25527 Search Results


b animalis atcc 25527 t  (ATCC)
95
ATCC b animalis atcc 25527 t
Bacterial strains used to evaluate the specificity of B. animalis -specific PCR primers by a Multiplex-PCR approach
B Animalis Atcc 25527 T, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b animalis atcc 25527 t/product/ATCC
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mouse hprt target sequence  (Addgene inc)
93
Addgene inc mouse hprt target sequence
a , Schematic of the CRISPR–Cas9-based barcoding system. DSB, double-strand break. b , Location of the target site in exon 3 of the mouse <t>Hprt</t> gene. c , Examples of barcodes; the <t>germline</t> <t>sequence</t> is shown with the sgRNA target and protospacer adjacent motif (PAM) sequences indicated at top. Nucleotide additions and deletions (dashes) are indicated in red. d , Frequencies of individual barcodes in decreasing order. e , Number of Foxn1 -expressing TECs in the thymic rudiment of E12.5 embryos (left; n = 5) and numbers of different barcodes in the thymi of mice of different ages (right): E16.5, n = 6; P0, n = 5; P12–P15, n = 11; >P16, n = 12. The dotted lines indicate the range of the numbers of progenitors previously inferred from medullary islet counts in adult mice . f , Enrichment of shared barcodes in the Ly51 – UEA-1 + mTEC and Ly51 + UEA-1 – cTEC fractions of mice of different ages. Enrichment values were significantly different in the comparison of mice at P0 and >3 weeks (w) ( P = 0.009, one-sided Wilcoxon test). E16.5, n = 6; P0, n = 5; ~2 weeks, n = 11; >3 weeks, n = 11. For e and f , boxes extend from the 25th to 75th percentile; whiskers extend to the largest and smallest values; and the median is indicated. See the for a definition of the enrichment value E m . g , Co-occurrence probability of rare barcodes across pairs of samples highlighting enhanced co-occurrence in mTEC (m) and cTEC (c) fractions of the same mouse; individual mice are identified by number. Data are shown for n = 18 mice. h – k , P values (–log 10 ) of barcode frequencies indicating co-occurrence of individual barcodes in progenitor and mature TEC fractions (as defined in Fig. ) at different time points. For g – k , P values were calculated as described in the and corrected for multiple testing by the Benjamini–Hochberg method. The red numbers refer to clones discussed in the text.
Mouse Hprt Target Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse hprt target sequence/product/Addgene inc
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse hprt target sequence - by Bioz Stars, 2024-10
93/100 stars
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Image Search Results


Bacterial strains used to evaluate the specificity of B. animalis -specific PCR primers by a Multiplex-PCR approach

Journal:

Article Title: Rapid Identification, Differentiation, and Proposed New Taxonomic Classification of Bifidobacterium lactis

doi: 10.1128/AEM.68.12.6429-6434.2002

Figure Lengend Snippet: Bacterial strains used to evaluate the specificity of B. animalis -specific PCR primers by a Multiplex-PCR approach

Article Snippet: Analysis of the 16S-23S internally transcribed spacer region of B. lactis DSM10140 T , B. animalis ATCC 25527 T , and six potential B. lactis strains suggested two distinct clusters.

Techniques: Multiplex PCR

a , Schematic of the CRISPR–Cas9-based barcoding system. DSB, double-strand break. b , Location of the target site in exon 3 of the mouse Hprt gene. c , Examples of barcodes; the germline sequence is shown with the sgRNA target and protospacer adjacent motif (PAM) sequences indicated at top. Nucleotide additions and deletions (dashes) are indicated in red. d , Frequencies of individual barcodes in decreasing order. e , Number of Foxn1 -expressing TECs in the thymic rudiment of E12.5 embryos (left; n = 5) and numbers of different barcodes in the thymi of mice of different ages (right): E16.5, n = 6; P0, n = 5; P12–P15, n = 11; >P16, n = 12. The dotted lines indicate the range of the numbers of progenitors previously inferred from medullary islet counts in adult mice . f , Enrichment of shared barcodes in the Ly51 – UEA-1 + mTEC and Ly51 + UEA-1 – cTEC fractions of mice of different ages. Enrichment values were significantly different in the comparison of mice at P0 and >3 weeks (w) ( P = 0.009, one-sided Wilcoxon test). E16.5, n = 6; P0, n = 5; ~2 weeks, n = 11; >3 weeks, n = 11. For e and f , boxes extend from the 25th to 75th percentile; whiskers extend to the largest and smallest values; and the median is indicated. See the for a definition of the enrichment value E m . g , Co-occurrence probability of rare barcodes across pairs of samples highlighting enhanced co-occurrence in mTEC (m) and cTEC (c) fractions of the same mouse; individual mice are identified by number. Data are shown for n = 18 mice. h – k , P values (–log 10 ) of barcode frequencies indicating co-occurrence of individual barcodes in progenitor and mature TEC fractions (as defined in Fig. ) at different time points. For g – k , P values were calculated as described in the and corrected for multiple testing by the Benjamini–Hochberg method. The red numbers refer to clones discussed in the text.

