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Proteintech upa

Multi-omics integration <t>identifies</t> <t>LAMC2,</t> <t>uPA</t> and BSSP-4 as CCA-specific secreted factors. (A) Schematic of the experimental workflow to collect material for secretome and proteome analysis. (B) Venn diagram showing the overlap of PK-induced transcriptional and secretory responses in chol-orgs. Intersections include PK-upregulated genes in chol-orgs from RNA-seq (chol-PK vs chol-WT, bottom-left), PK-upregulated proteins in chol-orgs from secretome (CCM, bottom-right), genes whose PK-induced expression is stronger in interaction effects (interaction contrast, upper-right), and proteins predicted to be secreted according to the Human Protein Atlas (upper-left). (C) Bubble plot showing cross-dataset support for candidate PK-associated genes identified by the integrative analysis in (B). Rows indicate 15 selected genes from (B), and columns indicate the indicated in-house murine result and public human datasets. Dot color encodes z-scored log2 FC (z(LFC). Filled circles indicate significant differences (padj < 0.1) and open circles indicate non-significant results. Dot size reflects statistical strength-log10(padj).

Upa, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/upa/LAMC2+Antibody/bio_rxiv__64898__2026__03__24__711280-420-10-11
Average 94 stars, based on 19 article reviews

upa - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Cell-of-Origin, not Oncogenic Effect, Determines Desmoplastic Immune Exclusion in KRAS-Driven Liver Cancer"

Article Title: Cell-of-Origin, not Oncogenic Effect, Determines Desmoplastic Immune Exclusion in KRAS-Driven Liver Cancer

Journal: bioRxiv

doi: 10.64898/2026.03.24.711280

Multi-omics integration identifies LAMC2, uPA and BSSP-4 as CCA-specific secreted factors. (A) Schematic of the experimental workflow to collect material for secretome and proteome analysis. (B) Venn diagram showing the overlap of PK-induced transcriptional and secretory responses in chol-orgs. Intersections include PK-upregulated genes in chol-orgs from RNA-seq (chol-PK vs chol-WT, bottom-left), PK-upregulated proteins in chol-orgs from secretome (CCM, bottom-right), genes whose PK-induced expression is stronger in interaction effects (interaction contrast, upper-right), and proteins predicted to be secreted according to the Human Protein Atlas (upper-left). (C) Bubble plot showing cross-dataset support for candidate PK-associated genes identified by the integrative analysis in (B). Rows indicate 15 selected genes from (B), and columns indicate the indicated in-house murine result and public human datasets. Dot color encodes z-scored log2 FC (z(LFC). Filled circles indicate significant differences (padj < 0.1) and open circles indicate non-significant results. Dot size reflects statistical strength-log10(padj).

Figure Legend Snippet: Multi-omics integration identifies LAMC2, uPA and BSSP-4 as CCA-specific secreted factors. (A) Schematic of the experimental workflow to collect material for secretome and proteome analysis. (B) Venn diagram showing the overlap of PK-induced transcriptional and secretory responses in chol-orgs. Intersections include PK-upregulated genes in chol-orgs from RNA-seq (chol-PK vs chol-WT, bottom-left), PK-upregulated proteins in chol-orgs from secretome (CCM, bottom-right), genes whose PK-induced expression is stronger in interaction effects (interaction contrast, upper-right), and proteins predicted to be secreted according to the Human Protein Atlas (upper-left). (C) Bubble plot showing cross-dataset support for candidate PK-associated genes identified by the integrative analysis in (B). Rows indicate 15 selected genes from (B), and columns indicate the indicated in-house murine result and public human datasets. Dot color encodes z-scored log2 FC (z(LFC). Filled circles indicate significant differences (padj < 0.1) and open circles indicate non-significant results. Dot size reflects statistical strength-log10(padj).

