Journal:

Article Title: Prevalence of, Antibody Response to, and Immunity Induced by Haemophilus ducreyi Hemolysin

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: E. coli BL21(DE3) Host for protein expression Novagen, Madison, Wis. H. aphrophilus ATCC 33389 Arnold Smith H. ducreyi 35000-TcA Strain 35000 with Tn 916 inserted in hhdB , nonhemolytic 50 H. ducreyi 35000-KmA Strain 35000 with Tn 1545-Δ3 inserted in hhdB , nonhemolytic 50 H. ducreyi 35000ΔAPC Strain 35000 with cat cassette in hhdA gene, nonhemolytic 54 H. haemoglobinophilus ATCC 19416 ATCC, Manassas, Va. H. haemolyticus ATCC 33390 Arnold Smith H. influenzae ATCC 33911 Arnold Smith H. influenzae Rd Marilyn Roberts H. parainfluenzae ATCC 33392 Arnold Smith H. paraphrophilus ATCC 29237 Arnold Smith H. segmis ATCC 33393 Arnold Smith T. equigenitalis ATCC 35865 Arnold Smith Plasmids pUC19 E. coli cloning vector, Ap r GibcoBRL pCR2.1 TA cloning vector for cloning PCR products, Amp r Kan r Invitrogen, San Diego, Calif. pET24+ Expression plasmid with C-terminal His tag sequence and inducible T7 promoter, no translation initiation signals, Kan r Novagen pET24a+ Expression plasmid with C-terminal His tag sequence and inducible T7 promoter, Kan r Novagen pBCKS Cm r plasmid derived from pUC19 with KS multiple cloning site Stratagene, La Jolla, Calif. pPT384-ETa hhdBA genes cloned into pET24a+ Sst I- Sal I This study pLSSK Shuttle vector with ori , sulA , and strA genes from pLS88 and multiple cloning sites and lacZ gene from pBluescript SK 54 pPT384 hhdBA gene region in pTZ18 in same orientation as lac promoter 50 pPT376BCKS 5.8-kb Bgl II fragment containing part of hhdB and all of hhdA cloned into pBCKS Sst I- Sal I 50 pETBABN hhdBA genes cloned into pET24+ Bam HI- Sal I This study pLSBAHis+ hhdBA genes cloned into pLSSK in same orientation as lac promoter.

Techniques: Plasmid Preparation, Cloning, Expressing, TA Cloning, Sequencing, Derivative Assay, Clone Assay

Journal: Journal of Bacteriology

Article Title: TetR Family Transcriptional Regulator PccD Negatively Controls Propionyl Coenzyme A Assimilation in Saccharopolyspora erythraea

doi: 10.1128/JB.00281-17

Figure Lengend Snippet: Bacterial strains and plasmids used in this work

Article Snippet: Next, about 0.5 to 1 ml of the seed culture was added to a 500-ml flask containing 50 ml TSB (initial optical density at 600 nm [OD 600 ] = 0.05) or minimal Evans medium ( 36 ) (initial OD 600 = 0.1) supplemented with various carbon sources grown at 30°C and 200 rpm for genomic DNA extraction, phenotype, or transcription studies. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Characteristic(s) Source or reference Strains S. erythraea NRRL2338 Used as parental strain, wild type DSM 40517 Δ pccD strain S. erythraea pccD null mutant, thiostrepton resistance This work WT/PIB- pccD pccD overexpression strain, WT carrying pIB- pccD This work E. coli Rosetta (DE3) F − ompT hsdS B (r B − m B − ) gal dcm λ(DE3)pRARE 2 (Cam r ) Novagen Plasmids pET19b Expression vector, Amp r Novagen pET- pccD pET19b derivative carrying pccD This work pMD-18T TA-cloning vector TaKaRa pUC19-tsr pUC18 derivative containing a 1.36-kb fragment of a thiostrepton resistance cassette in the BamHI/SmaI sites 34 pUC- pccD pUC19-tsr, with the 1.5-kb DNA fragments upstream and downstream of pccD gene inserted upstream and downstream of tsr correspondingly This work pIB139 E. coli-S. erythraea integrative shuttle vector containing a strong constitutive ermE * promoter, apramycin resistance 35 pIB- pccD pIB139 carrying an extra pccD for the gene overexpression This work Open in a separate window Bacterial strains and plasmids used in this work

Techniques: Plasmid Preparation, Mutagenesis, Over Expression, Expressing

Journal: Toxins

Article Title: Modified Heat-Stable Toxins (hSTa) of Enterotoxigenic Escherichia coli Lose Toxicity but Display Antigenicity after Being Genetically Fused to Heat-Labile Toxoid LT(R192G)

doi: 10.3390/toxins3091146

Figure Lengend Snippet: Escherichia coli strains and plasmids used in this study.

Article Snippet: Vector pUC19 (Promega, Madison, WI, USA) was used to clone and express the wildtype and mutated STa genes, and expression vector pET28α (Invitrogen, Carlsbad, CA, USA) was used to express LT and STa toxoid fusions.

Techniques: Plasmid Preparation, Construct, Recombinant, Negative Control