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poly(i:c) (hmw) lyovec  (InvivoGen)


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    InvivoGen poly(i:c) (hmw) lyovec
    Poly(i:C) (Hmw) Lyovec, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 17180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlrl/Poly(I%3AC)+(HMW)+LyoVec/custom%40tlrl-piclv%4042787837
    Average 99 stars, based on 17180 article reviews
    poly(i:c) (hmw) lyovec - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Chitin oligomers induce atypical NLRP3 inflammasome activation and innate immune training
    Article Snippet: Prior to use for sterile stimulation, chitin and chitosan oligomers were suspended in endotoxin free water and tested for endotoxin level by using the limulus amebocyte lysate (LAL) assay ToxinSensor TM chromogenic LAL endotoxin assay kit (Cat# L00350C, Genscript). .. Cells were primed with ultrapure LPS-EB (Cat# tlrl-3lpsb; InvivoGen) or Pam3Cat# tlrl-pms; InvivoGen) at 100 ng/mL, and inflammasome activation was induced by the addition of 10 μM nigericin (Cat# tlrl-nig; InvivoGen) 1h prior to supernatant collection. .. For immune training experiments, chitin oligomers (DP2–5, DP6–9, DP10–15, DP16–20, and dispersible whole glucan particles (WGP; Cat# tlrl-wgp; InvivoGen)) were applied at a training concentration of 100 μg/mL.

    Red Blood Cell Lysis:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Isolation:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Article Title: Combined type 2 cytokine blockade enhances PD-1-mediated antitumor immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-Gata3 PE-CF594 BD Cat# 563510; Clone: L50-823; RRID: AB_2738248 Perforin APC Biolegend Cat# 154304; Clone: S16009A; RRID: AB_2721463 Chemicals, peptides, and recombinant proteins Mitomycin C Stemcell Technologies Cat# 73274 Ficoll-Paque Plus Cytiva Cat# 17144002 Recombinant human IL-2 improved sequence Miltenyi Biotec Cat# 130-097-743 Recombinant human IL-7 Miltenyi Biotec Cat# 130-095-367 Recombinant human IL-4 Biolegend Cat# 574006 Recombinant human IL-12 R&D Systems Cat# 10018-IL-050 Recombinant murine GM-CSF Biolegend Cat# 576306 Recombinant murine Flt3L Peprotech Cat# 250-31L LPS-EB Invivogen Cat# tlrl-eblps Recombinant murine IL-4 Biolegend Cat# 574306 Recombinant murine IL-13 Biolegend Cat# 575906 Recombinant murine IL-1β Biolegend Cat# 575106 Recombinant murine TNFα Biolegend Cat# 575206 Recombinant murine TSLP R&D Systems Cat# 1398-TS Critical commercial assays EasySepTM Human CD4+ T cell Isolation Kit Stemcell Technologies Cat# 100-0696 EasySepTM Human CD8+ T cell Isolation Kit Stemcell Technologies Cat# 100-0710 EasySepTM Human Monocyte Isolation Kit Stemcell Technologies Cat# 100-0697 V-PLEX Human IL-13 Kit Meso Scale Diagnostics Cat# K151ODD V-PLEX Human IFN-γ Kit Meso Scale Diagnostics Cat# K151QOD LEGENDplexTM Human Cytokine Panel 2 Biolegend Cat# 741377 LEGENDplexTM Mouse Cytokine Panel 2 Biolegend Cat# 741334 LEGENDplexTM Mouse Th Cytokine Panel Biolegend Cat# 741044 LEGENDplexTM Mouse HSC Panel Biolegend Cat# 740677 LEGENDplexTM Mouse Proinflammatory Chemokine Panel 1 Biolegend Cat# 741294 Deposited data Gencode mouse reference genome Gencode vM17 TCGA Pan-Cancer Atlas data NCI Genomic Data Commons Published dataset Public murine tumor model RNA-seq data Sequence Read Archive SRA: PRJNA505989 CT26 Tumor RNA-seq data GEO GEO: GSE342349 Experimental models: Cell lines Human melanoma cells expressing nuclear-restricted GFP Pfizer, Inc. Internal ID: A375-nuGFP Murine colon adenocarcinoma cells Pfizer, Inc. Internal ID: CT26 Murine lung adenocarcinoma cells Pfizer, Inc. Internal ID: KP787 Experimental models: Organisms/strains BALB/cJ mice Jackson Laboratories Cat# 000651; Female, 6–8 weeks of age C57BL/6 mice Jackson Laboratories Cat# 000664; Female, 6–8 weeks of age Software and algorithms GraphPad Prism GraphPad v11.0.2 Data4Cure Biomedical Intelligence Cloud Data4Cure, Inc. https://www.data4cure.com STAR aligner Dobin et al./STAR v020201 RSEM RSEM v1.3.0 bcl2fastq2 Illumina v2.20 (Continued on next page) 18 Cell Reports 45, 117910, September 22, 2026 .. PBMCs were isolated from healthy donors by density gradient centrifugation over Ficoll-Paque Plus (Cytiva) and cryopreserved before use.

