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Proteintech tbst
Tbst, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Proteintech
Average 99 stars, based on 299 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
99/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro
Article Snippet: .. Equivalent amounts of protein samples (20–30 μg) were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on an 8% gel and subsequently transferred to polyvinylidene difluoride (PVDF) membranes at 4°C, then blocked with 5% non-fat milk in TBST at room temperature for 2 h. The membranes were incubated with the following primary antibody at 4°C overnight: α-SMA (14395-1-AP, Proteintech, 1: 1000), Fibronectin (66042-1-lg, Proteintech, 1: 5000), CTGF (MD5912-50, Medical Discovery Leader, 1: 1000), Collagen III (MD4405-50, Medical Discovery Leader, 1: 1000), HO-1(66743-1-lg, Proteintech, 1: 1000), and Nrf2 (16396-1-AP, Proteintech, 1: 1000), Keap1(10503-2-AP, Proteintech, 1: 1000), collagen I (14695-1-AP, Proteintech, 1: 1000), MR (21854-1-AP, Proteintech, 1: 1000), LaminB1 (12987-1-AP, Proteintech, 1: 1000), Tubulin (10094-1-AP, Proteintech, 1: 10,000) and GAPDH (60004-1-lg, Proteintech, 1: 10000). .. After washing the membranes 3 times with 1×TBST, the membranes were incubated for 1.5 h at room temperature with Anti-mouse IgG (1: 10 000, PMK-014-095) and Anti-rabbit IgG (1: 10 000, PMK-014-090).

Incubation:

Article Title: Catalpol prevents denervated muscular atrophy related to the inhibition of autophagy and reduces BAX/BCL2 ratio via mTOR pathway
Article Snippet: .. Twenty-four hours after TBST [TBS with Tween 20 (0.1%)] rinsing, HRP secondary antibodies and anti-rabbit IgG HRP (Proteintech) were incubated with the immunoblot at a 1:5,000 dilution for 1 hour at room temperature. ..

Article Title: MicroRNA-148a overexpression improves the early development of porcine somatic cell nuclear transfer embryos
Article Snippet: .. Membranes were then washed 3 times for 10 min in TBST and then incubated for 1 h at RT with peroxidase-conjugated goat anti-mouse IgG (Protein Tech Group, Inc.; 1:3000). .. Membranes were then washed 3 times in TBST, visualized using enhanced chemiluminescence (ECL), and imaged using the ChemiDoc System (Bio-Rad).

Article Title: Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai
Article Snippet: .. In brief, the membrane was blocked with 5% skim milk in TBST at room temperature for 1 h. Then, the blots were incubated with primary antibodies against the NKA α subunit (1:500, ATP1A2 Rabbit Polyclonal, Cat. No. 16836-1-AP, Proteintech, Rosemont, IL, United States) overnight at 4°C, followed by incubation with appropriate peroxidase-conjugated secondary antibodies (1:3000, HRP Goat Anti-Rabbit IgG (H+L), Cat. No. AS014, ABclonal, Wuhan, China). .. Gray values were analyzed using ImageJ software.

Article Title: Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro
Article Snippet: .. Equivalent amounts of protein samples (20–30 μg) were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on an 8% gel and subsequently transferred to polyvinylidene difluoride (PVDF) membranes at 4°C, then blocked with 5% non-fat milk in TBST at room temperature for 2 h. The membranes were incubated with the following primary antibody at 4°C overnight: α-SMA (14395-1-AP, Proteintech, 1: 1000), Fibronectin (66042-1-lg, Proteintech, 1: 5000), CTGF (MD5912-50, Medical Discovery Leader, 1: 1000), Collagen III (MD4405-50, Medical Discovery Leader, 1: 1000), HO-1(66743-1-lg, Proteintech, 1: 1000), and Nrf2 (16396-1-AP, Proteintech, 1: 1000), Keap1(10503-2-AP, Proteintech, 1: 1000), collagen I (14695-1-AP, Proteintech, 1: 1000), MR (21854-1-AP, Proteintech, 1: 1000), LaminB1 (12987-1-AP, Proteintech, 1: 1000), Tubulin (10094-1-AP, Proteintech, 1: 10,000) and GAPDH (60004-1-lg, Proteintech, 1: 10000). .. After washing the membranes 3 times with 1×TBST, the membranes were incubated for 1.5 h at room temperature with Anti-mouse IgG (1: 10 000, PMK-014-095) and Anti-rabbit IgG (1: 10 000, PMK-014-090).

