Journal: Molecular Neurobiology
Article Title: A Peptide Motif Covering Splice Site B in Neuroligin-1 Binds to Aβ and Acts as a Neprilysin Inhibitor
doi: 10.1007/s12035-024-04475-z
Figure Lengend Snippet: NL1 and neurolide effects on neprilysin activity. Both NL1 ( A ) and neurolide ( B ) decreased the cleavage activity of recombinant neprilysin in a dose-dependent manner. C Neurolide, but not other NL1-derived peptides, NLp4 and NLp7, down regulates the activity of recombinant neprilysin. D Neurolide (10 μM) downregulates the activity of recombinant neprilysin in the presence of Aβ 1-42 applied either in 0.5 μM or 5 μM. At A – D panels data from four to five independent experiments are shown and compared with vehicle alone. E Both NL1 and neurolide downregulate the activity of neprilysin, extracted from brains of WT mice ( n = 5). Treatment of brain extracts with Thiorphan (100 nM) confirm the neprilysin activity in prepared brain fractions and used as a negative control. F Activity of neprilysin extracted from brains of 5XFAD mice ( n = 6) is significantly lower compared to WT mice ( n = 5) and is further significantly attenuated by treatment with 10 μM neurolide ( n = 6). In E – F , neprilysin activity in brain extracts was normalized to the activity of synthetic neprilysin in each experiment, set as 100% (dashed line). Values are mean ± SEM
Article Snippet: To determine the specificity of the assay, a set of samples were incubated with 10 μl of thiorphan (100 nM final concentration), a NEP-specific inhibitor (Anaspec) [ , ].
Techniques: Activity Assay, Recombinant, Derivative Assay, Negative Control
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