Techniques Used: Biomarker Discovery, RNA Sequencing, Expressing

LAMC2 and uPA affect HSC activation and impair CCA formation in vivo . (A) Schematic overview of the in vitro fibroblast activation assay, where murine HSCs were treated with CCM from chol-PK and neutralizing antibodies. (B) Scatter plot indicating mRNA expression of Acta2 and Col1a1 in mHSCs treated with Chol-PK CCM and indicated neutralizing antibodies or control IgG. (n=5 from 2 independent experiments). (C) Schematic of scratch assay with mHSCs treated with low FBS (2%) media supplemented with respective recombinant protein. (D) Plot depicting mean relative wound coverage upon scratch injury over time with indicated recombinant protein at different dosage (n≥17 from 3 experiments). (E) Schematic of in vivo validation model: Chol-PK organoids were edited by CRISPR/Cas9 to generate Prss22 -, Lamc2 -, or Plau -deleted lines, which were orthotopically implanted into the liver and harvested at the same time point. (F) Macroscopic images of liver tumors 7 weeks after implantation of indicated lines. (G) Bar plot with respective tumor penetrance per line. (H) Representative histopathology images of liver tumors obtained upon orthotopic implantation of chol-PK and indicated KO lines. Stains as indicated. Red dotted line demarcates the boundary between non-tumor liver (N) and tumor (T). All scale bars are 100 μm. (I) Scatter plot depicting the total CD3 + and CD8 + T cell density per area per tumor for the indicated lines.

Figure Legend Snippet: LAMC2 and uPA affect HSC activation and impair CCA formation in vivo . (A) Schematic overview of the in vitro fibroblast activation assay, where murine HSCs were treated with CCM from chol-PK and neutralizing antibodies. (B) Scatter plot indicating mRNA expression of Acta2 and Col1a1 in mHSCs treated with Chol-PK CCM and indicated neutralizing antibodies or control IgG. (n=5 from 2 independent experiments). (C) Schematic of scratch assay with mHSCs treated with low FBS (2%) media supplemented with respective recombinant protein. (D) Plot depicting mean relative wound coverage upon scratch injury over time with indicated recombinant protein at different dosage (n≥17 from 3 experiments). (E) Schematic of in vivo validation model: Chol-PK organoids were edited by CRISPR/Cas9 to generate Prss22 -, Lamc2 -, or Plau -deleted lines, which were orthotopically implanted into the liver and harvested at the same time point. (F) Macroscopic images of liver tumors 7 weeks after implantation of indicated lines. (G) Bar plot with respective tumor penetrance per line. (H) Representative histopathology images of liver tumors obtained upon orthotopic implantation of chol-PK and indicated KO lines. Stains as indicated. Red dotted line demarcates the boundary between non-tumor liver (N) and tumor (T). All scale bars are 100 μm. (I) Scatter plot depicting the total CD3 + and CD8 + T cell density per area per tumor for the indicated lines.

Techniques Used: Activation Assay, In Vivo, In Vitro, Expressing, Control, Wound Healing Assay, Recombinant, Biomarker Discovery, CRISPR, Histopathology

Membrane:

Article Title: Impact of Escherichia coli and Lipopolysaccharide on the MAPK Signaling Pathway, MMPs, TIMPs, and the uPA System in Bovine Mammary Epithelial Cells
Article Snippet: .. Then, the membrane was blocked with 5% bovine serum albumin (BSA) at room temperature for 4 h. Primary antibody incubation was carried out for 12 h at 4 °C with the following antibodies: mouse-derived anti-β-actin (Abcam, Cambridge, UK; diluted 1:10,000), rabbit-derived anti-MMP-1 (Bioworld Technology, Nanjing, China; diluted 1:300), and rabbit-derived anti-MMP-1, -2, -3, -9, -13, TIMP-1, -2, uPA, uPAR, PAI-1, ERK1/2, P38, JNK (ProteinTech Group, San Diego, CA, USA; diluted 1:500). .. After washing with TBST for 5 to 10 min, the membrane was incubated with secondary antibodies (goat anti-rabbit, diluted 1:3000, and goat anti-mouse, diluted 1:4000) for 1 h. Immunoblot signals were detected with the ECL Western blot detection kit (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

Article Title: Impact of Escherichia coli and Lipopolysaccharide on the MAPK Signaling Pathway, MMPs, TIMPs, and the uPA System in Bovine Mammary Epithelial Cells
Article Snippet: .. Then, the membrane was blocked with 5% bovine serum albumin (BSA) at room temperature for 4 h. Primary antibody incubation was carried out for 12 h at 4 ◦C with the following antibodies: mouse-derived anti-βactin (Abcam, Cambridge, UK; diluted 1:10,000), rabbit-derived anti-MMP-1 (Bioworld Technology, Nanjing, China; diluted 1:300), and rabbit-derived anti-MMP-1, -2, -3, -9, -13, TIMP-1, -2, uPA, uPAR, PAI-1, ERK1/2, P38, JNK (ProteinTech Group, San Diego, CA, USA; diluted 1:500). .. After washing with TBST for 5 to 10 min, the membrane was incubated with secondary antibodies (goat anti-rabbit, diluted 1:3000, and goat anti-mouse, diluted 1:4000) for 1 h. Immunoblot signals were detected with the ECL Western blot detection kit (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

