Review




Structured Review

Proteintech usp10
Usp10, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Antibody/pmc12968915-44-42-47
Average 95 stars, based on 43 article reviews
usp10 - by Bioz Stars, 2026-10
95/100 stars

Images

Related Articles

Blocking Assay:

Article Title: ERβ/circAHNAK Axis Inhibits USP10–FMR1 Deubiquitination to Prevent m⁶A‐Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma
Article Snippet: [ , ] Protein concentrations were quantified, and equal amounts were separated by SDS‐PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948‐1‐AP, Proteintech, China), FMR1(1, 2000,13755‐1‐AP, Proteintech, China), USP10(1, 1000,19374‐1‐AP, Proteintech, China), Ub(1, 10 000,80992‐1‐RR, Proteintech, China), FLAG(1, 20 000, 20543‐1‐AP, Proteintech, China), CD9(1, 30 000, 20597‐1‐AP, Proteintech, China), HSP70 (1, 10 000, 10995‐1‐AP, Proteintech, China), TSG101 (1, 8000, 28283‐1‐AP, Proteintech, China), Calnexin (1, 10 000, 10427‐2‐AP, Proteintech, China), and GAPDH (1, 50 000, 60004‐1‐IG, Proteintech, China). .. After incubation with HRP‐conjugated secondary antibodies (1, 10 000, RGAR001/RGAM001, Proteintech, China), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France).

Article Title: ERβ/circAHNAK Axis Inhibits USP10-FMR1 Deubiquitination to Prevent m⁶A-Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma.
Article Snippet: [64,65] Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948-1-AP, Proteintech, China), FMR1(1, 2000,13755-1-AP, Proteintech, China), USP10(1, 1000,19374-1-AP, Proteintech, China), Ub(1, 10 000,80992-1-RR, Proteintech, China), FLAG(1, 20 000, 20543-1-AP, Proteintech, China), CD9(1, 30 000, 20597-1-AP, Proteintech, China), HSP70 (1, 10 000, 10995-1-AP, Proteintech, China), TSG101 (1, 8000, 28283-1-AP, Proteintech, China), Calnexin (1, 10 000, 10427-2-AP, Proteintech, China), and GAPDH (1, 50 000, 60004-1-IG, Proteintech, China). .. After incubation with HRPconjugated secondary antibodies (1, 10 000, RGAR001/RGAM001, Proteintech, China), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France).

Incubation:

Article Title: ERβ/circAHNAK Axis Inhibits USP10–FMR1 Deubiquitination to Prevent m⁶A‐Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma
Article Snippet: [ , ] Protein concentrations were quantified, and equal amounts were separated by SDS‐PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948‐1‐AP, Proteintech, China), FMR1(1, 2000,13755‐1‐AP, Proteintech, China), USP10(1, 1000,19374‐1‐AP, Proteintech, China), Ub(1, 10 000,80992‐1‐RR, Proteintech, China), FLAG(1, 20 000, 20543‐1‐AP, Proteintech, China), CD9(1, 30 000, 20597‐1‐AP, Proteintech, China), HSP70 (1, 10 000, 10995‐1‐AP, Proteintech, China), TSG101 (1, 8000, 28283‐1‐AP, Proteintech, China), Calnexin (1, 10 000, 10427‐2‐AP, Proteintech, China), and GAPDH (1, 50 000, 60004‐1‐IG, Proteintech, China). .. After incubation with HRP‐conjugated secondary antibodies (1, 10 000, RGAR001/RGAM001, Proteintech, China), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France).

Article Title: Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis
Article Snippet: .. For western blot analysis, After SDS-PAGE (10%) and PVDF transfer (Millipore), membranes were blocked (5% milk, 1 h RT) then incubated with primary antibodies (4°C, overnight): MRPS7 (1:1000, Abcam, UK), MRPS23 (1:1000, Proteintech, USA), β-catenin (1:1000, Proteintech, USA), Slug (1:1000, Proteintech, USA), Vimentin (1:1000, Proteintech, USA), E-cadherin (1:1000, Proteintech, USA), USP10 (1:1000, Proteintech, USA), SOX2 (1:1000, Proteintech, USA), Oct4 (1:1000, Abcam, UK), and Nanog (1:1000, Abcam, UK). .. After washing with PBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; Proteintech, USA) for 60 minutes at ambient temperature.

