Review



hsting fts binding assay examples  (InvivoGen)


Bioz Verified Symbol InvivoGen is a verified supplier
Bioz Manufacturer Symbol InvivoGen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    InvivoGen hsting fts binding assay examples
    Hsting Fts Binding Assay Examples, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP1-Dual+Cells/us12655124-2726-48-178
    Average 97 stars, based on 569 article reviews
    hsting fts binding assay examples - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    other:

    Article Title: Discovery of Pyridazine-3-carboxamide derivatives as potent STING agonists that suppress pseudorabies virus replication.
    Article Snippet: Background: The stimulator of interferon genes (STING) pathway is a critical component of the host defense against viral infections, representing an attractive target for developing broad-spectrum antiviral therapies.. While cyclic dinucleotide (CDN)-based STING agonists have been extensively explored, the development of nonCDN small-molecule agonists remains limited, particularly in veterinary applications.. Methods: To develop STING agonists with micromolar-scale agonistic activity, antiviral activity, stability and safety using a combination strategy.

    Article Title: Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation products
    Article Snippet: THP1-Dual hTLR9 cells (InvivoGen) overexpress the human TLR9 gene and are engineered with two secreted reporters.

    Article Title: Tuning the Composition of Spherical High-Density Lipoprotein-Like Nanoparticles Modulates in Vitro Function
    Article Snippet: THP1-Dual cells (100,000 cells/well) obtained from InvivoGen (San Diego, CA) were seeded in 96-well plates and simultaneously treated with media containing the appropriate oc-HDL NP with 1 ng/mL lipopolysaccharide (LPS; O55/B5) from Sigma-Aldrich (St. Louis, MO).

    Article Title: Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation products
    Article Snippet: THP1-Dual hTLR9 cells (InvivoGen, thpd-htlr9) were grown according to the manufacturer’s instructions in a maintenance medium of Roswell Park Memorial Institute (RPMI) 1640 Medium + L-Glutamine (Gibco, 11875093) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Gibco, A5256801), 25 mM HEPES (Gibco, 15630080), 100 U/mL penicillin and streptomycin solution (Cytiva, SV30010), 100 μg/mL normocin (InvivoGen, ant-nr-05), under standard conditions of 37°C, 5% CO 2 .

    Concentration Assay:

    Article Title: Carbohydrate fatty acid monosulphate ester adjuvant enhances the immunogenicity of influenza antigens via TLR4/2-dependent mechanisms.
    Article Snippet: After staining the cells for the surface marker CD4, and CD127, fixation/ permeabilization was carried out with an intracellular fixation & permeabilization buffer (eBioscience) for intracellular staining of IFN-g, IL-17A, IL-4, FoxP3 to determine the frequency of Th1, Th2, Th17, and Treg subpopulations by flow cytometry. .. THP1-Dual, THP1-Dual MD2-CD14-TLR4, and THP1-Dual KO-TLR4 reporter cells (Invivogen) were resuspended at a concentration of 100,000 cells per well in a 96-well U-bottom plate in 200 μl RPMI 1640 Medium, GlutaMAX Supplement (Gibco), supplemented with 25mM HEPES, 10% FBS, 100 IU/ml penicillin, and 100 μg/ml streptomycin. ..

    Cell Culture:

    Article Title: Nucleic acid nanostructure platform for programming immune stimulation
    Article Snippet: A fluorescent calibration curve was produced using serial dilutions of the free CpG overhang hybridized to the Cy5-conjugated ssDNA oligo and measured with the Tecan Spark to produce a regression line with which the coverage of CpGs on each NANP variant was determined. .. Reporter Cell Assays HEK-Blue TLR9 reporter cells and THP1-Dual reporter cells were purchased from Invivogen and cultured according to manufacturer protocols. .. One day prior to cell assays, TLR9 cells were detached from the flask, centrifuged at 250×g for 5 minutes at room temperature and resuspended in fresh growth medium, consisting of Dulbecco's Modified Eagle Medium with 4.5 g/L glucose (Sigma-Aldrich, cat. Code D6546-6×500ML), 10% heat-inactivated FBS (Fisher Scientific, cat. Code SH3007002HI), 100 U/mL penicillin, 100 ug/mL streptomycin (Life Technologies, cat. Code 15140148), 100 ug/mL Normocin (manufacturer, cat. Code) and 2 mM L-glutamine (Fisher Scientific, cat. Code SH3003401).