Article Title: Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia
Article Snippet: .. The following day, the membrane was incubated for 1 hr at room temperature with primary antibody solution in 1:20 western blocking reagent diluted in TBST at the following concentrations: rabbit polyclonal CIRBP (ProteinTech, 10209-2-AP, 1:1,000) and rabbit polyclonal HADHB (LSBio-LS-C334236, 1:2,500). .. After washing in TBST, the blots were incubated with the appropriate secondary antibody conjugated to horseradish peroxidase and detected with SuperSignal West Pico detection reagent (Thermo Scientific).

Article Title: MicroRNA-655-3p functions as a tumor suppressor by regulating ADAM10 and β-catenin pathway in Hepatocellular Carcinoma
Article Snippet: .. PVDF membranes were washed in TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (ProteinTech Group, USA). .. Antibody against GAPDH (Cell Signaling Technology, USA) was used as an internal control.

SDS Page:

Article Title: Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro
Article Snippet: .. Equivalent amounts of protein samples (20–30 μg) were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on an 8% gel and subsequently transferred to polyvinylidene difluoride (PVDF) membranes at 4°C, then blocked with 5% non-fat milk in TBST at room temperature for 2 h. The membranes were incubated with the following primary antibody at 4°C overnight: α-SMA (14395-1-AP, Proteintech, 1: 1000), Fibronectin (66042-1-lg, Proteintech, 1: 5000), CTGF (MD5912-50, Medical Discovery Leader, 1: 1000), Collagen III (MD4405-50, Medical Discovery Leader, 1: 1000), HO-1(66743-1-lg, Proteintech, 1: 1000), and Nrf2 (16396-1-AP, Proteintech, 1: 1000), Keap1(10503-2-AP, Proteintech, 1: 1000), collagen I (14695-1-AP, Proteintech, 1: 1000), MR (21854-1-AP, Proteintech, 1: 1000), LaminB1 (12987-1-AP, Proteintech, 1: 1000), Tubulin (10094-1-AP, Proteintech, 1: 10,000) and GAPDH (60004-1-lg, Proteintech, 1: 10000). .. After washing the membranes 3 times with 1×TBST, the membranes were incubated for 1.5 h at room temperature with Anti-mouse IgG (1: 10 000, PMK-014-095) and Anti-rabbit IgG (1: 10 000, PMK-014-090).

Blocking Assay:

Article Title: Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia
Article Snippet: .. The following day, the membrane was incubated for 1 hr at room temperature with primary antibody solution in 1:20 western blocking reagent diluted in TBST at the following concentrations: rabbit polyclonal CIRBP (ProteinTech, 10209-2-AP, 1:1,000) and rabbit polyclonal HADHB (LSBio-LS-C334236, 1:2,500). .. After washing in TBST, the blots were incubated with the appropriate secondary antibody conjugated to horseradish peroxidase and detected with SuperSignal West Pico detection reagent (Thermo Scientific).

Article Title: Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4′-demethylepipodophyllotoxin
Article Snippet: .. After soaking the membrane in TBST (10 mM Tris-HCl, pH 7.5, 150 mM NaCl) for 15 min and blocking with TBST containing 5% nonfat dried milk for 2 h, the complexes of hTop2β-DNA and free hTop2β were detected using specific antibody probe (Proteintech Group, USA) at a 1:10000 dilution. .. The IRDye 800CW Goat anti-rabbit (LI-COR Biosystems, USA) (1:15000) was used as secondary antibody.

Western Blot:

Article Title: Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia
Article Snippet: .. The following day, the membrane was incubated for 1 hr at room temperature with primary antibody solution in 1:20 western blocking reagent diluted in TBST at the following concentrations: rabbit polyclonal CIRBP (ProteinTech, 10209-2-AP, 1:1,000) and rabbit polyclonal HADHB (LSBio-LS-C334236, 1:2,500). .. After washing in TBST, the blots were incubated with the appropriate secondary antibody conjugated to horseradish peroxidase and detected with SuperSignal West Pico detection reagent (Thermo Scientific).

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    Proteintech tbst
    Tbst, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Proteintech
    Average 99 stars, based on 299 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    99/100 stars
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