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hy 13782 tenofovir alafenamide taf medchemexpress  (MedChemExpress)


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    MedChemExpress hy 13782 tenofovir alafenamide taf medchemexpress
    Hy 13782 Tenofovir Alafenamide Taf Medchemexpress, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DNA binding of TBP homologs is independent of core domain conservation. ( A ) Gene browser tracks at Gapdh for α-HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control (Ctrl, DMSO) or indole-3-acetic acid (500 μM, IAA)-treated mESCs. Alternative promoters are indicated. ( B ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol II occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all RNA Pol II genes. ( C ) Gene browser tracks at tRNA109-TyrGTA and tRNA110-AlaAGC for HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control or IAA-treated mESCs. ( D ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol III occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all tRNA genes. ( E ) Normalized read counts of IAA-treated HA-yTBP CUT&Tag signal versus IAA-treated HA-mTBP in the promoter (–250 to TSS) region of each Pol II (top) gene and in the gene body of each Pol III (bottom) gene are displayed as scatter plots. ( F ) Co-IP using α-HA or IgG for cells expressing HA-mTBP (left) or HA-yTBP (right) and blotted with α-HA, <t>α-TAF4,</t> and α-BRF1.
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    DNA binding of TBP homologs is independent of core domain conservation. ( A ) Gene browser tracks at Gapdh for α-HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control (Ctrl, DMSO) or indole-3-acetic acid (500 μM, IAA)-treated mESCs. Alternative promoters are indicated. ( B ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol II occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all RNA Pol II genes. ( C ) Gene browser tracks at tRNA109-TyrGTA and tRNA110-AlaAGC for HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control or IAA-treated mESCs. ( D ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol III occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all tRNA genes. ( E ) Normalized read counts of IAA-treated HA-yTBP CUT&Tag signal versus IAA-treated HA-mTBP in the promoter (–250 to TSS) region of each Pol II (top) gene and in the gene body of each Pol III (bottom) gene are displayed as scatter plots. ( F ) Co-IP using α-HA or IgG for cells expressing HA-mTBP (left) or HA-yTBP (right) and blotted with α-HA, <t>α-TAF4,</t> and α-BRF1.
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    DNA binding of TBP homologs is independent of core domain conservation. ( A ) Gene browser tracks at Gapdh for α-HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control (Ctrl, DMSO) or indole-3-acetic acid (500 μM, IAA)-treated mESCs. Alternative promoters are indicated. ( B ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol II occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all RNA Pol II genes. ( C ) Gene browser tracks at tRNA109-TyrGTA and tRNA110-AlaAGC for HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control or IAA-treated mESCs. ( D ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol III occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all tRNA genes. ( E ) Normalized read counts of IAA-treated HA-yTBP CUT&Tag signal versus IAA-treated HA-mTBP in the promoter (–250 to TSS) region of each Pol II (top) gene and in the gene body of each Pol III (bottom) gene are displayed as scatter plots. ( F ) Co-IP using α-HA or IgG for cells expressing HA-mTBP (left) or HA-yTBP (right) and blotted with α-HA, <t>α-TAF4,</t> and α-BRF1.
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    DNA binding of TBP homologs is independent of core domain conservation. ( A ) Gene browser tracks at Gapdh for α-HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control (Ctrl, DMSO) or indole-3-acetic acid (500 μM, IAA)-treated mESCs. Alternative promoters are indicated. ( B ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol II occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all RNA Pol II genes. ( C ) Gene browser tracks at tRNA109-TyrGTA and tRNA110-AlaAGC for HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control or IAA-treated mESCs. ( D ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol III occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all tRNA genes. ( E ) Normalized read counts of IAA-treated HA-yTBP CUT&Tag signal versus IAA-treated HA-mTBP in the promoter (–250 to TSS) region of each Pol II (top) gene and in the gene body of each Pol III (bottom) gene are displayed as scatter plots. ( F ) Co-IP using α-HA or IgG for cells expressing HA-mTBP (left) or HA-yTBP (right) and blotted with α-HA, α-TAF4, and α-BRF1.

    Journal: Nucleic Acids Research

    Article Title: Functional divergence of TBP homologs through distinct DNA-binding dynamics

    doi: 10.1093/nar/gkaf436

    Figure Lengend Snippet: DNA binding of TBP homologs is independent of core domain conservation. ( A ) Gene browser tracks at Gapdh for α-HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control (Ctrl, DMSO) or indole-3-acetic acid (500 μM, IAA)-treated mESCs. Alternative promoters are indicated. ( B ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol II occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all RNA Pol II genes. ( C ) Gene browser tracks at tRNA109-TyrGTA and tRNA110-AlaAGC for HA CUT&Tag of TRF2-HA, TRF3-HA, HA-mTBP, and HA-yTBP in control or IAA-treated mESCs. ( D ) Genome-wide average plots (top) and heatmaps (bottom) arranged by decreasing Pol III occupancy of HA CUT&Tag for TRF2-HA (Ctrl versus IAA), TRF3-HA (Ctrl versus IAA), HA-mTBP (IAA), and HA-yTBP (IAA) in a 2 kb window surrounding the TSS of all tRNA genes. ( E ) Normalized read counts of IAA-treated HA-yTBP CUT&Tag signal versus IAA-treated HA-mTBP in the promoter (–250 to TSS) region of each Pol II (top) gene and in the gene body of each Pol III (bottom) gene are displayed as scatter plots. ( F ) Co-IP using α-HA or IgG for cells expressing HA-mTBP (left) or HA-yTBP (right) and blotted with α-HA, α-TAF4, and α-BRF1.

    Article Snippet: Primary antibodies: anti-HA (α-HA) 1:5000 (EpiCypher 13-2010), α-Tubulin 1:7000 (Abcam ab6046), α-TAF4 1:5000 (Santa Cruz sc-136093), α-BRF1 1:1000 (Santa Cruz sc-390821), α-H3.3 1:5000 (Abnova H3F3B M01), α-Halo 1:3000 (Promega G9211), α-Flag 1:5000 (Sigma M2) α-TBP 1:3000 (mAbcam51841).

    Techniques: Binding Assay, Control, Genome Wide, Co-Immunoprecipitation Assay, Expressing