Journal: PLOS One
Article Title: Altered Bcl-2/Caspase signaling and hypoxia-induced apoptosis in primary human aniridia limbal stromal cells, in CoCl 2 mediated hypoxic stress, in vitro
doi: 10.1371/journal.pone.0328157
Figure Lengend Snippet: Data represent mean±SD from three independent experiments. Two-way ANOVA followed by Dunnett’s test was used, significant p values are indicated. p21, p27, XIAP, TNF-α, and Survivin protein levels are shown. Representative histograms show primary antibody staining in green, while staining with the corresponding secondary antibody alone (negative control) is shown in red. MFI: mean fluorescence intensity, normalized to the secondary antibody control. In AN-LSCs, p21 and p27 protein levels were significantly higher (p = 0.0003; p = 0.0164) and XIAP and Survivin protein levels were significantly lower (p = 0.0003; p = 0.0074), than in LSCs, using flow cytometry. In addition, using ELISA, in cell culture supernatant of AN-LSCs, TNF-α protein levels were significantly higher following 75 µM CoCl 2 treatment, than in untreated controls (p = 0.0251).
Article Snippet: XIAP Polyclonal antibody , XIAP , Proteintech , #23453–1-AP , Rabbit , 1:100.
Techniques: Staining, Negative Control, Fluorescence, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture