zfand5  (Bioss)

Journal: Frontiers in Medicine

Article Title: Enhanced understanding of cinnamaldehyde’s therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect

doi: 10.3389/fmed.2024.1448937

Figure Lengend Snippet: Sequences of primers used in qRT-PCR.

Article Snippet: The membranes were initially blocked with 5% skim milk for 2 h, followed by overnight incubation at 4°C with primary antibodies targeting BCL6 (Proteintech-21187-1-AP), SGCD (ab137101), MARCKS (Proteintech-20661-1-AP), FOSL2 (CST-59971), ELL2 (Proteintech-12727-1-AP), ZFAND5 (Bioss-bs-10142R), BCL-2 (ab238042), and β -actin (ab8226).

Techniques:

Journal: Nucleic Acids Research

Article Title: RNA–RNA interactions between respiratory syncytial virus and miR-26 and miR-27 are associated with regulation of cell cycle and antiviral immunity

doi: 10.1093/nar/gkae116

Figure Lengend Snippet: Pseudogenes and lncRNAs are HC miRNA targets and nuclear localisation of AGO2-miR-27 suggests potential roles in transcriptional gene regulation. ( A ) Enrichment of HC target sites in regulatory regions compared to non-filtered target sites. Target sites were filtered to remove sites overlapping 3′UTRs and CDS. Shown are the mean ± SD for three Mock and four RSV CLEAR-CLIP replicates combined. Red asterisks indicate significant enrichment; One Brown-Forsythe and Welch ANOVA with Dunnett's T3 multiple comparisons test. Due to low number of target sites in some of the categories, Mock and RSV samples were analysed together for more statistical power, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ‘Overlapping’ indicates regulatory regions overlapping other genome annotations (pseudogenes (PSG), lncRNAs, introns, and other). (B ) Genome browser view of the genomic location of top miR-27 regulatory target overlapping a PSG, showing the conservation using PhyloP100, features of the genomic locus, total RNA-seq coverage in Mock and RSV-infected samples ( n = 3), as well as miRNA target sites coverage in Mock and RSV-infected CLEAR-CLIP samples. ( C ) RT-qPCR results for Malat1, RPL30, ZFAND5, ZFAND5 PSG lncRNA and miR-27a (left to right) levels in cytoplasmic (cyto) and nuclear (nuc) fractions in Mock and RSV-infected cells. Data is shown as mean ± SD for two biological replicates. ( D ) Western blot analysis of AGO2 protein levels in subcellular fractions showing whole cell lysate (WCL), cytoplasmic (cyto), and nuclear (nuc) fractions for Mock and RSV-infected cells. α-Tubulin and Calreticulin were used as cytoplasmic markers and Histone H3 as nuclear marker. ( E ) Quantification of AGO2 protein levels in (D). Data is shown as mean ± SD for four biological replicates and normalised to WCL Mock. ( F ) Western blot analysis of cellular fractionation of naïve A549 showing cytoplasmic fraction as well as the soluble and insoluble nuclear fractions. α-Tubulin and Calreticulin were used as cytoplasmic markers and Histone H3 as nuclear marker associated with chromatin. ( G ) RT-qPCR results of PSG lncRNA expression after treatment with 5 nM GapmeR targeting PSG lncRNA (anti-PSG lncRNA) or control. Shown are three biological replicates with mean ± SD. Significance was tested with unpaired two-tailed t -test (* P ≤ 0.05). ( H ) Western blot analysis of CEACAM1 after treatment with anti-PSG lncRNA GapmeR, siRNAs against ZFAND5 (siZFAND5) and CEACAM1 (siCEACAM1) and controls compared to β-Actin loading control. ( I ) Quantification of CEACAM1 protein levels from (H) corrected for β-Actin and normalised to Untreated control. Shown are two biological replicates with mean ± SD. Significance was tested with One Brown-Forsythe and Welch ANOVA with Dunnett's T3 multiple comparisons test.

Article Snippet: Primary antibodies used in this study: AGO2 (1:1500, clone C34C6, Cell signalling, 2897), α-Tubulin (1:2000, Cusabio, CSB-PA004344), β-Actin (1:1000, Cell signalling, 4967), Calreticulin (1:2000, Cell signalling, 2891), CEACAM1 (1:20000, Abcam, ab235598), Histone H3 (1:2000, Cell signalling, 4499), ZFAND5 (1:1000, Sigma, HPA018129).

Techniques: RNA Sequencing Assay, Infection, Quantitative RT-PCR, Western Blot, Marker, Cell Fractionation, Expressing, Two Tailed Test

Journal: Nutrients

Article Title: Effects of an Encapsulated Fruit and Vegetable Juice Concentrate on Obesity-Induced Systemic Inflammation: A Randomised Controlled Trial

doi: 10.3390/nu9020116

Figure Lengend Snippet: Microarray analysis: biologically-relevant genes that were differentially regulated following F&V concentrate supplementation in subjects with high baseline CRP (≥3.0 mg/mL).

Article Snippet: Taqman Assay IDs are as follows; phosphomevalonate kinase (PMVK) (Hs00559915_m1) and zinc finger AN1-type containing 5 (ZFAND5) (Hs04400278_g1).

Techniques: Microarray, Activation Assay, Transformation Assay, Transduction, Translocation Assay, Activity Assay, Binding Assay, Expressing

Journal: Nutrients

Article Title: Effects of an Encapsulated Fruit and Vegetable Juice Concentrate on Obesity-Induced Systemic Inflammation: A Randomised Controlled Trial

doi: 10.3390/nu9020116

Figure Lengend Snippet: Validation of microarray analysis; fold changes in gene expression following the intervention as determined by microarray versus quantitative polymerase chain reaction (qPCR). Zinc finger, AN1-type domain 5 (ZFAND5); Phosphomevalonate kinase (PMVK).

Article Snippet: Taqman Assay IDs are as follows; phosphomevalonate kinase (PMVK) (Hs00559915_m1) and zinc finger AN1-type containing 5 (ZFAND5) (Hs04400278_g1).

Techniques: Biomarker Discovery, Microarray, Gene Expression, Real-time Polymerase Chain Reaction

Journal: Nutrients

Article Title: Effects of an Encapsulated Fruit and Vegetable Juice Concentrate on Obesity-Induced Systemic Inflammation: A Randomised Controlled Trial

doi: 10.3390/nu9020116

Figure Lengend Snippet: Change in peripheral blood mRNA expression of PMVK ( A ) and ZFAND5 ( B ) at baseline compared to Week 8 in the fruit and vegetable juice concentrate (F&V concentrate) and placebo groups; measured using qPCR; the data presented are the median (IQR); the statistical test used was the Mann–Whitney test; * p < 0.05 versus baseline.

Article Snippet: Taqman Assay IDs are as follows; phosphomevalonate kinase (PMVK) (Hs00559915_m1) and zinc finger AN1-type containing 5 (ZFAND5) (Hs04400278_g1).

Techniques: Expressing, MANN-WHITNEY