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tryptose phosphate broth tpb (Difco)

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Difco tryptose phosphate broth tpb
Tryptose Phosphate Broth Tpb, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tpb/broth+phosphate+tryptose/pmc12704782-37-35-40
Average 86 stars, based on 1 article reviews

tryptose phosphate broth tpb - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Evaluation of real-time reverse-transcription loop-mediated isothermal amplification assay for clinical diagnosis of West Nile virus in patients
Article Snippet: TPB (DIFCO, USA), 10 per cent foetal bovine serum (Sigma, USA), 3 per cent L-glutamine and gentamicin (80 mg/l) (Sigma, USA) were purchased.

Cell Culture:

Article Title: Ixodes ovatus Ehrlichia Exhibits Unique Ultrastructural Characteristics inMammalian Endothelial and Tick-derived Cells
Article Snippet: .. RF/6A cells were obtained from the American Tissue Type Culture Association (ATCC, Manassas, VA, USA; ATCC CRL-1780) and were cultured in L15B300 16 supplemented with 5% FBS (heat-inactivated; Harlan, Indianapolis, IN, USA), 5% TPB (Difco, Detroit, MI, USA), 0.1% lipoprotein concentrate (MP Biomedical, Irvine, CA, USA), 25 mM HEPES, 0.25% NaHCO 3 , adjusted to pH ~7.5, in the absence of antibiotics at 37°C in a 5% CO 2 -atmosphere. ..

Incubation:

Article Title: Investigating the reassortment potential and pathogenicity of the S segment in Akabane virus using a reverse genetics system.
Article Snippet: Each well was transfected with 1 μg of pTVT7_AKAV-7_L, 1 μg of pTVT7_AKAV-7_M, 1 μg of pTVT7_AKAV-7_S, 0.5 μg of pTM1_AKAV-7_RdRp, and 0.5 μg of pTM1_AKAV-7_N using 2 μL of Lipofectamine-3000 Transfection Reagent (Invitrogen, USA) per μg of DNA in 500 μL of Opti-MEM (Gibco, USA). .. After 24 h of incubation at 37 °C, the Opti-MEM was replaced with 500 μL of MEM containing 1% antibiotic/ antimycotic solution (Gibco, USA), 3% FBS, and 10% TPB (BD, Difco, USA). ..

Article Title: Investigating the reassortment potential and pathogenicity of the S segment in Akabane virus using a reverse genetics system
Article Snippet: Each well was transfected with 1 μg of pTVT7_AKAV-7_L, 1 μg of pTVT7_AKAV-7_M, 1 μg of pTVT7_AKAV-7_S, 0.5 μg of pTM1_AKAV-7_RdRp, and 0.5 μg of pTM1_AKAV-7_N using 2 μL of Lipofectamine-3000 Transfection Reagent (Invitrogen, USA) per μg of DNA in 500 μL of Opti-MEM (Gibco, USA). .. After 24 h of incubation at 37 °C, the Opti-MEM was replaced with 500 μL of MEM containing 1% antibiotic/antimycotic solution (Gibco, USA), 3% FBS, and 10% TPB (BD, Difco, USA). ..

Isolation:

Article Title: Propagation of a Brazilian isolate of Anaplasma marginale with appendage in a tick cell line (BME26) derived from Rhipicephalus (Boophilus) microplus.
Article Snippet: Anaplasma marginale is a tick-borne pathogen of cattle responsible for the disease anaplasmosis.. Data suggest that Rhipicephalus (Boophilus) microplus and R. annulatus may be the major tick vectors of A. marginale in tropical and subtropical regions of the world.. In this work we demonstrated the first infection and propagation of a Brazilian isolate of A. marginale (UFMG1) in the BME26 cell line derived originally from embryos of R. (Boophilus) microplus.