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Promega puc19
Msc I partial digestion of talC-Bam HI fragment cloned in <t>pUC19.</t> 2 μg plasmid DNA was digested with 5 u Msc I enzyme. The digestion times are given in minutes above the gel. M, 100 bp DNA ladder makers.
Puc19, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes"

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes

Journal: Scientific Reports

doi: 10.1038/srep13162

Msc I partial digestion of talC-Bam HI fragment cloned in pUC19. 2 μg plasmid DNA was digested with 5 u Msc I enzyme. The digestion times are given in minutes above the gel. M, 100 bp DNA ladder makers.
Figure Legend Snippet: Msc I partial digestion of talC-Bam HI fragment cloned in pUC19. 2 μg plasmid DNA was digested with 5 u Msc I enzyme. The digestion times are given in minutes above the gel. M, 100 bp DNA ladder makers.

Techniques Used: Clone Assay, Plasmid Preparation

Related Articles

Clone Assay:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ]. .. In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Article Title: Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins
Article Snippet: An E. coli -LAB shuttle vector, pLES003-b, which carries the AatII fragment reversed from pLES003 (DDBJ accession no. ) ( ) as the replication origin, was used for the promoter assay and the reconstruction of the brevicin 174A biosynthetic gene cluster. .. E. coli DH5α and the pUC19, pGEM-T (Promega), and pTA2 (Toyobo) plasmids were used for DNA cloning and sequencing. .. E. coli HST04 was used for the preparation of a Dam and/or Dcm methylation-free plasmid.

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: Phage 20422-1 DNA was purified from phage isolated following infections of L. monocytogenes DP-L862 using the Lambda miniprep phage extraction kit (Qiagen, Valencia, CA). .. The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs). .. The recombinant plasmids were transformed into competent Escherichia coli DH5α cells, and transformants were selected using ampicillin (100 μg/ml) and X-Gal (5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside).

Article Title: Identification, isolation, and analysis of a gene cluster involved in iron acquisition by Pseudomonas mendocina ymp
Article Snippet: Escherichia coli DH5 α (Invitrogen) and JM109 (Promega, Madison, WI) were routinely used as host strains for cloning DNA fragments. .. The pUC19 and pGEM-T vectors (Promega) were used for cloning DNA fragments. .. Restriction enzymes and DNA/gel purification kits were purchased from Promega and used according to the manufacturer’s instructions.

Article Title: The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
Article Snippet: When indicated, the growth medium was supplemented with yeast extract (5 mg/ml), thiamine (2 μg/ml), nicotinic acid (12.5 μg/ml), chloramphenicol (20 μg/ml), ampicillin (50 μg/ml), tetracycline (10 μg/ml), and kanamycin (Kn, 40 μg/ml). .. Plasmids pPK4071 and pPK4083 were made by digesting λ430 ( ) with Nru I and cloning this 5-kb fragment containing iscRSUAhscB ( ) into the Sma I site of pUC19 ( ) and pAlter-1 (Promega), respectively. .. Plasmids pPK4196 and pPK4194 were generated by cloning PCR products containing only the ORFs for iscS and iscSUA into pET11a , respectively (Fig. ).

Amplification:

Article Title: Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis
Article Snippet: The 5′ end of the gfp transcript, which is initiated by P3, was then amplified with random-primer reverse transcription and nested PCR using gfp specific primers with the provided primers in the kit. .. PCR products were then inserted into pUC19 (Promega, Madison, WI) for DNA sequencing.

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs). .. The recombinant plasmids were transformed into competent Escherichia coli DH5α cells, and transformants were selected using ampicillin (100 μg/ml) and X-Gal (5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside).

Article Title: The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
Article Snippet: Plasmids pPK4071 and pPK4083 were made by digesting λ430 ( ) with Nru I and cloning this 5-kb fragment containing iscRSUAhscB ( ) into the Sma I site of pUC19 ( ) and pAlter-1 (Promega), respectively. .. Plasmid pPK4344 was generated by cloning into pGem2 (Promega), a PCR product containing sequence beginning within the iscR ORF and ending within the iscA ORF (Fig. ).