    LDH Cytotoxicity Assay:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Reverse Transcription:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Polymerase Chain Reaction:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Gene Expression:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Cell Culture:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    XF Assay:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Software:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    FACS:

    Article Title: Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase D Sigma-Aldrich/Roche Cat# 11088858001 DNAse I Sigma-Aldrich Cat# 11284932001 LPS (Lipopolysaccharide) Invivogen Cat# tlrl-pb5lps PAM3CSK4 Invivogen Cat# tlrl-pms PMA (Phorbol 12-myristate 13-acetate) Sigma-Aldrich Cat# 524400 Ionomycin Sigma-Aldrich Cat# I3909 Brefeldin A (BFA) Sigma-Aldrich Cat# B7651 DTAF (5-(4,6-Dichlorotriazinyl)aminofluorescein) Sigma-Aldrich Cat# D0531 Borate (sodium tetraboarate decahydrate) Sigma-Aldrich Cat# S9640 Oligomycin Sigma-Aldrich Cat# 495455 FCCP Agilent Rotenone Agilent Antimycin A Agilent 2-Deoxyglucose (2-DG) Sigma-Aldrich Cat# D8375 Red blood cell lysis buffer Sigma-Aldrich Cat# R7757 CellTrace Violet Invitrogen Cat# C34557 pHrodo Green E. coli BioParticles Invitrogen Cat# P35366 Dimethyl Malate (DMM) Sigma-Aldrich Cat# S379239 Critical commercial assays Mouse Monocyte Isolation Kit Miltenyi Biotec Cat# 130-100-629 Mouse cKit Isolation Kit Miltenyi Biotec Cat# 130-097-146 LDH Cytotoxicity Assay Kit Thermo Fisher Cat# C20300 PureLink RNA Mini Kit Invitrogen Cat# 12183018A High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat# 4374967 TaqMan Universal PCR Master Mix Thermo Fisher Cat# 4304437 TaqMan Pre-designed Gene Expression Assay Thermo Fisher See Table S1 for individual assay id’s Mouse TNF ELISA kit Invitrogen Cat# 88-7324-77 Mouse IL6 ELISA kit Invitrogen Cat# 88-7064-88 QuantiBlue HEK-BlueTM Detection reagent Invivogen hb-det3 Seahorse XFe96/XF Pro Cell Culture Microplates Agilent 103794–100 Seahorse XF DMEM assay medium pack, pH 7.4 Agilent 103680–100 Experimental models: cell lines B16-F10 mouse melanoma Finlay Lab, TCD N/A MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A GFP-MC38 mouse colon adenocarcinoma Lydia Lynch, TCD N/A EO771 mouse breast cancer cell line Finlay Lab, TCD N/A HEK-Blue hDectin1a Invivogen hkb-hdect1a Experimental models: organisms/strains Mouse: C57BL/6J Charles River/In-house breeding RRID: IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) In-house breeding N/A Software and algorithms FlowJo v10 BD Biosciences RRID: SCR_008520 GraphPad Prism 9.3.0 GraphPad Software RRID: SCR_002798 Seahorse Wave software Agilent RRID: SCR_014526 Microsoft Office Microsoft www.microsoft.com R studio R Studio 2020 Team http://www.rstudio.com/ FACS Diva BD Biosciences V9.0 (Continued on next page) Cell Reports 45, 117648, July 28, 2026 17 .. C57BL/6J and CD45.1 mice aged 10–12 weeks were bred in-unit or purchased from Charles River Laboratories.

    other:

    Article Title: Reversal of T cell exhaustion enables cancer vaccine efficacy in BRCA1 -deficient ovarian cancer
    Article Snippet: LPS-EB , Invivogen , Cat # tlrl-eblps.

