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Binding of increasing concentrations of panitumumab-[ 197 Hg]Hg-AuNPs to U251-Luc cells in the absence (total binding; TB) or presence of an excess of panitumumab (non-specific binding; NSB). Specific binding (SB) was calculated by subtracting NSB from TB. Fitting the SB curve to a one-site receptor binding model, the estimated K D = 1.8 × 10 –9 mol/L and B max = 47.44 fmole, corresponding to 1.1 × 10 5 <t>EGFR/cell</t>
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Binding of increasing concentrations of panitumumab-[ 197 Hg]Hg-AuNPs to U251-Luc cells in the absence (total binding; TB) or presence of an excess of panitumumab (non-specific binding; NSB). Specific binding (SB) was calculated by subtracting NSB from TB. Fitting the SB curve to a one-site receptor binding model, the estimated K D = 1.8 × 10 –9 mol/L and B max = 47.44 fmole, corresponding to 1.1 × 10 5 <t>EGFR/cell</t>
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Binding of increasing concentrations of panitumumab-[ 197 Hg]Hg-AuNPs to U251-Luc cells in the absence (total binding; TB) or presence of an excess of panitumumab (non-specific binding; NSB). Specific binding (SB) was calculated by subtracting NSB from TB. Fitting the SB curve to a one-site receptor binding model, the estimated K D = 1.8 × 10 –9 mol/L and B max = 47.44 fmole, corresponding to 1.1 × 10 5 EGFR/cell

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Auger electron-emitting EGFR-targeted and non-targeted [ 197 Hg]Hg-gold nanoparticles for treatment of glioblastoma multiforme (GBM)

doi: 10.1186/s41181-025-00367-2

Figure Lengend Snippet: Binding of increasing concentrations of panitumumab-[ 197 Hg]Hg-AuNPs to U251-Luc cells in the absence (total binding; TB) or presence of an excess of panitumumab (non-specific binding; NSB). Specific binding (SB) was calculated by subtracting NSB from TB. Fitting the SB curve to a one-site receptor binding model, the estimated K D = 1.8 × 10 –9 mol/L and B max = 47.44 fmole, corresponding to 1.1 × 10 5 EGFR/cell

Article Snippet: To maximize the potency of the AE emitted by 197 Hg, we further conjugated these [ 197 Hg]Hg-AuNPs to the anti-epidermal growth factor receptor (EGFR) monoclonal antibody, panitumumab (Vectibix®, Amgen) which binds and internalizes them into GBM cells.

Techniques: Binding Assay