Article Title: ERβ/circAHNAK Axis Inhibits USP10-FMR1 Deubiquitination to Prevent m⁶A-Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma.
Article Snippet: [64,65] Protein concentrations were quantified, and equal amounts were separated by SDS-PAGE before transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with 5% skim milk for 2 h, membranes were incubated with primary antibodies, ERβ (1, 2000, GTX70174, GeneTex, USA), ADAM17 (1, 8000,29948-1-AP, Proteintech, China), FMR1(1, 2000,13755-1-AP, Proteintech, China), USP10(1, 1000,19374-1-AP, Proteintech, China), Ub(1, 10 000,80992-1-RR, Proteintech, China), FLAG(1, 20 000, 20543-1-AP, Proteintech, China), CD9(1, 30 000, 20597-1-AP, Proteintech, China), HSP70 (1, 10 000, 10995-1-AP, Proteintech, China), TSG101 (1, 8000, 28283-1-AP, Proteintech, China), Calnexin (1, 10 000, 10427-2-AP, Proteintech, China), and GAPDH (1, 50 000, 60004-1-IG, Proteintech, China). .. After incubation with HRPconjugated secondary antibodies (1, 10 000, RGAR001/RGAM001, Proteintech, China), protein bands were visualized using chemiluminescent HRP substrate (Millipore, USA) and detected with the Fusion FX imaging system (Vilber Lourmat, France).

Western Blot:

Article Title: Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis
Article Snippet: .. For western blot analysis, After SDS-PAGE (10%) and PVDF transfer (Millipore), membranes were blocked (5% milk, 1 h RT) then incubated with primary antibodies (4°C, overnight): MRPS7 (1:1000, Abcam, UK), MRPS23 (1:1000, Proteintech, USA), β-catenin (1:1000, Proteintech, USA), Slug (1:1000, Proteintech, USA), Vimentin (1:1000, Proteintech, USA), E-cadherin (1:1000, Proteintech, USA), USP10 (1:1000, Proteintech, USA), SOX2 (1:1000, Proteintech, USA), Oct4 (1:1000, Abcam, UK), and Nanog (1:1000, Abcam, UK). .. After washing with PBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; Proteintech, USA) for 60 minutes at ambient temperature.

Article Title: USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2
Article Snippet: cTNT , Proteintech , 15513-1-AP , WB: 1:1000. .. USP10 , Proteintech , 19374-1-AP , WB: 1:1000 IP: 1:100. .. Yy1 , Proteintech , 66281-1-Ig , WB: 1:1000 CHIP: 1:50.

SDS Page:

Article Title: Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis
Article Snippet: .. For western blot analysis, After SDS-PAGE (10%) and PVDF transfer (Millipore), membranes were blocked (5% milk, 1 h RT) then incubated with primary antibodies (4°C, overnight): MRPS7 (1:1000, Abcam, UK), MRPS23 (1:1000, Proteintech, USA), β-catenin (1:1000, Proteintech, USA), Slug (1:1000, Proteintech, USA), Vimentin (1:1000, Proteintech, USA), E-cadherin (1:1000, Proteintech, USA), USP10 (1:1000, Proteintech, USA), SOX2 (1:1000, Proteintech, USA), Oct4 (1:1000, Abcam, UK), and Nanog (1:1000, Abcam, UK). .. After washing with PBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; Proteintech, USA) for 60 minutes at ambient temperature.

FLAG-tag:

Article Title: FAD synthase confers ferroptosis resistance and restrains CD8 + T cell recruitment in hepatocellular carcinoma.
Article Snippet: .. Antibody information: β-actin (CST, #4970, 1:1000), RFK (Santa Cruz, #sc-398830, 1:500), FADS (Proteintech, #68491-1-Ig, 1:5000), PCBP2 (ABclonal, #A23987, 1:2000), FLAG-tag (ABclonal, #AE005, 1:10000), His-tag (ABclonal, #AE003, 1:5000), HA-tag (ABclonal, #AE008, 1:2000), USP10 (Proteintech, #19374-1-AP, 1:1000), cGAS (Proteintech, #26416- 1-AP, 1:2000), STING (Proteintech, #80365-RR, 1:1000), TBK1 (CST, #3504, 1:1000), phospho-TBK1 (CST, #5483, 1:1000), IRF3 (Proteintech, #11312-1, 1:5000), and phospho-IRF3 (CST, #29047, 1:1000). ..