    Recombinant:

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Article Title: CD163 + red pulp macrophages interact with marginal metallophilic macrophages during blood-stage malaria to maintain splenic architecture.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER XCR1 – APC-Cy7 (clone ZET) Biolegend Cat# 148224; RRID: AB_2783118 α-a.Hamster – DyLight 649 (clone Poly4055) Biolegend Cat# 405505; RRID: AB_1575122 α-chicken – AF488 Thermo Fisher Scientific Cat# A78948; RRID: AB_2921070 α-rabbit – AF488 Thermo Fisher Scientific Cat# A-11008; RRID: AB_143165 α-rat – AF488 Thermo Fisher Scientific Cat# A-11006; RRID: AB_2534074 α-rat – AF555 Thermo Fisher Scientific Cat# A78945; RRID: AB_2910652 α-rat – AF647 Biolegend Cat# A78947 CD163 – ATTO-647 Anders Etzerodt CD163 (clone TNKUPJ) Thermo Fisher Scientific Cat# 14-1631-82; RRID: AB_2716934 CD163 – PE (clone S15049I) Biolegend Cat# 155308; RRID: AB_2814062 CD169 (Signlec-1) – Biotin (clone REA197 | 3D6.112) Miltenyi Biotec Cat# 130-121-441; RRID: AB_2889469 CD31 – AF488 (clone MEC13.3) Biolegend Cat# 102514; RRID: AB_2161031 CD31 (armenian Hamster) – AF488 (clone 2H8) Thermo Fisher Scientific Cat# MA3105; RRID: AB_223592 F4/80 (chicken, clone EPR26545-166) Abcam Cat# ab320060 F4/80 (rat, clone CI:A3-1) BIO-RAD Cat# MCA497GA; RRID: AB_323806 Iba1 (rabbit, clone EPR16589) Abcam Cat# ab178847; RRID: AB_2832244 Streptavidin - PE Biolegend Cat# 405203 Streptavidin – AF647 Biolegend Cat# 405237 GFP (Rat, clone GF090R) Biotechnik Gerbu Cat# 04404 Bacterial and virus strains LCMV R. Ahmed, Emory University NCBITaxon_11624 LPS (from E.coli 0111:B4) InvivoGen Cat# tlrl-eblps Biological samples Plasmodium chabaudi AS Kevin Couper, Manchester NCBITaxon_31271 Plasmodium yoelii 17XNL QIMR Brisbane NCBITaxon_1323249 Plasmodium berghei ANKA Walter and Eliza Hall Institute NCBITaxon_5823 Chemicals, peptides, and recombinant proteins Phenyl-hydrazine Sigma-Aldrich Cat# P26252 Tamoxifen Sigma-Aldrich Cat# T5648 Chloroquine Sigma-Aldrich Cat# C6628 Hoechst ThermoFisher Scientific Cat# H1398 DAPI Biolegend Cat# 42281 DNase Sigma-Aldrich Cat# DN25 Dispase Gibco Cat# 17105041 Collagenase D Roche Cat# 11088858001 Entellan Millipore Cat# 1.07961.0100 Formaldehyde ThermoFisher Scientific Cat# 11586711 D-Sucrose ThermoFisher Scientific Cat# 10638403 Tissue-Tek O.C.T. .. Sakura Finetek Cat# 4583 Triton X-100 Sigma-Aldrich Cat# X100 BSA ThermoFisher Scientific Cat# 37525 Donkey-serum Sigma-Aldrich Cat# D9663 Flavopiridol Sigma-Aldrich Cat# F3055 Ascorbic acid AppliChem Cat# A1052,0100 Bathophenanthroline disulfonic acid (C24H16N2O6S2) Sigma-Aldrich Cat# B1375-500MG Hydrogen Chloride (HCl) VWR Cat# 20252.290 Iron (II) sulfate heptahydrate (FeH14O11S) Sigma-Aldrich Cat# F-8633 Sodium acetate (NaOAc) (CH3COONa) Sigma-Aldrich Cat# S2889-250G Trichloroacetic acid (TCA) (C2HCl3O2) Sigma-Aldrich Cat# 1.00807.0100 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–19.e1–e8, October 13, 2026 e2