Journal: Nature

Article Title: Developmental dynamics of two bipotent thymic epithelial progenitor types

doi: 10.1038/s41586-022-04752-8

Figure Lengend Snippet: a , Schematic of the CRISPR–Cas9-based barcoding system. DSB, double-strand break. b , Location of the target site in exon 3 of the mouse Hprt gene. c , Examples of barcodes; the germline sequence is shown with the sgRNA target and protospacer adjacent motif (PAM) sequences indicated at top. Nucleotide additions and deletions (dashes) are indicated in red. d , Frequencies of individual barcodes in decreasing order. e , Number of Foxn1 -expressing TECs in the thymic rudiment of E12.5 embryos (left; n = 5) and numbers of different barcodes in the thymi of mice of different ages (right): E16.5, n = 6; P0, n = 5; P12–P15, n = 11; >P16, n = 12. The dotted lines indicate the range of the numbers of progenitors previously inferred from medullary islet counts in adult mice . f , Enrichment of shared barcodes in the Ly51 – UEA-1 + mTEC and Ly51 + UEA-1 – cTEC fractions of mice of different ages. Enrichment values were significantly different in the comparison of mice at P0 and >3 weeks (w) ( P = 0.009, one-sided Wilcoxon test). E16.5, n = 6; P0, n = 5; ~2 weeks, n = 11; >3 weeks, n = 11. For e and f , boxes extend from the 25th to 75th percentile; whiskers extend to the largest and smallest values; and the median is indicated. See the for a definition of the enrichment value E m . g , Co-occurrence probability of rare barcodes across pairs of samples highlighting enhanced co-occurrence in mTEC (m) and cTEC (c) fractions of the same mouse; individual mice are identified by number. Data are shown for n = 18 mice. h – k , P values (–log 10 ) of barcode frequencies indicating co-occurrence of individual barcodes in progenitor and mature TEC fractions (as defined in Fig. ) at different time points. For g – k , P values were calculated as described in the and corrected for multiple testing by the Benjamini–Hochberg method. The red numbers refer to clones discussed in the text.

Article Snippet: The hU6-sgRNA Hprt transgene was cloned as a NotI fragment into the Bluescript vector and consists of the human U6 promotor (nucleotides 1–264 in GenBank accession number JN255693 ) followed by the mouse Hprt target sequence (5′-GATGGGAGGCCATCACATTGG-3′; nucleotides 255–274 in GenBank accession number J00423 ), the sgRNA backbone (nucleotides 218–139 (reverse complement) in Addgene plasmid 42250) and a short 3′ sequence (TTTTTTGGAA); for injection into fertilized eggs, the construct was linearized with SacI.

Techniques: CRISPR, Sequencing, Expressing, Clone Assay

a , Schematic of the components of the hU6:sgRNA Hprt transgene; key features are indicated by name and are colour-coded; the bar representing the hU6 promotor sequence was truncated. b , Nucleotide sequence of the hU6:sgRNA Hprt transgene construct (colour code as in a ). c – e , Frequencies of individual barcodes in decreasing order from left to right grouped by the degree of occurrence in the cohort of mice analysed here (n = 33); colours indicate those barcodes that satisfy the criterion indicated at the top right of each plot. f , Scatter plots of barcode frequencies for mTECs and cTECs from the same mouse versus barcode frequencies in cTECs isolated from two different mice. g , Fraction of informative barcodes observed in the TEC compartment of individual mice; informative barcodes are those whose P values indicate a significant deviation ( P b,i < 0.001 for barcode b in sample i ) from the barcode frequencies expected from the background model. Since these values represent singular data points, statistical comparisons were not done. Barcode data are listed in Supplementary Tables –

Journal: Nature

Article Title: Developmental dynamics of two bipotent thymic epithelial progenitor types

doi: 10.1038/s41586-022-04752-8

Figure Lengend Snippet: a , Schematic of the components of the hU6:sgRNA Hprt transgene; key features are indicated by name and are colour-coded; the bar representing the hU6 promotor sequence was truncated. b , Nucleotide sequence of the hU6:sgRNA Hprt transgene construct (colour code as in a ). c – e , Frequencies of individual barcodes in decreasing order from left to right grouped by the degree of occurrence in the cohort of mice analysed here (n = 33); colours indicate those barcodes that satisfy the criterion indicated at the top right of each plot. f , Scatter plots of barcode frequencies for mTECs and cTECs from the same mouse versus barcode frequencies in cTECs isolated from two different mice. g , Fraction of informative barcodes observed in the TEC compartment of individual mice; informative barcodes are those whose P values indicate a significant deviation ( P b,i < 0.001 for barcode b in sample i ) from the barcode frequencies expected from the background model. Since these values represent singular data points, statistical comparisons were not done. Barcode data are listed in Supplementary Tables –

Article Snippet: The hU6-sgRNA Hprt transgene was cloned as a NotI fragment into the Bluescript vector and consists of the human U6 promotor (nucleotides 1–264 in GenBank accession number JN255693 ) followed by the mouse Hprt target sequence (5′-GATGGGAGGCCATCACATTGG-3′; nucleotides 255–274 in GenBank accession number J00423 ), the sgRNA backbone (nucleotides 218–139 (reverse complement) in Addgene plasmid 42250) and a short 3′ sequence (TTTTTTGGAA); for injection into fertilized eggs, the construct was linearized with SacI.

Techniques: Sequencing, Construct, Isolation