Journal: Journal of Immunology Research
Article Title: KIF26B and CREB3L1 Derived from Immunoscore Could Inhibit the Progression of Ovarian Cancer
doi: 10.1155/2024/4817924
Figure Lengend Snippet: Knockdown of KIF26B and CREB3L1 gene expression inhibited the expression of PCNA and the Wnt/ β -catenin signaling pathway. (a) The detection of the expression levels of PCNA, Wnt5 a/b, and β -catenin protein by WB when the expression of KIF26B and CREB3L1 was decreased. (b) The statistical quantification of the results of WB. The n in the figure represented the number of repeated experiments. The statistical method was one-way ANOVA followed by Dunnett's test for multiple comparisons. (ns: nonsignificant, ∗∗ P < 0.01, ∗∗∗ P < 0.001).
Article Snippet: The primary antibodies used in the present study included anti-PCNA antibody (Proteintech, #10205-2-AP, 1 : 1,000 dilution), anti-Wnt5 a/b antibody (Proteintech, #55184-1-AP, 1 : 1,000 dilution), anti- β catenin antibody (Proteintech, #51067-2-AP, 1 : 1,000 dilution), and anti-GAPDH antibody (Proteintech, #10494-1-AP, 1 : 2,000 dilution).
Techniques: Knockdown, Gene Expression, Expressing