    Article Title: Combined type 2 cytokine blockade enhances PD-1-mediated antitumor immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-Gata3 PE-CF594 BD Cat# 563510; Clone: L50-823; RRID: AB_2738248 Perforin APC Biolegend Cat# 154304; Clone: S16009A; RRID: AB_2721463 Chemicals, peptides, and recombinant proteins Mitomycin C Stemcell Technologies Cat# 73274 Ficoll-Paque Plus Cytiva Cat# 17144002 Recombinant human IL-2 improved sequence Miltenyi Biotec Cat# 130-097-743 Recombinant human IL-7 Miltenyi Biotec Cat# 130-095-367 Recombinant human IL-4 Biolegend Cat# 574006 Recombinant human IL-12 R&D Systems Cat# 10018-IL-050 Recombinant murine GM-CSF Biolegend Cat# 576306 Recombinant murine Flt3L Peprotech Cat# 250-31L LPS-EB Invivogen Cat# tlrl-eblps Recombinant murine IL-4 Biolegend Cat# 574306 Recombinant murine IL-13 Biolegend Cat# 575906 Recombinant murine IL-1β Biolegend Cat# 575106 Recombinant murine TNFα Biolegend Cat# 575206 Recombinant murine TSLP R&D Systems Cat# 1398-TS Critical commercial assays EasySepTM Human CD4+ T cell Isolation Kit Stemcell Technologies Cat# 100-0696 EasySepTM Human CD8+ T cell Isolation Kit Stemcell Technologies Cat# 100-0710 EasySepTM Human Monocyte Isolation Kit Stemcell Technologies Cat# 100-0697 V-PLEX Human IL-13 Kit Meso Scale Diagnostics Cat# K151ODD V-PLEX Human IFN-γ Kit Meso Scale Diagnostics Cat# K151QOD LEGENDplexTM Human Cytokine Panel 2 Biolegend Cat# 741377 LEGENDplexTM Mouse Cytokine Panel 2 Biolegend Cat# 741334 LEGENDplexTM Mouse Th Cytokine Panel Biolegend Cat# 741044 LEGENDplexTM Mouse HSC Panel Biolegend Cat# 740677 LEGENDplexTM Mouse Proinflammatory Chemokine Panel 1 Biolegend Cat# 741294 Deposited data Gencode mouse reference genome Gencode vM17 TCGA Pan-Cancer Atlas data NCI Genomic Data Commons Published dataset Public murine tumor model RNA-seq data Sequence Read Archive SRA: PRJNA505989 CT26 Tumor RNA-seq data GEO GEO: GSE342349 Experimental models: Cell lines Human melanoma cells expressing nuclear-restricted GFP Pfizer, Inc. Internal ID: A375-nuGFP Murine colon adenocarcinoma cells Pfizer, Inc. Internal ID: CT26 Murine lung adenocarcinoma cells Pfizer, Inc. Internal ID: KP787 Experimental models: Organisms/strains BALB/cJ mice Jackson Laboratories Cat# 000651; Female, 6–8 weeks of age C57BL/6 mice Jackson Laboratories Cat# 000664; Female, 6–8 weeks of age Software and algorithms GraphPad Prism GraphPad v11.0.2 Data4Cure Biomedical Intelligence Cloud Data4Cure, Inc. https://www.data4cure.com STAR aligner Dobin et al./STAR v020201 RSEM RSEM v1.3.0 bcl2fastq2 Illumina v2.20 (Continued on next page) 18 Cell Reports 45, 117910, September 22, 2026 .. PBMCs were isolated from healthy donors by density gradient centrifugation over Ficoll-Paque Plus (Cytiva) and cryopreserved before use.