Incubation:

Article Title: Impact of Escherichia coli and Lipopolysaccharide on the MAPK Signaling Pathway, MMPs, TIMPs, and the uPA System in Bovine Mammary Epithelial Cells
Article Snippet: .. Then, the membrane was blocked with 5% bovine serum albumin (BSA) at room temperature for 4 h. Primary antibody incubation was carried out for 12 h at 4 ◦C with the following antibodies: mouse-derived anti-βactin (Abcam, Cambridge, UK; diluted 1:10,000), rabbit-derived anti-MMP-1 (Bioworld Technology, Nanjing, China; diluted 1:300), and rabbit-derived anti-MMP-1, -2, -3, -9, -13, TIMP-1, -2, uPA, uPAR, PAI-1, ERK1/2, P38, JNK (ProteinTech Group, San Diego, CA, USA; diluted 1:500). .. After washing with TBST for 5 to 10 min, the membrane was incubated with secondary antibodies (goat anti-rabbit, diluted 1:3000, and goat anti-mouse, diluted 1:4000) for 1 h. Immunoblot signals were detected with the ECL Western blot detection kit (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

Article Title: Effects of heat stress on plasminogen activator related in milk protein in bovine mammary epithelial cells and mid lactating Holstein cow milk.
Article Snippet: Heat stress (HS) is a significant factor in reducing milk yield and protein in dairy cows.. However, studies on the mechanisms underlying HS-induced reductions in milk protein are limited.. This study investigated the effects of HS on the plasminogen activator (PA) in bovine mammary alveolar cells (MAC-T) and cow milk to investigate milk protein reduction under hyperthermia.

Western Blot:

Article Title: Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI‐1‐TGF‐ β ‐myoCAF‐Dependent Mechanism
Article Snippet: An equal amount of protein from each sample was loaded onto Mini‐PROTEAN TGX Gels (Cat. 4 561 086, BIO‐RAD), following by transferring onto PVDF membranes (Cat. 1 620 184, BIO‐RAD). .. Immunoblotting was carried out with primary antibodies specific for PAI‐1 (Cat. Ab66705, Abcam), α ‐SMA (Cat. MA5‐11547, Thermo Fisher Scientific), FAP (Cat. NBP2‐89135, Novus Biologicals), PDGFR β (Cat. 13449‐1‐AP, proteintech), uPA (Cat. 10147‐1‐AP, Proteintech), tPA (Cat. PA5‐85186, Invitrogen), and β ‐actin (Cat. 612 657, BD Biosciences). ..

Article Title: Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI-1-TGF-β-myoCAF-Dependent Mechanism.
Article Snippet: An equal amount of protein from each sample was loaded onto Mini-PROTEAN TGX Gels (Cat. 4 561 086, BIO-RAD), following by transferring onto PVDF membranes (Cat. 1 620 184, BIO-RAD). .. Immunoblotting was carried out with primary antibodies specific for PAI-1 (Cat. Ab66705, Abcam), α-SMA (Cat. MA5-11547, Thermo Fisher Scientific), FAP (Cat. NBP2-89135, Novus Biologicals), PDGFRβ (Cat. 13449-1-AP, proteintech), uPA (Cat. 10147-1-AP, Proteintech), tPA (Cat. PA5-85186, Invitrogen), and β-actin (Cat. 612 657, BD Biosciences). ..

Blocking Assay:

other:

Article Title: Cell-of-Origin, not Oncogenic Effect, Determines Desmoplastic Immune Exclusion in KRAS-Driven Liver Cancer
Article Snippet: The antibodies used in this study were: Lamc2 (Proteintech, #19698-1-AP), uPA (Proteintech, #17968-1-AP), BSSP4 (ThermoFisher Scientific, #PA5-47245), and rabbit-IgG (Millipore, #pp64).

Article Title: Role of poFUT1 and O-fucosylation in placental angiogenesis†.
Article Snippet: Trophoblast cells are critical to placental angiogenesis in the first trimester of pregnancy.. Dysfunction of trophoblast leads to defective vascular remodeling and impaired angiogenesis, which is believed as the major cause of placental insufficiency and pregnancy failure.. Protein Ofucosyltransferase 1 (poFUT1) is mainly responsible for O-fucosylated glycan biosynthesis on glycoproteins, and poFUT1 deficiency causes embryonic lethality in mice.

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