Construct:

Article Title: Inhibition of Hepatitis B Virus Replication by MyD88 Involves Accelerated Degradation of Pregenomic RNA and Nuclear Retention of Pre-S/S RNAs
Article Snippet: Taken together, our results define a novel antiviral mechanism against HBV mediated by MyD88. .. To construct HBV plasmid pHBV1.3, a terminally redundant (1.3× copy), replication-competent HBV genome (subtype adw; nucleotides 957 to 1952; GenBank accession number ) was inserted into pUC19 (Promega). pCIdA-HBV expresses all the HBV proteins, but it lacks the 5′ ɛ RNA signal, which eliminates pregenomic RNA packaging and DNA replication ( ). .. Both pCMV-HBV ( ) and pTet-HBV ( ) contain the wild-type HBV 1.1-mer overlength genomic sequence.

Electrophoresis:

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs). .. The recombinant plasmids were transformed into competent Escherichia coli DH5α cells, and transformants were selected using ampicillin (100 μg/ml) and X-Gal (5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside).

Luciferase:

Article Title: Inhibition of Hepatitis B Virus Replication by MyD88 Involves Accelerated Degradation of Pregenomic RNA and Nuclear Retention of Pre-S/S RNAs
Article Snippet: To construct HBV plasmid pHBV1.3, a terminally redundant (1.3× copy), replication-competent HBV genome (subtype adw; nucleotides 957 to 1952; GenBank accession number ) was inserted into pUC19 (Promega). pCIdA-HBV expresses all the HBV proteins, but it lacks the 5′ ɛ RNA signal, which eliminates pregenomic RNA packaging and DNA replication ( ). .. For these plasmids, the synthesis of the pregenomic RNA is driven by the cytomegalovirus (CMV) promoter and the Tet promoter, respectively. pCMV-HBV M2 is a derivative of pCMV-HBV in which the La protein-binding sites have been mutated ( ). pCMV-HBVΔPRE is a PRE deletion mutant of pCMV-HBV ( ).

Activity Assay:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ]. .. Indeed, when as little as 50 bp of the SV40 enhancer was cloned into these other plasmids, they were able to enter nuclei with the same kinetics as the entire SV40 genome [ , ].

Over Expression:

Article Title: Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins
Article Snippet: E. coli BL21(DE3) and the pET-28a(+) plasmid (Novagen) were used for the overexpression of BreD and BreG gene products. .. E. coli DH5α and the pUC19, pGEM-T (Promega), and pTA2 (Toyobo) plasmids were used for DNA cloning and sequencing.

Derivative Assay:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ]. .. This finding supports earlier studies by Graessman [ ] in which the SV40 enhancer was postulated to have a ‘helper’ activity for nuclear localisation apart from the classical transcriptional activity attributed to it.

Hybridization:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water. .. The dot blot on colony was performed following the manual provided with the Amersham Hybond-N+ Membranes (GE Healthcare).

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: When protein-free, purified SV40 DNA (5243 bp) was microinjected into the cytoplasm of TC7 African Green monkey kidney cells, the majority of plasmids were detected in cell nuclei by 6 – 8 h after injection by in situ hybridisation. .. In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Ligation:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The resulting Bam HI fragments with sizes from 1.5 kb to 7.5 kb were recovered from agarose gel using the QIAquick Gel Extraction Kit (Qiagen). .. The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water. .. The ligations were performed at 4 °C for 12 h, and the ligation products were desalted using MFTM membrane filters (0.025 μm, VSWP, Millipore) before being electroporated into E. coli strain Trans5α.

Cell Culture:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ]. .. This finding supports earlier studies by Graessman [ ] in which the SV40 enhancer was postulated to have a ‘helper’ activity for nuclear localisation apart from the classical transcriptional activity attributed to it.