    Staining:

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Diff-Quik:

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Viability Assay:

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    RNA Sequencing:

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Virus:

    Article Title: CD163 + red pulp macrophages interact with marginal metallophilic macrophages during blood-stage malaria to maintain splenic architecture.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER XCR1 – APC-Cy7 (clone ZET) Biolegend Cat# 148224; RRID: AB_2783118 α-a.Hamster – DyLight 649 (clone Poly4055) Biolegend Cat# 405505; RRID: AB_1575122 α-chicken – AF488 Thermo Fisher Scientific Cat# A78948; RRID: AB_2921070 α-rabbit – AF488 Thermo Fisher Scientific Cat# A-11008; RRID: AB_143165 α-rat – AF488 Thermo Fisher Scientific Cat# A-11006; RRID: AB_2534074 α-rat – AF555 Thermo Fisher Scientific Cat# A78945; RRID: AB_2910652 α-rat – AF647 Biolegend Cat# A78947 CD163 – ATTO-647 Anders Etzerodt CD163 (clone TNKUPJ) Thermo Fisher Scientific Cat# 14-1631-82; RRID: AB_2716934 CD163 – PE (clone S15049I) Biolegend Cat# 155308; RRID: AB_2814062 CD169 (Signlec-1) – Biotin (clone REA197 | 3D6.112) Miltenyi Biotec Cat# 130-121-441; RRID: AB_2889469 CD31 – AF488 (clone MEC13.3) Biolegend Cat# 102514; RRID: AB_2161031 CD31 (armenian Hamster) – AF488 (clone 2H8) Thermo Fisher Scientific Cat# MA3105; RRID: AB_223592 F4/80 (chicken, clone EPR26545-166) Abcam Cat# ab320060 F4/80 (rat, clone CI:A3-1) BIO-RAD Cat# MCA497GA; RRID: AB_323806 Iba1 (rabbit, clone EPR16589) Abcam Cat# ab178847; RRID: AB_2832244 Streptavidin - PE Biolegend Cat# 405203 Streptavidin – AF647 Biolegend Cat# 405237 GFP (Rat, clone GF090R) Biotechnik Gerbu Cat# 04404 Bacterial and virus strains LCMV R. Ahmed, Emory University NCBITaxon_11624 LPS (from E.coli 0111:B4) InvivoGen Cat# tlrl-eblps Biological samples Plasmodium chabaudi AS Kevin Couper, Manchester NCBITaxon_31271 Plasmodium yoelii 17XNL QIMR Brisbane NCBITaxon_1323249 Plasmodium berghei ANKA Walter and Eliza Hall Institute NCBITaxon_5823 Chemicals, peptides, and recombinant proteins Phenyl-hydrazine Sigma-Aldrich Cat# P26252 Tamoxifen Sigma-Aldrich Cat# T5648 Chloroquine Sigma-Aldrich Cat# C6628 Hoechst ThermoFisher Scientific Cat# H1398 DAPI Biolegend Cat# 42281 DNase Sigma-Aldrich Cat# DN25 Dispase Gibco Cat# 17105041 Collagenase D Roche Cat# 11088858001 Entellan Millipore Cat# 1.07961.0100 Formaldehyde ThermoFisher Scientific Cat# 11586711 D-Sucrose ThermoFisher Scientific Cat# 10638403 Tissue-Tek O.C.T. .. Sakura Finetek Cat# 4583 Triton X-100 Sigma-Aldrich Cat# X100 BSA ThermoFisher Scientific Cat# 37525 Donkey-serum Sigma-Aldrich Cat# D9663 Flavopiridol Sigma-Aldrich Cat# F3055 Ascorbic acid AppliChem Cat# A1052,0100 Bathophenanthroline disulfonic acid (C24H16N2O6S2) Sigma-Aldrich Cat# B1375-500MG Hydrogen Chloride (HCl) VWR Cat# 20252.290 Iron (II) sulfate heptahydrate (FeH14O11S) Sigma-Aldrich Cat# F-8633 Sodium acetate (NaOAc) (CH3COONa) Sigma-Aldrich Cat# S2889-250G Trichloroacetic acid (TCA) (C2HCl3O2) Sigma-Aldrich Cat# 1.00807.0100 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–19.e1–e8, October 13, 2026 e2