Article Title: FAD synthase confers ferroptosis resistance and restrains CD8 + T cell recruitment in hepatocellular carcinoma
Article Snippet: .. Antibody information: β-actin (CST, #4970, 1:1000), RFK (Santa Cruz, #sc-398830, 1:500), FADS (Proteintech, #68491-1-Ig, 1:5000), PCBP2 (ABclonal, #A23987, 1:2000), FLAG-tag (ABclonal, #AE005, 1:10000), His-tag (ABclonal, #AE003, 1:5000), HA-tag (ABclonal, #AE008, 1:2000), USP10 (Proteintech, #19374-1-AP, 1:1000), cGAS (Proteintech, #26416-1-AP, 1:2000), STING (Proteintech, #80365-RR, 1:1000), TBK1 (CST, #3504, 1:1000), phospho-TBK1 (CST, #5483, 1:1000), IRF3 (Proteintech, #11312-1, 1:5000), and phospho-IRF3 (CST, #29047, 1:1000). ..

other:

Article Title: USP10 regulates HIF2α stability by deubiquitinating HIF2α regardless of VHL status in kidney cancer
Article Snippet: The membranes were blocked with 5% milk for 1 hour at room temperature, washed three times with Tris-buffered saline with Tween 20 (TBST), and then incubated at 4 °C overnight with antibodies of interest (HIF2α, ab207607, RRID: AB_2618694, 1:1,000, Abcam, Shanghai, China; USP10, 19374-1-AP, RRID: AB_10858617, 1:1,000, Proteintech Group, Inc., Wuhan, China, and β-actin, #4967, RRID: AB_330288, 1:1,000, Cell Signaling Technology, Shanghai, China).



Similar Products

94
Cyagen Biosciences usp10 mice
Usp10 Mice, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/Usp10/pm41927540-271-0-5
Average 94 stars, based on 1 article reviews
usp10 mice - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

94
Cyagen Biosciences usp10fl
Usp10fl, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/Usp10/pm41927540-1292-0-5
Average 94 stars, based on 1 article reviews
usp10fl - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

95
Proteintech usp10
Usp10, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Antibody/pmc12968915-44-42-47
Average 95 stars, based on 1 article reviews
usp10 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

95
Cell Signaling Technology Inc e cadherin
E Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Rabbit+mAb/pm41618382-134-14-16
Average 95 stars, based on 1 article reviews
e cadherin - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

94
Cell Signaling Technology Inc usp10
Usp10, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Antibody/pm41559416-182-25-26
Average 94 stars, based on 1 article reviews
usp10 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

93
Bethyl rabbit polyclonal usp10 antibody
Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, <t>USP10,</t> and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.
Rabbit Polyclonal Usp10 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Antibody/pmc12828549-18-0-5
Average 93 stars, based on 1 article reviews
rabbit polyclonal usp10 antibody - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

93
Bethyl a300 900a rrid ab 625312
Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, <t>USP10,</t> and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.
A300 900a Rrid Ab 625312, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/USP10+Antibody/pmc12828549-18-9-5
Average 93 stars, based on 1 article reviews
a300 900a rrid ab 625312 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

94
Addgene inc pfrt to flagha usp10
Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, <t>USP10,</t> and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.
Pfrt To Flagha Usp10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp10/pFRT_TO_FlagHA_USP10+(Plasmid+%23127102)/pmc12803508-358-51-52
Average 94 stars, based on 1 article reviews
pfrt to flagha usp10 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, USP10, and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.

Journal: iScience

Article Title: Chronic stress antagonizes formation of stress granules

doi: 10.1016/j.isci.2025.114556

Figure Lengend Snippet: Chronic stress-pre-incubated cells fail to disassemble polysomes by acute stress U2OS cells were subjected to treatment with 100 μM SA for 1 h after pre-incubation with 10 μM SA, 1 μM Tg (thapsigargin), 60 μM CCCP (carbonyl cyanide m -chlorophenyl hydrazone), or HBSS for 24 h. Unstressed cells (NT) were used as a control. (A) Cells were pulsed with puromycin and emetine for 5 min and lysed. Cell lysates were subjected to western blotting using antibodies for puromycin, p -eIF2α, total eIF2α, G3BP1, G3BP2, caprin1, USP10, and β-actin. Three blots were taken for each of the three biological replicates (independent experiments). The same colors of the circle dot in the graph are from the same independent experiment. Results are mean ± S.E.M. ( n = 3). p values were assessed using a one-way ANOVA ( p ∗ <0.05, p ∗∗ <0.01, p ∗∗∗ <0.001, p ∗∗∗∗ <0.0001). (B–F) Polysome profiles from U2OS cells. NT, black; 1 h SA, red; (B) 24 h pre-incubation of SA, blue; 1 h SA with 24 h pre-incubation of SA, green. (C) 24 h pre-incubation of Tg, blue; 1 h SA with 24 h pre-incubation of Tg, green. (D) 24 h pre-incubation of CCCP, blue; 1 h SA with 24 h pre-incubation of CCCP; green. (E) 24 h pre-incubation of HBSS, blue; 1 h SA with 24 h pre-incubation of HBSS; green. (F) The polysome/monosome ratio was calculated with normalization by each of NT as a control.

Article Snippet: Rabbit Polyclonal USP10 antibody , Bethyl Laboratories Inc. , A300-900A; RRID: AB_625312.

Techniques: Incubation, Control, Western Blot