Generated:

Article Title: The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
Article Snippet: Plasmids pPK4071 and pPK4083 were made by digesting λ430 ( ) with Nru I and cloning this 5-kb fragment containing iscRSUAhscB ( ) into the Sma I site of pUC19 ( ) and pAlter-1 (Promega), respectively. .. Plasmids pPK4196 and pPK4194 were generated by cloning PCR products containing only the ORFs for iscS and iscSUA into pET11a , respectively (Fig. ).

other:

Article Title: Molecular cloning of rhodanese gene from soil metagenome of cold desert of North-West Himalayas: sequence and structural features of the rhodanese enzyme
Article Snippet: Escherichia coli strains JM110 and DH5α were procured from Stratagene (USA), while pUC19 was purchased from (Promega).

DNA Sequencing:

Article Title: Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis
Article Snippet: The 5′ end of the gfp transcript, which is initiated by P3, was then amplified with random-primer reverse transcription and nested PCR using gfp specific primers with the provided primers in the kit. .. PCR products were then inserted into pUC19 (Promega, Madison, WI) for DNA sequencing. .. C. trachomatis infected HeLa cells were harvested at indicated times post infection.

Polymerase Chain Reaction:

Article Title: Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis
Article Snippet: The 5′ end of the gfp transcript, which is initiated by P3, was then amplified with random-primer reverse transcription and nested PCR using gfp specific primers with the provided primers in the kit. .. PCR products were then inserted into pUC19 (Promega, Madison, WI) for DNA sequencing. .. C. trachomatis infected HeLa cells were harvested at indicated times post infection.

Article Title: The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
Article Snippet: Plasmids pPK4071 and pPK4083 were made by digesting λ430 ( ) with Nru I and cloning this 5-kb fragment containing iscRSUAhscB ( ) into the Sma I site of pUC19 ( ) and pAlter-1 (Promega), respectively. .. Plasmids pPK4196 and pPK4194 were generated by cloning PCR products containing only the ORFs for iscS and iscSUA into pET11a , respectively (Fig. ).

Injection:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: When protein-free, purified SV40 DNA (5243 bp) was microinjected into the cytoplasm of TC7 African Green monkey kidney cells, the majority of plasmids were detected in cell nuclei by 6 – 8 h after injection by in situ hybridisation. .. In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Promoter Assay:

Article Title: Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins
Article Snippet: An E. coli -LAB shuttle vector, pLES003-b, which carries the AatII fragment reversed from pLES003 (DDBJ accession no. ) ( ) as the replication origin, was used for the promoter assay and the reconstruction of the brevicin 174A biosynthetic gene cluster. .. E. coli DH5α and the pUC19, pGEM-T (Promega), and pTA2 (Toyobo) plasmids were used for DNA cloning and sequencing.

Mutagenesis:

Article Title: Inhibition of Hepatitis B Virus Replication by MyD88 Involves Accelerated Degradation of Pregenomic RNA and Nuclear Retention of Pre-S/S RNAs
Article Snippet: To construct HBV plasmid pHBV1.3, a terminally redundant (1.3× copy), replication-competent HBV genome (subtype adw; nucleotides 957 to 1952; GenBank accession number ) was inserted into pUC19 (Promega). pCIdA-HBV expresses all the HBV proteins, but it lacks the 5′ ɛ RNA signal, which eliminates pregenomic RNA packaging and DNA replication ( ). .. Both pCMV-HBV ( ) and pTet-HBV ( ) contain the wild-type HBV 1.1-mer overlength genomic sequence.

Isolation:

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: Phage 20422-1 DNA was purified from phage isolated following infections of L. monocytogenes DP-L862 using the Lambda miniprep phage extraction kit (Qiagen, Valencia, CA). .. The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs).

Purification:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The resulting Bam HI fragments with sizes from 1.5 kb to 7.5 kb were recovered from agarose gel using the QIAquick Gel Extraction Kit (Qiagen). .. The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water. .. The ligations were performed at 4 °C for 12 h, and the ligation products were desalted using MFTM membrane filters (0.025 μm, VSWP, Millipore) before being electroporated into E. coli strain Trans5α.

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: When protein-free, purified SV40 DNA (5243 bp) was microinjected into the cytoplasm of TC7 African Green monkey kidney cells, the majority of plasmids were detected in cell nuclei by 6 – 8 h after injection by in situ hybridisation. .. In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: Phage 20422-1 DNA was purified from phage isolated following infections of L. monocytogenes DP-L862 using the Lambda miniprep phage extraction kit (Qiagen, Valencia, CA). .. The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs).