    Sequencing:

    Article Title: Combined type 2 cytokine blockade enhances PD-1-mediated antitumor immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-Gata3 PE-CF594 BD Cat# 563510; Clone: L50-823; RRID: AB_2738248 Perforin APC Biolegend Cat# 154304; Clone: S16009A; RRID: AB_2721463 Chemicals, peptides, and recombinant proteins Mitomycin C Stemcell Technologies Cat# 73274 Ficoll-Paque Plus Cytiva Cat# 17144002 Recombinant human IL-2 improved sequence Miltenyi Biotec Cat# 130-097-743 Recombinant human IL-7 Miltenyi Biotec Cat# 130-095-367 Recombinant human IL-4 Biolegend Cat# 574006 Recombinant human IL-12 R&D Systems Cat# 10018-IL-050 Recombinant murine GM-CSF Biolegend Cat# 576306 Recombinant murine Flt3L Peprotech Cat# 250-31L LPS-EB Invivogen Cat# tlrl-eblps Recombinant murine IL-4 Biolegend Cat# 574306 Recombinant murine IL-13 Biolegend Cat# 575906 Recombinant murine IL-1β Biolegend Cat# 575106 Recombinant murine TNFα Biolegend Cat# 575206 Recombinant murine TSLP R&D Systems Cat# 1398-TS Critical commercial assays EasySepTM Human CD4+ T cell Isolation Kit Stemcell Technologies Cat# 100-0696 EasySepTM Human CD8+ T cell Isolation Kit Stemcell Technologies Cat# 100-0710 EasySepTM Human Monocyte Isolation Kit Stemcell Technologies Cat# 100-0697 V-PLEX Human IL-13 Kit Meso Scale Diagnostics Cat# K151ODD V-PLEX Human IFN-γ Kit Meso Scale Diagnostics Cat# K151QOD LEGENDplexTM Human Cytokine Panel 2 Biolegend Cat# 741377 LEGENDplexTM Mouse Cytokine Panel 2 Biolegend Cat# 741334 LEGENDplexTM Mouse Th Cytokine Panel Biolegend Cat# 741044 LEGENDplexTM Mouse HSC Panel Biolegend Cat# 740677 LEGENDplexTM Mouse Proinflammatory Chemokine Panel 1 Biolegend Cat# 741294 Deposited data Gencode mouse reference genome Gencode vM17 TCGA Pan-Cancer Atlas data NCI Genomic Data Commons Published dataset Public murine tumor model RNA-seq data Sequence Read Archive SRA: PRJNA505989 CT26 Tumor RNA-seq data GEO GEO: GSE342349 Experimental models: Cell lines Human melanoma cells expressing nuclear-restricted GFP Pfizer, Inc. Internal ID: A375-nuGFP Murine colon adenocarcinoma cells Pfizer, Inc. Internal ID: CT26 Murine lung adenocarcinoma cells Pfizer, Inc. Internal ID: KP787 Experimental models: Organisms/strains BALB/cJ mice Jackson Laboratories Cat# 000651; Female, 6–8 weeks of age C57BL/6 mice Jackson Laboratories Cat# 000664; Female, 6–8 weeks of age Software and algorithms GraphPad Prism GraphPad v11.0.2 Data4Cure Biomedical Intelligence Cloud Data4Cure, Inc. https://www.data4cure.com STAR aligner Dobin et al./STAR v020201 RSEM RSEM v1.3.0 bcl2fastq2 Illumina v2.20 (Continued on next page) 18 Cell Reports 45, 117910, September 22, 2026 .. PBMCs were isolated from healthy donors by density gradient centrifugation over Ficoll-Paque Plus (Cytiva) and cryopreserved before use.

