprecipitation:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR data The high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

Concentration Assay:

Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
Article Snippet: .. The following exceptions were considered: (i) Activation, elongation and final elongation were adjusted to fit each enzyme's recommended protocol. (ii) Annealing temperature from the sixth amplification step and on was according to each enzyme's elongation temperature. (iii) PCR reaction was stopped when amplification reached a plateau. (iv) Due to failure of dNTPack to amplify using the standard two-step PCR protocol, we applied the same program as being performed in the first PCR of T3 (in the AA chip). (v) Reactions mixes were according to manufacturer's protocols, with primer concentrations of 0.3–0.5 μM, with the exception of dNTPack, which composition was according to Fluidigm's recommended reaction mixture with primer concentration of 0.1 μM each and a final volume of 10.6 μl. .. Experimental validation of the model by using single cell STR data The high fidelity PCR enzymes were used in this study were: NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB), NEBNext Ultra II Q5 Master Mix (NEB), FastStart High Fidelity PCR System, dNTPack (Roche), KOD Hot Start DNA Polymerase (Novagen), KAPA HiFi HotStart PCR Kit (Kapa Biosystems) and PrimeStar Max (Takara).

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  • 93
    fluidigm dntpack
    Dntpack, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntpack/product/fluidigm
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dntpack - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    fluidigm dntpack s
    Dntpack S, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dntpack s/product/fluidigm
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dntpack s - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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