tapi2  (Millipore)

Journal: iScience

Article Title: Heightened innate immune state induced by viral vector leads to enhanced response to challenge and prolongs malaria vaccine protection

doi: 10.1016/j.isci.2024.111468

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Article Snippet: TAPI2 , Biosynth , Cat #INH-3852-PI.

Techniques: Control, Virus, Recombinant, Giemsa Stain, Cell Stimulation, Transfection, Software

Journal: International Journal of Molecular Sciences

Article Title: HER2 Signaling Implicated in Regulating Alveolar Epithelial Permeability with Cyclic Stretch

doi: 10.3390/ijms20040948

Figure Lengend Snippet: Effect of cyclic stretch (ΔSA 37%, 0.25 Hz, 10 min) and HER pathway inhibition on ( A ) human epidermal growth factor receptor-2 (HER2) activation and ( B ) permeability. ( A ) HER2 activation was measured by Western blot analysis of pHER2 after IP by (separately) anti-HER3 or anti-HER2 antibody. ( B ) Permeability was measured by BODIPY-ouabain fluorescence. Treatment with the NRG1-cleavage inhibitor TAPI2 (50 µM), anti-HER3 antibody (10 µg/mL), and HER2 phosphorylation AG825 (50 µM) mitigated stretch-induced increases in pHER2 and permeability. Values are normalized to unstretched cells. n = 5; p values represent the result of ANOVA and post-hoc Tukey.

Article Snippet: In some experiments, cells were treated for 2 h prior to and during stretch with either the ADAM17 inhibitor TAPI2 (50 μM; Calbiochem, Burlington, MA, USA), competitive anti-HER3 antibody to prevent ligand binding (10 μg antibody/mL DMEM; Abcam, Cambridge, MA, USA), the HER2 tyrosine kinase inhibitor AG825 (50 μM; Calbiochem), or vehicle control (DMSO).

Techniques: Inhibition, Activation Assay, Permeability, Western Blot, Fluorescence, Phospho-proteomics

Journal: International Journal of Molecular Sciences

Article Title: HER2 Signaling Implicated in Regulating Alveolar Epithelial Permeability with Cyclic Stretch

doi: 10.3390/ijms20040948

Figure Lengend Snippet: Effect of cyclic stretch (ΔSA 37%, 0.25 Hz, 10 min) and HER pathway inhibition on tight junction protein expression. After IP using ZO-1, proteins were quantified using Western blot analysis for occluding, and claudins-4, 7, and 18. Treatment with TAPI2 (50 µM) and AG825 (50 µM) reversed stretch-induced decreases in claudin-7. Values are normalized to unstretched cells. n = 5; p values represent the result of ANOVA and post-hoc Tukey.

Article Snippet: In some experiments, cells were treated for 2 h prior to and during stretch with either the ADAM17 inhibitor TAPI2 (50 μM; Calbiochem, Burlington, MA, USA), competitive anti-HER3 antibody to prevent ligand binding (10 μg antibody/mL DMEM; Abcam, Cambridge, MA, USA), the HER2 tyrosine kinase inhibitor AG825 (50 μM; Calbiochem), or vehicle control (DMSO).

Techniques: Inhibition, Expressing, Western Blot