    Article Title: Glehnia littoralis and imperatorin attenuate allergic airway inflammation via mast cell stabilization and transcriptome-defined suppression of FcεRI/TLR-MAPK/NF-κB signaling.
    Article Snippet: 20 Background: Allergic airway inflammation is driven by mast cell activation and IgE–FcεRI 21 signaling.. Current pharmacological treatments often present limited efficacy or long-term 22 adverse effects.. Glehnia littoralis Fr.

    Article Title: Design, Synthesis, and Pharmacological Evaluation of cGAS/HDAC Dual Inhibitors for Treatment of Autoimmune Diseases.
    Article Snippet: cGAS overactivation is linked to various inflammatory and autoimmune diseases.. Recent studies identify HDACs as critical regulators of cGAS, suggesting that dual cGAS/HDAC inhibition could be a novel therapeutic strategy.. Herein, we report the identification of an HDAC/cGAS dual inhibitor, 31h, containing a hydroxamic acid moiety.



    Similar Products

    monocytic cell line thp1  (ATCC)
    99
    ATCC monocytic cell line thp1
    Monocytic Cell Line Thp1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP-1/pm42316966-59-9-16
    Average 99 stars, based on 1 article reviews
    monocytic cell line thp1 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    hsting fts binding assay examples  (InvivoGen)
    97
    InvivoGen hsting fts binding assay examples
    Hsting Fts Binding Assay Examples, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP1-Dual+Cells/us12655124-2726-48-178
    Average 97 stars, based on 1 article reviews
    hsting fts binding assay examples - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    hek-blue htlr9 cells  (InvivoGen)
    96
    InvivoGen hek-blue htlr9 cells
    Hek Blue Htlr9 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/HEK-Blue+hTLR9+cells/custom%40hkb-htlr9%4042320433
    Average 96 stars, based on 1 article reviews
    hek-blue htlr9 cells - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    thp1  (InvivoGen)
    97
    InvivoGen thp1
    Thp1, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP1-Dual+Cells/pm42268702-582-5-13
    Average 97 stars, based on 1 article reviews
    thp1 - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    thp1 cells  (ATCC)
    99
    ATCC thp1 cells
    Thp1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP-1/pm42269215-114-13-7
    Average 99 stars, based on 1 article reviews
    thp1 cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    thp1 human monocytic  (ATCC)
    99
    ATCC thp1 human monocytic
    Thp1 Human Monocytic, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP-1/pm42300103-147-0-17
    Average 99 stars, based on 1 article reviews
    thp1 human monocytic - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    thp1 dual thpd nfis  (InvivoGen)
    97
    InvivoGen thp1 dual thpd nfis
    Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL <t>(THP1),</t> or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.
    Thp1 Dual Thpd Nfis, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1/THP1-Dual+Cells/pmc13253205-177-3-14
    Average 97 stars, based on 1 article reviews
    thp1 dual thpd nfis - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL (THP1), or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.

    Journal: Materials Today Bio

    Article Title: Polyelectrolyte nanoparticles enable intracellular delivery of STING protein fragments for ovarian cancer immunotherapy

    doi: 10.1016/j.mtbio.2026.103251

    Figure Lengend Snippet: Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL (THP1), or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.

    Article Snippet: RAW-Lucia ISG (rawl-isg), THP1-Dual (thpd-nfis), and HEK-Blue mTLR4 (hkb-mtlr4) reporter cells were purchased from InvivoGen.

    Techniques: Activation Assay, Zeta Potential Analyzer, Control, Standard Deviation