Article Title: Identification, isolation, and analysis of a gene cluster involved in iron acquisition by Pseudomonas mendocina ymp
Article Snippet: Cosmid and plasmid DNA were manipulated and purified by standard techniques ( ). .. The pUC19 and pGEM-T vectors (Promega) were used for cloning DNA fragments.

Dot Blot:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water. .. The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water.

Sequencing:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: Paragraph title: 3.3 Sequence-specific nuclear import of plasmid DNA ... In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Article Title: Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins
Article Snippet: An E. coli -LAB shuttle vector, pLES003-b, which carries the AatII fragment reversed from pLES003 (DDBJ accession no. ) ( ) as the replication origin, was used for the promoter assay and the reconstruction of the brevicin 174A biosynthetic gene cluster. .. E. coli DH5α and the pUC19, pGEM-T (Promega), and pTA2 (Toyobo) plasmids were used for DNA cloning and sequencing. .. E. coli HST04 was used for the preparation of a Dam and/or Dcm methylation-free plasmid.

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: Paragraph title: Nucleotide sequence determinations of genomic fragments of phages 20422-1 and 805405-1. ... The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs).

Article Title: Identification, isolation, and analysis of a gene cluster involved in iron acquisition by Pseudomonas mendocina ymp
Article Snippet: Paragraph title: General DNA manipulations and sequence analysis ... The pUC19 and pGEM-T vectors (Promega) were used for cloning DNA fragments.

Article Title: The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
Article Snippet: Plasmids pPK4071 and pPK4083 were made by digesting λ430 ( ) with Nru I and cloning this 5-kb fragment containing iscRSUAhscB ( ) into the Sma I site of pUC19 ( ) and pAlter-1 (Promega), respectively. .. Plasmids pPK4196 and pPK4194 were generated by cloning PCR products containing only the ORFs for iscS and iscSUA into pET11a , respectively (Fig. ).

Nested PCR:

Article Title: Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis
Article Snippet: The 5′ end of the gfp transcript, which is initiated by P3, was then amplified with random-primer reverse transcription and nested PCR using gfp specific primers with the provided primers in the kit. .. PCR products were then inserted into pUC19 (Promega, Madison, WI) for DNA sequencing.

Rapid Amplification of cDNA Ends:

Article Title: Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis
Article Snippet: Paragraph title: 5′ Rapid amplification of cDNA ends (RACE) ... PCR products were then inserted into pUC19 (Promega, Madison, WI) for DNA sequencing.

Plasmid Preparation:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The resulting Bam HI fragments with sizes from 1.5 kb to 7.5 kb were recovered from agarose gel using the QIAquick Gel Extraction Kit (Qiagen). .. The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water. .. The ligations were performed at 4 °C for 12 h, and the ligation products were desalted using MFTM membrane filters (0.025 μm, VSWP, Millipore) before being electroporated into E. coli strain Trans5α.

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: Paragraph title: 3.3 Sequence-specific nuclear import of plasmid DNA ... In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Article Title: Inhibition of Hepatitis B Virus Replication by MyD88 Involves Accelerated Degradation of Pregenomic RNA and Nuclear Retention of Pre-S/S RNAs
Article Snippet: Taken together, our results define a novel antiviral mechanism against HBV mediated by MyD88. .. To construct HBV plasmid pHBV1.3, a terminally redundant (1.3× copy), replication-competent HBV genome (subtype adw; nucleotides 957 to 1952; GenBank accession number ) was inserted into pUC19 (Promega). pCIdA-HBV expresses all the HBV proteins, but it lacks the 5′ ɛ RNA signal, which eliminates pregenomic RNA packaging and DNA replication ( ). .. Both pCMV-HBV ( ) and pTet-HBV ( ) contain the wild-type HBV 1.1-mer overlength genomic sequence.

