Journal: Aging and Disease
Article Title: Bone Regeneration Enhanced by Quercetin-Capped Selenium Nanoparticles via miR206/Connexin43, WNT, and BMP signaling pathways
doi: 10.14336/AD.2025.0025
Figure Lengend Snippet: Qu-SeNPs activate WNT and BMP signaling pathways in osteoblasts . ( A ) Transiently transfected MC3T3-E1 cells with Axin-2 and (B) BRE reporter construct were treated free Qu (1 µg mL -1 ) or Qu-SeNPs at equivalent Qu concentration (75 ng mL -1 ) (n = 4; *p<0.05, **p<0.01, ***p<0.001 vs. control; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05, ## p<0.01; unpaired t-test). Luciferase activities were assessed in cell lysates after 48 h and normalized to Renilla luciferase activity, as detailed in the Materials and Methods section. MC3T3-E1 cells were treated with free Qu (1 µg mL -1 ) or Qu-SeNPs at an equivalent Qu concentration (75 ng mL -1 ) for the time indicated in the figure. Protein lysates were subsequently collected. Western blotting revealed the stabilization of (C) β-catenin and the (D) phosphorylation of Smad 1/5/8 molecules. β-actin was a loading control and normalized for densitometric analysis of Western blot bands. The relative expression level is compared to the control (n = 3; *p<0.05, **p<0.01, ***p<0.001 vs. 0 h; one-way ANOVA with Dunnett’s multiple comparisons test, and # p<0.05 vs. free Qu at similar time points; unpaired t-test.
Article Snippet: Trizol reagent, SuperScript ІІ Reverse Transcriptase, and Renilla luciferase thymidine kinase construct was purchased from Invitrogen, USA.
Techniques: Protein-Protein interactions, Transfection, Construct, Concentration Assay, Control, Luciferase, Activity Assay, Western Blot, Phospho-proteomics, Expressing