fluorescent s aureus atcc 49230 strain  (ATCC)

Journal: Synthetic and Systems Biotechnology

Article Title: Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate

doi: 10.1016/j.synbio.2025.05.011

Figure Lengend Snippet: 3HP-production of recombinant S. cerevisiae S2 strain in mineral medium with different concentrations of cerulenin (p < 0.05).

Article Snippet: The total RNA from three recombinant strains was extracted using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) and reverse transcribed into cDNA by ReverTra AceTM qPCR RT Master Mix kit (Toyobo, Tokyo, Japan).

Techniques: Recombinant

Journal: Synthetic and Systems Biotechnology

Article Title: Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate

doi: 10.1016/j.synbio.2025.05.011

Figure Lengend Snippet: Cell growth (A) and 3HP-production (B) of recombinant S. cerevisiae S2 strain in mineral medium with 10 μM cerulenin and different concentrations of acetate (p < 0.05).

Article Snippet: The total RNA from three recombinant strains was extracted using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) and reverse transcribed into cDNA by ReverTra AceTM qPCR RT Master Mix kit (Toyobo, Tokyo, Japan).

Techniques: Recombinant

Journal: Synthetic and Systems Biotechnology

Article Title: Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate

doi: 10.1016/j.synbio.2025.05.011

Figure Lengend Snippet: Growth rate (A) and 3HP-production (B) of recombinant S. cerevisiae S2 strain in rice straw hydrolysate and diluted rice straw hydrolysate with 10 μM cerulenin, whereas RW: rice straw hydrolysate; RW/C: rice straw hydrolysate supplemented with cerulenin; RW/C/M: rice straw hydrolysate supplemented with cerulenin and mineral; diRW/C: diluted rice straw hydrolysate supplemented with cerulenin; diRW/C/M: diluted rice straw hydrolysate supplemented with cerulenin and mineral.

Article Snippet: The total RNA from three recombinant strains was extracted using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) and reverse transcribed into cDNA by ReverTra AceTM qPCR RT Master Mix kit (Toyobo, Tokyo, Japan).

Techniques: Recombinant

Journal: Synthetic and Systems Biotechnology

Article Title: Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate

doi: 10.1016/j.synbio.2025.05.011

Figure Lengend Snippet: Growth rate (A) and 3HP-production (B) of recombinant S. cerevisiae S2 strain in diluted sugar cane juice supplemented with minimum mineral and 10 μM cerulenin, whereas SCJ: sugar cane juice; SCJ + MM: diluted sugar cane juice supplemented with minimum mineral and cerulenin.

Article Snippet: The total RNA from three recombinant strains was extracted using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) and reverse transcribed into cDNA by ReverTra AceTM qPCR RT Master Mix kit (Toyobo, Tokyo, Japan).

Techniques: Recombinant

Journal: Synthetic and Systems Biotechnology

Article Title: Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate

doi: 10.1016/j.synbio.2025.05.011

Figure Lengend Snippet: Growth rate (A) and 3HP-production (B) of recombinant S. cerevisiae S2 strain in diluted molasses supplemented with minimum mineral and 10 μM cerulenin, whereas ML/M/C: diluted molasses supplemented with cerulenin and minimum mineral.

Article Snippet: The total RNA from three recombinant strains was extracted using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) and reverse transcribed into cDNA by ReverTra AceTM qPCR RT Master Mix kit (Toyobo, Tokyo, Japan).

Techniques: Recombinant

Journal: Synthetic and Systems Biotechnology

Article Title: Transcriptomic studies on the product stress response revealed that YCF1 is a beneficial factor for progesterone production in Yarrowia lipolytica

doi: 10.1016/j.synbio.2025.04.008

Figure Lengend Snippet: The response of Y. lipolytica to progesterone stress is reflected mainly in amino acid metabolism, lipid metabolism, and transport. A. Profile of differential gene expression under progesterone stimulation. B. Functional categories of KEGG (left)- and GO (right)-annotated genes that were differentially expressed in response to 15 min of progesterone treatment. C. The highly enriched functional categories of the KEGG-annotated genes found to be induced and repressed after 15 min of progesterone treatment.

Article Snippet: All the Y. lipolytica strains used in this study were derived from ATCC201249 and purchased from the American Type Culture Collection ( ).

Techniques: Gene Expression, Functional Assay

Journal: Synthetic and Systems Biotechnology

Article Title: Transcriptomic studies on the product stress response revealed that YCF1 is a beneficial factor for progesterone production in Yarrowia lipolytica

doi: 10.1016/j.synbio.2025.04.008

Figure Lengend Snippet: The response of Y. lipolytica to progesterone stress is reflected mainly in amino acid metabolism, lipid metabolism, and transport. A. Profile of differential gene expression under progesterone stimulation. B. Functional categories of KEGG (left)- and GO (right)-annotated genes that were differentially expressed in response to 15 min of progesterone treatment. C. The highly enriched functional categories of the KEGG-annotated genes found to be induced and repressed after 15 min of progesterone treatment.

Article Snippet: Genetic transformation of the plasmids into Y. lipolytica strains was conducted with the Frozen-FZ Yeast Transformation IITM Kit (Zymo Research, USA).

Techniques: Gene Expression, Functional Assay