Article Title: Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins
Article Snippet: An E. coli -LAB shuttle vector, pLES003-b, which carries the AatII fragment reversed from pLES003 (DDBJ accession no. ) ( ) as the replication origin, was used for the promoter assay and the reconstruction of the brevicin 174A biosynthetic gene cluster. .. E. coli DH5α and the pUC19, pGEM-T (Promega), and pTA2 (Toyobo) plasmids were used for DNA cloning and sequencing.

Article Title: Identification, isolation, and analysis of a gene cluster involved in iron acquisition by Pseudomonas mendocina ymp
Article Snippet: Cosmid and plasmid DNA were manipulated and purified by standard techniques ( ). .. The pUC19 and pGEM-T vectors (Promega) were used for cloning DNA fragments.

Article Title: The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli
Article Snippet: Paragraph title: Plasmid Construction. ... Plasmids pPK4071 and pPK4083 were made by digesting λ430 ( ) with Nru I and cloning this 5-kb fragment containing iscRSUAhscB ( ) into the Sma I site of pUC19 ( ) and pAlter-1 (Promega), respectively.

Lambda Miniprep:

Article Title: Host Ranges of Listeria-Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
Article Snippet: Phage 20422-1 DNA was purified from phage isolated following infections of L. monocytogenes DP-L862 using the Lambda miniprep phage extraction kit (Qiagen, Valencia, CA). .. The DNA was digested with NheI (New England Biolabs, Waverly, MA) and cloned into pUC19 (Promega, Madison, WI) digested with XbaI (New England Biolabs).

Positron Emission Tomography:

Article Title: Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins
Article Snippet: E. coli BL21(DE3) and the pET-28a(+) plasmid (Novagen) were used for the overexpression of BreD and BreG gene products. .. E. coli DH5α and the pUC19, pGEM-T (Promega), and pTA2 (Toyobo) plasmids were used for DNA cloning and sequencing.

Agarose Gel Electrophoresis:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The resulting Bam HI fragments with sizes from 1.5 kb to 7.5 kb were recovered from agarose gel using the QIAquick Gel Extraction Kit (Qiagen). .. The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water.

In Situ:

Article Title: Intracellular trafficking of nucleic acids
Article Snippet: When protein-free, purified SV40 DNA (5243 bp) was microinjected into the cytoplasm of TC7 African Green monkey kidney cells, the majority of plasmids were detected in cell nuclei by 6 – 8 h after injection by in situ hybridisation. .. In these non-dividing cells, whereas SV40 DNA localised in the nuclei with 8 h, other plasmids, including pBR322, pUC19 and pGL3-basic (Promega Corp.), did not enter the nuclei until the cells were allowed to divide [ , ].

Protein Binding:

Article Title: Inhibition of Hepatitis B Virus Replication by MyD88 Involves Accelerated Degradation of Pregenomic RNA and Nuclear Retention of Pre-S/S RNAs
Article Snippet: To construct HBV plasmid pHBV1.3, a terminally redundant (1.3× copy), replication-competent HBV genome (subtype adw; nucleotides 957 to 1952; GenBank accession number ) was inserted into pUC19 (Promega). pCIdA-HBV expresses all the HBV proteins, but it lacks the 5′ ɛ RNA signal, which eliminates pregenomic RNA packaging and DNA replication ( ). .. Both pCMV-HBV ( ) and pTet-HBV ( ) contain the wild-type HBV 1.1-mer overlength genomic sequence.

DNA Purification:

Article Title: Identification, isolation, and analysis of a gene cluster involved in iron acquisition by Pseudomonas mendocina ymp
Article Snippet: Genomic DNA from P. mendocina ymp was prepared according to Promega’s Wizard Genomic DNA Purification Kit. .. The pUC19 and pGEM-T vectors (Promega) were used for cloning DNA fragments.

Gel Extraction:

Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes
Article Snippet: The resulting Bam HI fragments with sizes from 1.5 kb to 7.5 kb were recovered from agarose gel using the QIAquick Gel Extraction Kit (Qiagen). .. The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water.

