usp10 (Proteintech)
Structured Review
Usp10, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Antibody/pmc12968915-44-42-47
Average 95 stars, based on 43 article reviews
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Blocking Assay:Article Title: ERβ/circAHNAK Axis Inhibits USP10–FMR1 Deubiquitination to Prevent m⁶A‐Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma Article Snippet: [ , ] Protein concentrations were quantified, and equal amounts were separated by SDS‐PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948‐1‐AP, Proteintech, China), FMR1(1, 2000,13755‐1‐AP, Proteintech, China), USP10( Article Title: ERβ/circAHNAK Axis Inhibits USP10-FMR1 Deubiquitination to Prevent m⁶A-Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma. Article Snippet: [64,65] Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948-1-AP, Proteintech, China), FMR1(1, 2000,13755-1-AP, Proteintech, China), USP10( Incubation:Article Title: ERβ/circAHNAK Axis Inhibits USP10–FMR1 Deubiquitination to Prevent m⁶A‐Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma Article Snippet: [ , ] Protein concentrations were quantified, and equal amounts were separated by SDS‐PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948‐1‐AP, Proteintech, China), FMR1(1, 2000,13755‐1‐AP, Proteintech, China), USP10( Article Title: Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis Article Snippet: .. For western blot analysis, After SDS-PAGE (10%) and PVDF transfer (Millipore), membranes were blocked (5% milk, 1 h RT) then incubated with primary antibodies (4°C, overnight): MRPS7 (1:1000, Abcam, UK), MRPS23 (1:1000, Proteintech, USA), β-catenin (1:1000, Proteintech, USA), Slug (1:1000, Proteintech, USA), Vimentin (1:1000, Proteintech, USA), E-cadherin (1:1000, Proteintech, USA), Article Title: ERβ/circAHNAK Axis Inhibits USP10-FMR1 Deubiquitination to Prevent m⁶A-Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma. Article Snippet: [64,65] Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948-1-AP, Proteintech, China), FMR1(1, 2000,13755-1-AP, Proteintech, China), USP10( Western Blot:Article Title: Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis Article Snippet: .. For western blot analysis, After SDS-PAGE (10%) and PVDF transfer (Millipore), membranes were blocked (5% milk, 1 h RT) then incubated with primary antibodies (4°C, overnight): MRPS7 (1:1000, Abcam, UK), MRPS23 (1:1000, Proteintech, USA), β-catenin (1:1000, Proteintech, USA), Slug (1:1000, Proteintech, USA), Vimentin (1:1000, Proteintech, USA), E-cadherin (1:1000, Proteintech, USA), Article Title: USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2 Article Snippet: cTNT , Proteintech , 15513-1-AP , WB: 1:1000. .. SDS Page:Article Title: Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis Article Snippet: .. For western blot analysis, After SDS-PAGE (10%) and PVDF transfer (Millipore), membranes were blocked (5% milk, 1 h RT) then incubated with primary antibodies (4°C, overnight): MRPS7 (1:1000, Abcam, UK), MRPS23 (1:1000, Proteintech, USA), β-catenin (1:1000, Proteintech, USA), Slug (1:1000, Proteintech, USA), Vimentin (1:1000, Proteintech, USA), E-cadherin (1:1000, Proteintech, USA), FLAG-tag:Article Title: FAD synthase confers ferroptosis resistance and restrains CD8 + T cell recruitment in hepatocellular carcinoma. Article Snippet: .. Antibody information: β-actin (CST, #4970, 1:1000), RFK (Santa Cruz, #sc-398830, 1:500), FADS (Proteintech, #68491-1-Ig, 1:5000), PCBP2 (ABclonal, #A23987, 1:2000), FLAG-tag (ABclonal, #AE005, 1:10000), His-tag (ABclonal, #AE003, 1:5000), HA-tag (ABclonal, #AE008, 1:2000), Article Title: FAD synthase confers ferroptosis resistance and restrains CD8 + T cell recruitment in hepatocellular carcinoma Article Snippet: .. Antibody information: β-actin (CST, #4970, 1:1000), RFK (Santa Cruz, #sc-398830, 1:500), FADS (Proteintech, #68491-1-Ig, 1:5000), PCBP2 (ABclonal, #A23987, 1:2000), FLAG-tag (ABclonal, #AE005, 1:10000), His-tag (ABclonal, #AE003, 1:5000), HA-tag (ABclonal, #AE008, 1:2000), other:Article Title: USP10 regulates HIF2α stability by deubiquitinating HIF2α regardless of VHL status in kidney cancer Article Snippet: The membranes were blocked with 5% milk for 1 hour at room temperature, washed three times with Tris-buffered saline with Tween 20 (TBST), and then incubated at 4 °C overnight with antibodies of interest (HIF2α, ab207607, RRID: AB_2618694, 1:1,000, Abcam, Shanghai, China; USP10, 19374-1-AP, RRID: AB_10858617, 1:1,000, Proteintech Group, Inc., Wuhan, China, and β-actin, #4967, RRID: AB_330288, 1:1,000, Cell Signaling Technology, Shanghai, China). |