    Cell Isolation:

    Article Title: Combined type 2 cytokine blockade enhances PD-1-mediated antitumor immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-Gata3 PE-CF594 BD Cat# 563510; Clone: L50-823; RRID: AB_2738248 Perforin APC Biolegend Cat# 154304; Clone: S16009A; RRID: AB_2721463 Chemicals, peptides, and recombinant proteins Mitomycin C Stemcell Technologies Cat# 73274 Ficoll-Paque Plus Cytiva Cat# 17144002 Recombinant human IL-2 improved sequence Miltenyi Biotec Cat# 130-097-743 Recombinant human IL-7 Miltenyi Biotec Cat# 130-095-367 Recombinant human IL-4 Biolegend Cat# 574006 Recombinant human IL-12 R&D Systems Cat# 10018-IL-050 Recombinant murine GM-CSF Biolegend Cat# 576306 Recombinant murine Flt3L Peprotech Cat# 250-31L LPS-EB Invivogen Cat# tlrl-eblps Recombinant murine IL-4 Biolegend Cat# 574306 Recombinant murine IL-13 Biolegend Cat# 575906 Recombinant murine IL-1β Biolegend Cat# 575106 Recombinant murine TNFα Biolegend Cat# 575206 Recombinant murine TSLP R&D Systems Cat# 1398-TS Critical commercial assays EasySepTM Human CD4+ T cell Isolation Kit Stemcell Technologies Cat# 100-0696 EasySepTM Human CD8+ T cell Isolation Kit Stemcell Technologies Cat# 100-0710 EasySepTM Human Monocyte Isolation Kit Stemcell Technologies Cat# 100-0697 V-PLEX Human IL-13 Kit Meso Scale Diagnostics Cat# K151ODD V-PLEX Human IFN-γ Kit Meso Scale Diagnostics Cat# K151QOD LEGENDplexTM Human Cytokine Panel 2 Biolegend Cat# 741377 LEGENDplexTM Mouse Cytokine Panel 2 Biolegend Cat# 741334 LEGENDplexTM Mouse Th Cytokine Panel Biolegend Cat# 741044 LEGENDplexTM Mouse HSC Panel Biolegend Cat# 740677 LEGENDplexTM Mouse Proinflammatory Chemokine Panel 1 Biolegend Cat# 741294 Deposited data Gencode mouse reference genome Gencode vM17 TCGA Pan-Cancer Atlas data NCI Genomic Data Commons Published dataset Public murine tumor model RNA-seq data Sequence Read Archive SRA: PRJNA505989 CT26 Tumor RNA-seq data GEO GEO: GSE342349 Experimental models: Cell lines Human melanoma cells expressing nuclear-restricted GFP Pfizer, Inc. Internal ID: A375-nuGFP Murine colon adenocarcinoma cells Pfizer, Inc. Internal ID: CT26 Murine lung adenocarcinoma cells Pfizer, Inc. Internal ID: KP787 Experimental models: Organisms/strains BALB/cJ mice Jackson Laboratories Cat# 000651; Female, 6–8 weeks of age C57BL/6 mice Jackson Laboratories Cat# 000664; Female, 6–8 weeks of age Software and algorithms GraphPad Prism GraphPad v11.0.2 Data4Cure Biomedical Intelligence Cloud Data4Cure, Inc. https://www.data4cure.com STAR aligner Dobin et al./STAR v020201 RSEM RSEM v1.3.0 bcl2fastq2 Illumina v2.20 (Continued on next page) 18 Cell Reports 45, 117910, September 22, 2026 .. PBMCs were isolated from healthy donors by density gradient centrifugation over Ficoll-Paque Plus (Cytiva) and cryopreserved before use.



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    Reagent list and references.

    Journal: Autophagy Reports

    Article Title: LC3-associated phagocytosis is impaired in monocyte-derived macrophages from systemic sclerosis patients

    doi: 10.1080/27694127.2026.2730912

    Figure Lengend Snippet: Reagent list and references.

    Article Snippet: Phorbol myristate acetate , 50 ng/mL , Invivogen, tlrl-pma.

    Techniques: Concentration Assay, Cell Isolation, Luminex, Glass, Flow Cytometry

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    Journal: PLOS Biology

    Article Title: Uracil-driven ROS signaling and larval TrpA1-B neuron activation are required for priming the adult Drosophila gustatory response to bacteria

    doi: 10.1371/journal.pbio.3004042

    Figure Lengend Snippet: (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli K12 (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .

    Article Snippet: PGN is E. coli K12: Invivogen, catalog code #tlrl-kipgn.

    Techniques: Bacteria, Control, RNAi Assay, Activity Assay, Inactivation, Expressing, Concentration Assay