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    Promega plasmid puc19
    Schematic representation of the lipoplexes multilamellar structure with the fluorescent probes within the DNA and the lipid. ( A ) Acceptor ( a ) on the DNA (EtBr) and donor ( d ) on the lipid (DPH-PC and BODIPY-PC). This arrangement was used for <t>DOTAP/pUC19</t> charge ratio (+/−) = 0.5. ( B ) Acceptor on the lipid (BODIPY-PC) and donor on the DNA (BOBO-1). This arrangement was used for DOTAP/pUC19 charge ratio (+/−) = 0.5, 2, and 4.
    Plasmid Puc19, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Schematic representation of the lipoplexes multilamellar structure with the fluorescent probes within the DNA and the lipid. ( A ) Acceptor ( a ) on the DNA (EtBr) and donor ( d ) on the lipid (DPH-PC and BODIPY-PC). This arrangement was used for DOTAP/pUC19 charge ratio (+/−) = 0.5. ( B ) Acceptor on the lipid (BODIPY-PC) and donor on the DNA (BOBO-1). This arrangement was used for DOTAP/pUC19 charge ratio (+/−) = 0.5, 2, and 4.

    Journal:

    Article Title: Characterization of DNA/Lipid Complexes by Fluorescence Resonance Energy Transfer

    doi:

    Figure Lengend Snippet: Schematic representation of the lipoplexes multilamellar structure with the fluorescent probes within the DNA and the lipid. ( A ) Acceptor ( a ) on the DNA (EtBr) and donor ( d ) on the lipid (DPH-PC and BODIPY-PC). This arrangement was used for DOTAP/pUC19 charge ratio (+/−) = 0.5. ( B ) Acceptor on the lipid (BODIPY-PC) and donor on the DNA (BOBO-1). This arrangement was used for DOTAP/pUC19 charge ratio (+/−) = 0.5, 2, and 4.

    Article Snippet: The plasmid pUC19 (2690 bp) was purchased from Promega (Madison, WI).

    Techniques:

    Steady-state fluorescence intensity ( A ) and fluorescence anisotropy ( B ) of BOBO-1/pUC19 complexes (30 mM Tris/HCl, pH 7.4) with different dye/base ratios, at several incubation times: 10 min (⋄), 30 min (○); 60 min (*); and 90 min (•). [DNA] = 0.006 μ M.

    Journal:

    Article Title: Characterization of DNA/Lipid Complexes by Fluorescence Resonance Energy Transfer

    doi:

    Figure Lengend Snippet: Steady-state fluorescence intensity ( A ) and fluorescence anisotropy ( B ) of BOBO-1/pUC19 complexes (30 mM Tris/HCl, pH 7.4) with different dye/base ratios, at several incubation times: 10 min (⋄), 30 min (○); 60 min (*); and 90 min (•). [DNA] = 0.006 μ M.

    Article Snippet: The plasmid pUC19 (2690 bp) was purchased from Promega (Madison, WI).

    Techniques: Fluorescence, Incubation

    Electrophoretic profile of BOBO-1/pUC19 complexes (30 mM Tris/HCl, pH 7.4) at dye:DNA base ( d / b ) values: 0 ( lane 1 ), 0.2 ( lane 2 ), 0.167 ( lane 3), 0.09 ( lane 4 ), 0.06 ( lane 5 ), 0.03 ( lane 6 ), and 0.01 ( lane 7 ), after 30 min incubation at room temperature. [DNA] = 0.03 μ M.

    Journal:

    Article Title: Characterization of DNA/Lipid Complexes by Fluorescence Resonance Energy Transfer

    doi:

    Figure Lengend Snippet: Electrophoretic profile of BOBO-1/pUC19 complexes (30 mM Tris/HCl, pH 7.4) at dye:DNA base ( d / b ) values: 0 ( lane 1 ), 0.2 ( lane 2 ), 0.167 ( lane 3), 0.09 ( lane 4 ), 0.06 ( lane 5 ), 0.03 ( lane 6 ), and 0.01 ( lane 7 ), after 30 min incubation at room temperature. [DNA] = 0.03 μ M.

    Article Snippet: The plasmid pUC19 (2690 bp) was purchased from Promega (Madison, WI).

