dntpack  (Millipore)


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    Name:
    FastStart High Fidelity PCR System
    Description:
    The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready to use solution of PCR grade nucleotides The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified This protein mediates proofreading activity but carries no polymerase activity Both proteins are inactive below 75C and are activated by heating to 95C for two minutes The FastStart R High Fidelity PCR System is the ideal tool for all amplification experiments where a combination of high specificity sensitivity accuracy and yield is needed Hot start protocols have improved specificity sensitivity and PCR yield Heat labile blocking groups on amino acid residues of FastStart Taq DNA Polymerase make the enzyme inactive at room temperature There is no elongation at low temperatures when primers bind nonspecifically
    Catalog Number:
    fhifin-ro
    Price:
    None
    Applications:
    The FastStart(R) High Fidelity PCR System - an optimized enzyme blend for hot start PCR - combines all the benefits of FastStart Taq DNA Polymerase with fourfold higher accuracy and the ability to amplify fragments up to 5kb.. Hot start PCR. Multiplex PCR. Hot Start RT-PCR. GC-rich template amplification. Difficult or challenging PCRsThe FastStart High Fidelity PCR System is the product of choice for multiplex PCR.
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    Structured Review

    Millipore dntpack
    FastStart High Fidelity PCR System
    The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready to use solution of PCR grade nucleotides The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified This protein mediates proofreading activity but carries no polymerase activity Both proteins are inactive below 75C and are activated by heating to 95C for two minutes The FastStart R High Fidelity PCR System is the ideal tool for all amplification experiments where a combination of high specificity sensitivity accuracy and yield is needed Hot start protocols have improved specificity sensitivity and PCR yield Heat labile blocking groups on amino acid residues of FastStart Taq DNA Polymerase make the enzyme inactive at room temperature There is no elongation at low temperatures when primers bind nonspecifically
    https://www.bioz.com/result/dntpack/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dntpack - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Microbial colonization is required for normal neurobehavioral development in zebrafish
    Article Snippet: .. The 16S rRNA gene was amplified in triplicate PCR reactions using the Roche FastStart High Fidelity PCR System (Sigma-Aldich, #4738292001). ..

    Article Title: DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber
    Article Snippet: .. Target amplification was performed using the FastStart High Fidelity PCR System, dNTPack (Sigma-Aldrich, Cat. 4738284001) in the 48.48 layout on the Fluidigm® C1 system (Fluidigm®, USA), a microfluidics platform. .. Library preparation was performed using the same kit including 4 μl of Access Array Barcode Library Primer and 1 μl of PCR product diluted 1:100.

    Article Title: Whole-genome characterization and resistance-associated substitutions in a new HCV genotype 1 subtype
    Article Snippet: .. To amplify these new fragments, we used the products of the external RT-PCRs obtained previously on the FastStart High Fidelity PCR System, dNTPack (#04738292001 Sigma, Roche), adapting the thermocycler programs to the characteristics of each different primer pair. .. Again all fragments were checked and purified on 1.5% agarose gel, and were deep-sequenced using the MiSeq Reagent Kit v3 (600 cycle) (#MS-102–3003, Illumina) according to a previously described method.

    Article Title: Whole-genome characterization and resistance-associated substitutions in a new HCV genotype 1 subtype
    Article Snippet: .. All primers were numbered according to isolate H77 (accession number AF009606) The external fragments were amplified using the Transcriptor One Step RT-PCR Kit (#04655885001 Sigma, Roche), and for the internals, the FastStart High Fidelity PCR System, dNTPack (#04738292001 Sigma, Roche), adapting the various PCR programs to the lengths and temperatures of each specific primer pair. .. Due to the high variability of the region corresponding to internal fragment number 11, a random priming (#11034731001 Sigma, Roche) PCR was necessary to perform between the RT-PCR and the nested PCR with the specific primers, to obtain enough DNA for analysis.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Whole-genome characterization and resistance-associated substitutions in a new HCV genotype 1 subtype
    Article Snippet: .. All primers were numbered according to isolate H77 (accession number AF009606) The external fragments were amplified using the Transcriptor One Step RT-PCR Kit (#04655885001 Sigma, Roche), and for the internals, the FastStart High Fidelity PCR System, dNTPack (#04738292001 Sigma, Roche), adapting the various PCR programs to the lengths and temperatures of each specific primer pair. .. Due to the high variability of the region corresponding to internal fragment number 11, a random priming (#11034731001 Sigma, Roche) PCR was necessary to perform between the RT-PCR and the nested PCR with the specific primers, to obtain enough DNA for analysis.

    Amplification:

    Article Title: Microbial colonization is required for normal neurobehavioral development in zebrafish
    Article Snippet: .. The 16S rRNA gene was amplified in triplicate PCR reactions using the Roche FastStart High Fidelity PCR System (Sigma-Aldich, #4738292001). ..

    Article Title: DNA methylation clocks as a predictor for ageing and age estimation in naked mole-rats, Heterocephalus glaber
    Article Snippet: .. Target amplification was performed using the FastStart High Fidelity PCR System, dNTPack (Sigma-Aldrich, Cat. 4738284001) in the 48.48 layout on the Fluidigm® C1 system (Fluidigm®, USA), a microfluidics platform. .. Library preparation was performed using the same kit including 4 μl of Access Array Barcode Library Primer and 1 μl of PCR product diluted 1:100.

    Article Title: Whole-genome characterization and resistance-associated substitutions in a new HCV genotype 1 subtype
    Article Snippet: .. All primers were numbered according to isolate H77 (accession number AF009606) The external fragments were amplified using the Transcriptor One Step RT-PCR Kit (#04655885001 Sigma, Roche), and for the internals, the FastStart High Fidelity PCR System, dNTPack (#04738292001 Sigma, Roche), adapting the various PCR programs to the lengths and temperatures of each specific primer pair. .. Due to the high variability of the region corresponding to internal fragment number 11, a random priming (#11034731001 Sigma, Roche) PCR was necessary to perform between the RT-PCR and the nested PCR with the specific primers, to obtain enough DNA for analysis.

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  • 84
    Millipore gc rich pcr system dntpack
    Gc Rich Pcr System Dntpack, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc rich pcr system dntpack/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gc rich pcr system dntpack - by Bioz Stars, 2020-07
    84/100 stars
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