zymogram analyses sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page  (Bio-Rad)

Journal: Scientific Reports

Article Title: Efficient secretion of a plastic degrading enzyme from the green algae Chlamydomonas reinhardtii

doi: 10.1038/s41598-025-09100-0

Figure Lengend Snippet: PHL7 glycosilation on the secretory pathway. ( A ) Schematic representation of pJP32PHL7 vector corresponding to sample loaded in lane “PHL7” on zymogram. ( B ) Schematic representation of pJP32PHL7dg (non-glycosylated PHL7) that corresponds to sample loaded in lane “PHL7dg” on zymogram. C) SDS zymogram gel with 1% v/v Impranil DLN containing Precision Plus Protein Unstained Protein Standards, Strep -tagged recombinant (Bio-Rad Laboratories #1610363) and 10X concentrated supernatant samples from pJP32PHL7 (PHL7), pJP32PHL7dg (PHL7dg), and wild-type parental cc1690 strain (wt) (from left to right). The gel displayed 2 halos (i.e., transparent bands) in the PHL7 lane and 1 halo in the PHL7dg lane after 1 day of incubation in a 100 mM potassium phosphate buffer solution, pH 8.0 at 37 °C.

Article Snippet: C) SDS zymogram gel with 1% v/v Impranil DLN containing Precision Plus Protein Unstained Protein Standards, Strep -tagged recombinant (Bio-Rad Laboratories #1610363) and 10X concentrated supernatant samples from pJP32PHL7 (PHL7), pJP32PHL7dg (PHL7dg), and wild-type parental cc1690 strain (wt) (from left to right).

Techniques: Plasmid Preparation, Recombinant, Incubation