    Techniques: Incubation

    Schematic diagrams of the HSV and recombinant viral genomes (A) Genome of wild-type HSV. Shaded boxes represent repeated sequences, and lines represent unique sequences. (B) Genome of the dl 5-29-41L triple mutant virus. The UL 5 and UL 29 genes contain deletions, and the UL 41 gene contains an insertion of a lac Z expression cassette. (C) An expanded view of the UL 41 gene disrupted by the lac Z expression cassette in dl 5-29-41L. (D) The HSV-1 KOS DNA fragment containing the UL 41 gene and flanking regions introduced into plasmid pUC19 to generate the pUC41.1 plasmid. Dashed lines define the boundaries of the homologous sequences in which recombination may have occurred to generate dl 5-29-41.1. Nucleotide numbers in (C) correspond to the HSV-2 HG-52 viral genome and in (D) to the HSV-1 strain 17 viral genome.

    Journal:

    Article Title: Construction and Properties of a Herpes Simplex Virus 2 dl5-29 Vaccine Candidate Strain Encoding an HSV-1 Virion Host Shutoff Protein

    doi: 10.1016/j.vaccine.2010.01.030

    Figure Lengend Snippet: Schematic diagrams of the HSV and recombinant viral genomes (A) Genome of wild-type HSV. Shaded boxes represent repeated sequences, and lines represent unique sequences. (B) Genome of the dl 5-29-41L triple mutant virus. The UL 5 and UL 29 genes contain deletions, and the UL 41 gene contains an insertion of a lac Z expression cassette. (C) An expanded view of the UL 41 gene disrupted by the lac Z expression cassette in dl 5-29-41L. (D) The HSV-1 KOS DNA fragment containing the UL 41 gene and flanking regions introduced into plasmid pUC19 to generate the pUC41.1 plasmid. Dashed lines define the boundaries of the homologous sequences in which recombination may have occurred to generate dl 5-29-41.1. Nucleotide numbers in (C) correspond to the HSV-2 HG-52 viral genome and in (D) to the HSV-1 strain 17 viral genome.

    Article Snippet: A 3585 bp HindIII-HpaI DNA fragment containing the HSV-1 UL 41 gene and flanking sequences was isolated from the pSG124 plasmid [ ] and cloned into the Hind III-Sma I sites of plasmid pUC19 (Promega) to generate the pUC41.1 plasmid.

    Techniques: Recombinant, Mutagenesis, Expressing, Plasmid Preparation

    Msc I partial digestion of talC-Bam HI fragment cloned in pUC19. 2 μg plasmid DNA was digested with 5 u Msc I enzyme. The digestion times are given in minutes above the gel. M, 100 bp DNA ladder makers.

    Journal: Scientific Reports

    Article Title: Rapid and efficient genome-wide characterization of Xanthomonas TAL effector genes

    doi: 10.1038/srep13162

    Figure Lengend Snippet: Msc I partial digestion of talC-Bam HI fragment cloned in pUC19. 2 μg plasmid DNA was digested with 5 u Msc I enzyme. The digestion times are given in minutes above the gel. M, 100 bp DNA ladder makers.

    Article Snippet: The obtained Bam HI fragments were ligated into Bam HI-cleaved pUC19 vector as follow: 60 ng of purified DNA fragments, 100 ng of pUC19, 2 μl of ligation buffer, and 5 u of T4 ligase (Promega) were mixed; the final reaction volume was adjusted to 20 μl with water.

    Techniques: Clone Assay, Plasmid Preparation

    Plasmid fragment generated by restriction e ndonuclease-1. Control; 2. HindIII λ DNA; 3. PUC19 vector bacteria; 4. bacteria from gut; 5. bacteria from subeschar tissue.

    Journal:

    Article Title: Tracing method study of bacterial translocation in vivo

    doi: 10.3748/wjg.v6.i1.153

    Figure Lengend Snippet: Plasmid fragment generated by restriction e ndonuclease-1. Control; 2. HindIII λ DNA; 3. PUC19 vector bacteria; 4. bacteria from gut; 5. bacteria from subeschar tissue.

    Article Snippet: PUC19 plasmid vectors (Promega): amplification of plasmid was in LB culture medium containing 100 mg/L ampicillin.

    Techniques: Plasmid Preparation, Generated